Guinea pig anti insulin

Manufactured by Fortrea

Guinea pig anti-insulin is a laboratory reagent used for the detection and quantification of insulin in biological samples. It is a polyclonal antibody raised in guinea pigs against the insulin protein. This product can be used in various immunoassay techniques, such as ELISA, to measure insulin levels in research or clinical settings.

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Lab products found in correlation

Rabbit anti gm130, by Abcam (1 mentions) Rabbit mab anti hsp90, by Cell Signaling Technology (1 mentions) Rabbit anti cyclophilin b, by Thermo Fisher Scientific (1 mentions) Horseradish peroxidase conjugated secondary antibody, by Jackson ImmunoResearch (1 mentions) Enhanced chemi luminescence (ecl), by Merck Group (1 mentions) Mouse anti vinculin, by Merck Group (1 mentions) Protease inhibitors and phosphatase inhibitors cocktails, by Merck Group (1 mentions)

Close spelling variants of this product

Guinea pigs (5 citations)

2 protocols using guinea pig anti insulin

Recombinant Proinsulin Detection Assay

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Chemicals were purchased from Sigma‐Aldrich or ThermoFisher. Recombinant human proinsulin (expressed in E. coli) was purchased from Creative Biomart (Catalog # INS‐315H). Antibodies in this study include rabbit anti‐Myc (Immunology Consultants, RRID:AB_2921297); guinea pig anti‐insulin (Covance, RRID:AB_10013624); rabbit anti‐cyclophilin B (ThermoFisher, RRID:AB_2169138); rabbit polyclonal anti‐calreticulin (Abcam RRID:AB_303402); rabbit mAb anti‐Hsp90 (Cell Signaling, RRID:AB_2233307); rabbit anti‐GM130 (Abcam, RRID:AB_880266); mouse mAb anti‐human proinsulin B‐C junction sequence PKTRREAEDLQVGQ (Abmart, RRID:AB_2921300); mouse mAb anti‐rat proinsulin (CCI‐17, Novus Biologicals RRID:AB_1107982); mouse anti‐rat C‐peptide CII‐29 (Advanced Immunochemical Inc., Catalog ID: 1‐CP‐r); mouse anti rat C‐Peptide I 6H1 (Biorad Catalog # MCA2857) and as a back‐up, a custom‐made rabbit polyclonal antibody against mouse C‐peptide‐2 sequence PQVAQLELGGGPGAGDLQT (Biogot, RRID:AB_2921302).

Arunagiri A., Alam M., Haataja L., Draz H., Alasad B., Samy P., Sadique N., Tong Y., Cai Y., Shakeri H., Fantuzzi F., Ibrahim H., Jang I., Sidarala V., Soleimanpour S.A., Satin L.S., Otonkoski T., Cnop M., Itkin‐Ansari P., Kaufman R.J., Liu M, & Arvan P. (2024). Proinsulin folding and trafficking defects trigger a common pathological disturbance of endoplasmic reticulum homeostasis. Protein Science : A Publication of the Protein Society, 33(4), e4949.

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Islet Protein Extraction and Western Blotting

Cited 1 time

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Hand-picked islets were washed once with PBS and then resuspended in RIPA lysis buffer containing protease inhibitors and phosphatase inhibitors cocktails (Sigma) as described^{32 (link)}. Islets were lysed by repeated pipetting or triturating through a 30G syringe needle. Lysates were resolved on a 4–12% NuPAGE Bis-Tris gel, and then electrotransferred to nitrocellulose membrane for western blotting. Antibodies used were mouse anti-proinsulin (ALPCO), guinea pig anti-insulin (Covance), and mouse anti-vinculin (Millipore). Horseradish peroxidase-conjugated secondary antibodies were from Jackson ImmunoResearch Laboratories, Inc., with protein visualized by ECL (Millipore).

Mao Y., Schoenborn J., Wang Z., Chen X., Matson K., Mohan R., Zhang S., Tang X., Arunagiri A., Arvan P, & Tang X. (2022). Transgenic overexpression of microRNA-30d in pancreatic beta-cells progressively regulates beta-cell function and identity. Scientific Reports, 12, 11969.

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