Anthracimycin time-kill kinetics and post-antibiotic effects were
performed in duplicate by broth macrodilution. For the time-kill kinetics
anthracimycin at 0x, 1x, 5x, 10x, or 20x the MIC (MIC = 0.125 mg/L for USA300
MRSA strain TCH1516) was added to CA-MHB in duplicate sterile polystyrene tubes
(Falcon, Bedford MA). The media was then inoculated with ~ 5 x
105 colony-forming units (CFU)/mL in a final volume of 5 mL, and
the tubes were incubated in a 37°C shaking incubator (New Brunswick).
Viable bacteria over time were quantitated by removal of 25 μL aliquots
for serial dilution in phosphate-buffered saline and plating on Todd-Hewitt agar
(Hardy Diagnostics, Santa Maria, CA). Time-kill kinetic studies were performed
in triplicate.
performed in duplicate by broth macrodilution. For the time-kill kinetics
anthracimycin at 0x, 1x, 5x, 10x, or 20x the MIC (MIC = 0.125 mg/L for USA300
MRSA strain TCH1516) was added to CA-MHB in duplicate sterile polystyrene tubes
(Falcon, Bedford MA). The media was then inoculated with ~ 5 x
105 colony-forming units (CFU)/mL in a final volume of 5 mL, and
the tubes were incubated in a 37°C shaking incubator (New Brunswick).
Viable bacteria over time were quantitated by removal of 25 μL aliquots
for serial dilution in phosphate-buffered saline and plating on Todd-Hewitt agar
(Hardy Diagnostics, Santa Maria, CA). Time-kill kinetic studies were performed
in triplicate.