Human HCC cell lines (BEL-7402, BEL-7404, Huh7, Hep3B, and HepG2) and the normal hepatocyte cell line HL-7702 were purchased from the Shanghai Institute of Cell Biology (Shanghai, China) with a certificate of authenticity for each cell line. The samples were maintained in DMEM/RPMI 1640 (Gibco BRL, Grand Island, NY, United States) with 10% heat-inactivated fetal calf serum, 2 mM l -glutamine, and 100 U/mL penicillin–streptomycin mixture (Gibco BRL, Grand Island, NY, United States) at 37°C in 5% CO2. After the cells were cultured for several months, all cell lines were evaluated for genetic identification prior to the experiments. Based on the appraisal reports provided by CoBioer Biosciences Co., Ltd. (Nanjing, China), no mutations or contaminations were found in our cell lines.
Bel 7404
Manufactured by Shanghai Institute of Biochemistry and Cell Biology
Sourced in China
The BEL-7404 is a laboratory instrument used for cell culture and analysis. It provides a controlled environment for the growth and maintenance of cells, including temperature, humidity, and gas composition regulation. The core function of the BEL-7404 is to support the cultivation and study of various cell types in a laboratory setting.
Automatically generated - may contain errors
Lab products found in correlation
Dmem rpmi1640, by Thermo Fisher Scientific (3 mentions)
Penicillin streptomycin mixture, by Thermo Fisher Scientific (3 mentions)
Bel 7402, by Shanghai Institute of Biochemistry and Cell Biology (3 mentions)
Hl 7702, by Shanghai Institute of Biochemistry and Cell Biology (3 mentions)
Hepg2, by Shanghai Institute of Biochemistry and Cell Biology (3 mentions)
Hep3b, by Shanghai Institute of Biochemistry and Cell Biology (3 mentions)
Huh 7, by Shanghai Institute of Biochemistry and Cell Biology (3 mentions)
L glutamine, by Thermo Fisher Scientific (2 mentions)
3 protocols using bel 7404
1
Authenticated Human Hepatocellular Carcinoma Cell Lines
2
Authenticity Evaluation of Hepatocyte Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The human HCC cell lines (BEL-7402, BEL-7404, Huh7, Hep3B and HepG2) and normal hepatocyte cell HL-7702 were purchased from the Shanghai Institute of Cell Biology with a certificate of authenticity for each cell line. The samples were maintained in DMEM/RPMI1640 (Gibco BRL, Grand Island, NY, USA) supplemented with 10 % heat-inactivated fetal calf serum, 2 mM L-glutamine, and 100 U/mL penicillinstreptomycin mixture (Gibco BRL, Grand Island, NY, USA) at 37 ℃ in 5 % CO2. After undergoing cell culture in our laboratory for several months, all cell lines used were evaluated for genetic identification prior to the experiments. Based on the appraisal reports provided by CoBioer Biosciences Co., Ltd., no mutations and contaminations were found in our cell lines.
3
Characterization of Human Hepatocellular Carcinoma Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The human HCC cell lines (BEL-7402, BEL-7404, Huh7, Hep3B, and HepG2) and normal hepatocyte cells HL-7702 were purchased from the Shanghai Institute of Cell Biology (Shanghai, China) with a certificate of authenticity for each cell line. The samples were maintained in DMEM/RPMI1640 (Gibco BRL, Grand Island, NY, USA) supplemented with 10% heat-inactivated fetal calf serum, 2 mM L-glutamine, and 100 U/mL penicillin-streptomycin mixture (Gibco BRL, Grand Island, NY, USA) at 37°C in 5% CO 2 . After culturing the cells for several months, all cell lines were evaluated for genetic identification prior to the experiments. Based on the appraisal reports provided by CoBioer Biosciences Co., Ltd. (Nanjing, China), no mutations or contaminations were found in our cell lines.
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