To confirm that the majority of NSLAB belonged to the inoculated adjuncts, Pulsed-Field Gel Electrophoresis (PFGE) was performed as described by Stefanovic et al. (2017a (link)). Isolates from Month 3 samples were analyzed, and the PFGE patterns were compared with the patterns of the three adjuncts (Figure
Violet red bile agar
Violet red bile agar is a culture medium used for the isolation and enumeration of coliforms and Escherichia coli in food, water, and other samples. It contains bile salts and crystal violet, which inhibit the growth of Gram-positive bacteria while allowing the growth of Gram-negative bacteria.
Lab products found in correlation
8 protocols using violet red bile agar
Characterizing NSLAB Dynamics in Ripening Cheese
To confirm that the majority of NSLAB belonged to the inoculated adjuncts, Pulsed-Field Gel Electrophoresis (PFGE) was performed as described by Stefanovic et al. (2017a (link)). Isolates from Month 3 samples were analyzed, and the PFGE patterns were compared with the patterns of the three adjuncts (Figure
Enumeration of Food Microorganisms
Enumeration of Cheese Microbiota
Evaluating Colostrum Bacterial Contamination
Antimicrobial Efficacy of PLA-Nisin Composites
Microbial Strain Cultivation and Polymer Characterization
Bacterial Growth Quantification in Meat
Nutrient Digestibility and Gut Microbiome
[16 ]), crude protein (CP, method 990.03;
[16 ]), ash (method 942.05;
[16 ]), calcium, and phosphorus (method 985.01;
[16 ]). Gross energy was measured by a bomb calorimeter (Model 1261, Parr Instrument Co., Moline, IL), and chromium concentrations of experimental diets and excreta samples were determined with an automated spectrophotometer (Jasco V-650, Jasco Corp., Tokyo, Japan) according to the procedure of Fenton and Fenton
[17 (link)]. The ATTD (%) of nutrients was calculated by the following formula:
Where
Nf = nutrient concentration in feces (%)
Nd = nutrient concentration in diet (%)
Cf = chromium concentration in feces (%)
Cd = chromium concentration in diet (%)
The microbiological assay of excreta was carried out by the procedure suggested by Choi et al.
[18 (link)]. The microbial groups enumerated were total anaerobic bacteria (TAB; plate count agar, Difco Laboratories, Detroit, MI, USA), Bifidobacterium spp. (MRS agar), coliforms (violet red bile agar, Difco Laboratories, Detroit, MI, USA), and Clostridium spp. (Tryptose sulphite cycloserine agar, Oxoid, Hampshire, UK). The anaerobic conditions during the TAB and Clostridium spp. assays were created by using the gas-pak anaerobic system (BBL, No. 260678, Difco, Detroit, MI, USA).
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