Analysis by SEC/GPC was performed on a ViscotekGPCmax; VE2001 GPC solvent/sample module equipped with triple detector: Refractive Index (RI), Light Scattering (RALS and LALS) and on-line Viscometer. The technique leads to absolute molecular weight as well as data on solution properties. The inert, Mixed Bed ViscoGel I-Series columns: I-MBLMW-3078 and I-MBLMW-3078 (Viscotek) were used to make the measurements. For this purpose, the polymer was dissolved in tetrahydrofuran (THF) at 10 mg/ml at room temperature under soft magnetic shaking for 48 h. After that, 100 μlof each sample was filtered and automatically injected at a flow rate of 0.8 ml/min while the columns and the detector temperature were kept at 30 °C. The determination of the molecular weight distribution was based on the universal calibration technique (UNICAL), which used polystyrene, PS400K and PS65K standards (PolyCAL/Viscotek). Raw data were processed using the OmniSEC GPC/SEC Chromatography System software version 4,5,0,257. Table 1 summarizes the weight average molecular weight (M W ), the polydispersity and the Mark-Houwink exponent, α estimated by this technique.
Linan L.Z., Nascimento Lima N.M., Filho R.M., Sabino M.A., Kozlowski M.T, & Manenti F. (2015). Pilot-scale synthesis and rheological assessment of poly(methyl methacrylate) polymers: perspectives for medical application. Materials science & engineering. C, Materials for biological applications, 51.
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