C57bl 6 wild type male mice

C57BL/6 wild‐type male mice (6–8 weeks old from SPF Biotechnology Co., Ltd, Beijing, China) were given LPS (10 mg kg⁻¹, E. coli O111:B4, Sigma, ‐Aldrich, Sant‐Louis, MO, USA) for 24 h through intratracheal injection to establish the ALI mouse model.^[38] Liposomes (10 mg kg⁻¹) and Nano‐OM (OM: 75 µg kg⁻¹) were administered through the same route 1 h before LPS challenge. All animal studies were conducted following the guidelines of the Institutional Animal Care and Use Committee of Tianjin Medical University (TMUaMEC2020004). Mice were sacrificed 24 h after LPS challenge, and the BALF, lung tissue, and blood serum were collected for further analysis.

C57BL/6 wild-type male mice (6-8 weeks from SPF Biotechnology Co., Ltd, Beijing, China) were used to replicate the ALI mouse model by intratracheal LPS administration. All the surgical procedure was done under the 1% sodium pentobarbital anesthesia (45 mg/kg) through intraperitoneal injection. PW (1.25 nmol/kg) was given through intratracheal injection 2 h before LPS (10 mg/kg) challenge. Mice were sacrificed 24 h after LPS challenge for the analyses of lung inflammation and injury. All mouse studies were performed according to the guidelines of the Institutional Animal Care and Use Committee of Tianjin Medical University.