5hiaa

Methanol, acetonitrile, 1-octane suphonic acid (HPLC grade, Fisher scientific), EDTA, and sodium dihydrogen orthophosphate (Merck), Metoclopramide (GSK, Pakistan). The neurotransmitter standards (noradrenaline, dopamine, and serotonin) and their metabolites (DOPAC, 5HIAA, and HVA) were purchased from Acros Organics (Belgium). Cisplatin was gifted by the Korea United Pharm (Korea). Bacosides were gifted by the University of Mississippi USA. N-butanol, n-hexane, and acetone were from Haq Chemicals Pakistan.

High-performance liquid chromatography (HPLC) grade acetonitrile (99.9%), methanol (99.9%), 1-octane sulfonic acid sodium salt (>98%) (Fisher Scientific, United Kingdom), sodium dihydrogen orthophosphate (99%) and ethylene diamine tetra acetic acid (≥99%) (EDTA) were purchased from the Merck local distributor in Peshawar, Pakistan. Noradrenaline (≥ 98%), DOPAC (≥98%), dopamine (≥99%), 5HIAA (≥98%), HVA (≥98%), and serotonin (≥99%), were from Acros Organics, Belgium. Cisplatin (≥99.9%) was from Korea United Pharm., Inc. (South Korea). Metoclopramide (MCP; ≥98%) was purchased in solution from GlaxoSmithKline (GSK Pakistan, Ltd.). Commercial grade n-Hexane was from Haq Chemicals Peshawar (Pakistan). The plant was collected at a farm, from Malakand Division (Khyber Pukhtoonkhwa, Pakistan) at its bloom season and was authenticated by Prof. Dr. Muhammad Ibrar, Department of Botany, University of Peshawar, a specimen was preserved in the herbarium for future reference (voucher No. 8717) (Ullah, 2013 ).

High Performance Liquid Chromatography (HPLC) grade acetonitrile, methanol, 1-octane sulphonic acid sodium salt (Fisher scientific U.K.), sodium dihydrogen orthophosphate and ethylene diamine tetra acetic acid (EDTA) were purchased from the Merck local distributor in Peshawar, Pakistan. Noradrenaline, DOPAC, Dopamine, 5HIAA, HVA, serotonin were from Acros organics, Belgium. Cisplatin was from Korea United Pharm. Inc. (Korea) and was dissolved in normal saline at 65 - 70°C. Metoclopramide (MCP) was purchased in solution from GlaxoSmithKline (GSK Pakistan Ltd.). Acetone was from Haq Chemicals Peshawar (Pakistan). The plant rhizome were purchased from a local market at Mardan and was authenticated by Prof. Dr. Muhammad Ibrar, Department of botany, University of Peshawar.