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Br dsdna kit

Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom

The BR dsDNA kit is a laboratory product designed for the quantification of double-stranded DNA (dsDNA) samples. It provides a reliable and accurate method for determining the concentration of dsDNA in a given sample.

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2 protocols using br dsdna kit

1

Reference Genome Long-Read Sequencing

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Genomic DNA was extracted using the Wizard Genomic Purification Kit (Promega, USA). Sample purity and concentration (OD 260/280) were assessed using a Nanodrop 1000 spectrophotometer (ThermoFisher, UK) and Qubit 2.0 fluorometer with the BR dsDNA kit (Invitrogen, UK). For long read sequencing of the reference genome, a sample of cell bank purified stock was cultured and extracted using the Gentra Puregene yeast/bacteria kit according to manufacturer’s instructions (Qiagen, Germany) to produce 30 µg of high molecular weight (>40 kDA) DNA.
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2

High-Quality Yeast Genomic DNA Extraction

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High-quality genomic DNA of yeast for sequencing was extracted using the Qiagen blood and cell culture kit with 100-G or 20-G genomic tips (Qiagen) according to the manufacturer’s instructions. DNA concentration was measured using the BR ds DNA kit (Invitrogen, Carlsbad, CA) and a Qubit 2.0 fluorometer (Thermo Fisher Scientific). The purity was verified with a Nanodrop 2000 UV-Vis spectrophotometer (Thermo Fisher Scientific).
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