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2 protocols using horse radish peroxidase

1

Biochemical Assay Reagents and Protocols

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Thiobarbituric acid (TBA), bovine serum albumin (BSA), Sephadex G-25, and 5,5′ dithiobis 2-nitrobenzoic acid (DTNB) were purchased from Sigma Chemical Co., USA. NN′-bis-acrylamide and NNN′N′-tetramethylethylenediamine (TEMED) were purchased from Merk-Schudart, and reduced glutathione, ascorbic acid, horse radish peroxidase, hydrogen peroxide, 2,4-dinitrophenylhydrazine (DNPH), guanidine hydrochloride, acrylamide, sodium dodecyl sulfate (SDS), ferric chloride, potassium ferricyanide, and ammonium persulfate were obtained from SISCO Research Laboratory, India. Digitonin was procured from Hi-media Ltd., India. All other chemicals used were of analytical grade.
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2

Cellular Antioxidant Activity Assay

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5,5′-Dithiobis-(2-nitrobenzoic acid)-DTNB, ethylene diamine tetra acetic acid (EDTA), horse radish peroxidase (HRP), glutathione reduced, pyrogallol and heparin were obtained from Sisco Research Laboratories (SRL), India. Histopaque-1077, RPMI-1640 medium, low melting agarose and thiobarbituric acid (TBA) were purchased from Sigma Chemicals Co, USA. Trichloro acetic acid (TCA) was obtained from Qualigens Fine Chemicals, India while ethidium bromide (EtBr) from Hi Media, India Ltd. All other chemicals were of highest analytical grade available.
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