Frozen PBMCs were once-thawed in a water bath at 37°C prior to use. Cells were washed, re-suspended in a 2% bovine serum albumin solution, and flow cytometry staining was performed with antibodies following manufacturer instructions. The following anti-human antibodies were used: CD45 FITC (HI30, eBioscience), CD163 APC (eBioGHI/61, eBioscience), CD33 FITC (HIM3–4, eBioscience), HLA-DR APC (LN3, eBioscience), CD11b APC-eFluor 780 (ICRF44, eBioscience), PD-L1 PE (MIH1, eBioscience), CD3 PerCP-eFluor 710 (OKT3, eBioscience), CD4 PE (OKT4, eBioscience), CD25 APC (BC96, eBioscience), FoxP3 FITC (PCH101, eBioscience). For assays requiring intracellular stains, cells were permeabilized with a saponin-based Perm/Wash buffer (BD Biosciences). Sample data were collected by flow cytometry on an Attune NxT cytometer with the Attune NxT Software (Thermo Scientific). OneComp eBeads were used for compensation controls (Invitrogen). Fluorescence-minus-one (FMO) controls were used for appropriate gating of subsets. Post-hoc analyses were performed with the FlowJo Software v10.3 (TreeStar).
Foxp3 fitc pch101
Manufactured by Thermo Fisher Scientific
The FoxP3 FITC (PCH101) is a laboratory equipment product. It is used for the detection and analysis of the FoxP3 protein, which is a transcription factor that plays a key role in the development and function of regulatory T cells. This product provides a fluorescein isothiocyanate (FITC) conjugated antibody specific to the FoxP3 protein, allowing for its identification and quantification in various samples.
Automatically generated - may contain errors
Lab products found in correlation
Onecomp ebeads, by Thermo Fisher Scientific (1 mentions)
Hla dr apc ln3, by Thermo Fisher Scientific (1 mentions)
Cd25 apc bc96, by Thermo Fisher Scientific (1 mentions)
Attune nxt software, by Thermo Fisher Scientific (1 mentions)
Attune nxt cytometer, by Thermo Fisher Scientific (1 mentions)
Perm wash buffer, by BD (1 mentions)
Cytofix cytoperm fixation permeabilization kit, by BD (1 mentions)
Foxp3 staining buffer set, by Thermo Fisher Scientific (1 mentions)
Golgistop, by BD (1 mentions)
2 protocols using foxp3 fitc pch101
1
Multiparametric Flow Cytometry of PBMCs
2
Cytokine Production Analysis of T Cell Subsets
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Naïve CD4+ T cells, activated CD25+ Tregs, CD25−LAG3+ T cells, CD25+LAG3+ T cells, and CD4+CD25−LAG3−CD45RA− T cells (Memory CD4+ T cells) were sorted and stimulated for 72 h with anti-CD3ε mAb (10 μg/mL) and anti-human CD28 mAb (5 μg/mL) in the presence of recombinant human IL-2 (100 IU/mL). Twelve hours prior to cytokine production analysis, phorbol 12-myristate 13-acetate (PMA) (25 ng/mL), ionomycin (1 μg/mL) and protein transport inhibitor GolgiStop (BD) were added. After staining with 7-AAD, intracellular staining was performed using Cytofix/Cytoperm Fixation/Permeabilization Kit (BD) following the manufacturer's instructions. For cytokine production analysis, IFN-γ-FITC (4S.B3, eBioscience), IL-4-APC (8D4-8, BioLegend), IL-17A-APC (eBio64DEC17, eBioscience) or IL-10-Alexa Fluor 660 (JES3-9D7, eBioscience) antibodies were used. For staining Foxp3, Foxp3 Staining Buffer Set (eBioscience) and Foxp3-FITC (PCH101, eBioscience) antibody were used.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!