Cytokine and chemokine levels in citrated plasma were determined by colourimetric bead assay using the Luminex system and human-specific bead sets (R&D Systems, Minneapolis, MN, USA). Results were interpolated from 5-parameter-fit standard curves generated using the relevant recombinant human proteins (R&D Systems).
Recombinant human proteins
Manufactured by R&D Systems
Recombinant human proteins are laboratory-produced versions of naturally occurring human proteins. They are generated through genetic engineering techniques and can be used for various research and development applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using recombinant human proteins
1
Cytokine and Chemokine Profiling
2
Human Granulosa KGN Cell Culture
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Forskolin (FSK) was purchased from Sigma-Aldrich Co. Ltd. (St. Louis, MO) and recombinant human proteins of BMP-6, -9, -15 and GDF-9 were obtained from R&D Systems Inc. (Minneapolis, MN). Human granulosa KGN cells, originating from a human ovarian granulosa-like tumor cell line [15] [16] [17] , were cultured in DMEM/F12 containing 10% FCS at 37°C in a condition with 5% CO 2 .
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