Ab108198

Manufactured by Abcam

Sourced in United States

Ab108198 is an antibody product offered by Abcam. It is a primary antibody designed for use in various laboratory applications. The core function of this product is to serve as a specific detection reagent.

Automatically generated - may contain errors

Lab products found in correlation

Ab68194, by Abcam (4 mentions) Ab75590, by Abcam (2 mentions) Ab187643, by Abcam (1 mentions) Ab49776, by Abcam (1 mentions) Sc65638, by Santa Cruz Biotechnology (1 mentions) Prestige antibodies hpa051548, by Merck Group (1 mentions) Mabt11, by Merck Group (1 mentions) Sc 1782, by Abcam (1 mentions) Alexa fluor 647, by Merck Group (1 mentions) Hela cervical cancer cell line, by American Type Culture Collection (1 mentions) Ab92336, by Abcam (1 mentions) Af662, by R&D Systems (1 mentions) Rabbit anti phospho histone h3 ser10, by Cell Signaling Technology (1 mentions) Rabbit anti p erk, by Cell Signaling Technology (1 mentions) Rabbit anti phospho mek1 2, by Cell Signaling Technology (1 mentions) A21468, by Thermo Fisher Scientific (1 mentions) Nupage novex 4 12 bis tris gel, by Thermo Fisher Scientific (1 mentions) A5228, by Merck Group (1 mentions) Rabbit anti gapdh, by Cell Signaling Technology (1 mentions) Hematoxylin, by Wuhan Servicebio Technology (1 mentions)

8 protocols using ab108198

Immunostaining of Tight Junction Proteins

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The following primary antibodies and dilutions were used for immunostaining:
mouse α-p120 1:200 (610133; BD Biosciences, Franklin Lakes, NJ); rabbit α-ezrin 1:200 (3145S; Cell Signaling Technology, Danvers, MA); mouse α-γ-actin 1:100 (sc65638; Santa Cruz Biotechnology, Dallas, TX); rabbit α-α-actinin 4 rabbit 1:200 (ab108198; Abcam, Cambridge, MA); mouse α-Rab11a 1:50 (sc166912; Santa Cruz Biotechnology); rabbit α-Crumbs3 1:100 (NBP1-81185; Novus Biologicals, Littleton, CO); mouse α-Pals1 1:200 (sc365411; Santa Cruz Biotechnology); rabbit α-Patj 1:100 (PA5 85444; Thermo Fisher); sheep α-PKCζ/ι 1:500 (AF4465; R&D Systems, Minneapolis, MN); rabbit α-Par3 1:200 (NBP1-88861; Novus Biologicals); rabbit α-Par6 1:200 (ab49776; Abcam); rabbit α-Cdc42 1:100 (ab187643; Abcam); rabbit α–claudin-2 1:30 (Prestige Antibodies HPA051548; Sigma, Saint Louis, MO); rat α–ZO-1 1:200 (MABT11; Millipore, Billerica, MA); rabbit α–ZO-2 1:200 (71140; Invitrogen, Carlsbad, CA); mouse α–occludin 1:200 (33-1500; Invitrogen); and 1:1000 mouse α-cortactin (05-180, clone 4F11; Millipore). For immunostaining of pig intestinal tissues mouse α-γ-actin 1:100 (sc65638; Santa Cruz Biotechnology) and rabbit α–claudin-2 1:30 (Prestige Antibodies HPA051548; Sigma) were used. Secondary antibodies were conjugated to Alexa Fluor 488, Cy3, Alexa Fluor 594, Alexa Fluor 647, or Cy5.

Engevik A.C., Krystofiak E.S., Kaji I., Meyer A.R., Weis V.G., Goldstein A., Coutts A.W., Melkamu T., Saqui-Salces M, & Goldenring J.R. (2021). Recruitment of Polarity Complexes and Tight Junction Proteins to the Site of Apical Bulk Endocytosis. Cellular and Molecular Gastroenterology and Hepatology, 12(1), 59-80.

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Quantification of Actin-associated Proteins

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For western blot analysis, 10% of total IP lysate was used. Primary antibodies recognizing ACTN1 (ab68194), ACTN4 (ab108198), MYH9 (ab75590) from Abcam were used. After incubation with the rabbit secondary antibodies, protein signals were developed using HRP.

García-Padilla C., Domínguez J.N., Lodde V., Munk R., Abdelmohsen K., Gorospe M., Jiménez-Sábado V., Ginel A., Hove-Madsen L., Aránega A.E, & Franco D. (2022). Identification of atrial-enriched lncRNA Walras linked to cardiomyocyte cytoarchitecture and atrial fibrillation. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 36(1), e22051.

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Characterization of Pancreatic and Cervical Cancer Cell Lines

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PANC-1 and BxPC-3 human pancreatic cancer cell lines and HeLa cervical cancer cell line were obtained from the American Type Culture Collection. DanG human pancreatic cancer cells were provided by Daniel Billadeau (Mayo Clinic). PANC-1, DanG, and HeLa cells were cultured in DMEM with 10% fetal bovine serum (FBS) and penicillin/streptomycin, and BxPC-3 cells were cultured in RPMI media with 10% FBS and penicillin/streptomycin. Cell lines were screened for mycoplasma contamination by DAPI staining and PCR.
Antibodies used in this article were as follows: α-actinin 1 (Santa Cruz; sc-1782, and Abcam; ab68194), α-actinin 4 (Abcam; ab108198), Dyn2 (purification described previously; Henley et al., 1998 ), GAPDH (Cell Signaling; D16H11), GST (Santa Cruz; sc-138), GFP (Roche), His epitope tag (Cell Signaling; 27E8), and cortactin (Cao et al., 2003 (link)). Actin was stained using phalloidin-tetramethylrhodamine or phalloidin–Alexa Fluor 647 (Sigma).

Burton K.M., Cao H., Chen J., Qiang L., Krueger E.W., Johnson K.M., Bamlet W.R., Zhang L., McNiven M.A, & Razidlo G.L. (2020). Dynamin 2 interacts with α-actinin 4 to drive tumor cell invasion. Molecular Biology of the Cell, 31(6), 439-451.

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Versatile Western Blot Protocol

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For Western blot analysis, 10 μg of protein were loaded on NuPAGE Novex 4–12% bis-tris Gels (Invitrogen) and processed as described (32 (link)). The following antibodies were used: rabbit anti-FGFR1 (1:500; no. 9740, Cell Signaling Technology), goat anti-OSMRβ (1:500; AF662, R&D Systems), mouse anti-myotrophin (1:500; 611830, BD), rabbit anti-GAPDH (1:5000; no. 2118, Cell Signaling Technology), rabbit anti-pERK (1:500; no. 9101, Cell Signaling Technology), mouse anti-panERK (1:500; 612641, BD), rabbit anti-ACTN1 (1:1000; ab68194, Abcam), rabbit anti-ACTN4 (1:1000; ab108198, Abcam), mouse anti-αSMA (1:1000; A5228, Sigma-Aldrich), rabbit anti–hexokinase I (1:1000; no. 2024, Cell Signaling Technology), mouse anti-MAD2 (1:1000; 610679, BD), mouse anti-Myh (1:1000; LS-B6307, LSBio), mouse anti-myomesin (1:100; clone M5, gift of H. M. Eppenberger), mouse anti-PCNA (1:1000; 555566, Bionity), rabbit anti–phospho–Histone H3 (Ser¹⁰) (1:1000; no. 9701, Cell Signaling Technology), rabbit anti–phospho-MEK1/2 (1:1000; no. 3958, Cell Signaling Technology), mouse anti–Ral A (1:5000; 610222, BD), rabbit anti-RUNX1 (1:1000; ab92336, Abcam), rabbit anti-SDHA (1:1000; no. 5839, Cell Signaling Technology), mouse anti-TIMP1 (1:1000; MAB9801, R&D Systems), and anti-goat Alexa Fluor 594 (1:1000; A-21468, Invitrogen).

Valussi M., Besser J., Wystub-Lis K., Zukunft S., Richter M., Kubin T., Boettger T, & Braun T. (2021). Repression of Osmr and Fgfr1 by miR-1/133a prevents cardiomyocyte dedifferentiation and cell cycle entry in the adult heart. Science Advances, 7(42), eabi6648.

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Immunostaining for Ki-67 and ACTN4

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Immunostaining was performed according to the standard protocols by Servicebio (Wuhan, China). The primary antibodies of Ki-67 (AF0198, Affinity Biologicals) and ACTN4 (ab108198, Abcam, USA) at 4°C for 12h. HRP-labeled goat anti-rabbit IgG H&L were then incubated with sections (ab97080, Abcam, USA) at room temperature, and then visualized with diaminobenzidine (DAB, Servicebio) hematoxylin (Servicebio).

Tong Y., Feng Z., Li Y., Yan C., He W, & Chen X. (2022). LncRNA TP73-AS1 Exacerbates the Non-Small-Cell Lung Cancer (NSCLC) Process via Regulating miR-125a-3p-Mediated ACTN4. Evidence-based Complementary and Alternative Medicine : eCAM, 2022, 4098271.

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Immunostaining of Phospho-Histone H3 in Cardiomyocytes

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CMs were fixed with 4% paraformaldehyde (Solarbio, China) for 15 min at RT. After being washed with PBS, cells were permeabilized with 0.4% Triton X-100 (Solarbio, China) in PBS for 60 min, then blocked with goat serum (Boster, China) for 30 min, and incubated at 4 °C with primary antibodies against phospho-Histone H3 (pH3;1:400; 06-570-AF488; Millipore, USA), α-actinin (1:400; ab108198; Abcam, UK) overnight, and then incubated with Alexa Fluor 488-conjugated or Alexa Fluor 594-conjugated secondary antibody for 1 h. The cells were counterstained with DAPI (1:50; C0065; Solarbio, China) to label the nuclei. The pH3 positive CMs were then evaluated with a confocal laser scanning microscope (FV10i; Olympus, Japan).

Gao X., Li H., Wang X., Ren Z., Tian Y., Zhao J., Qi W., Wang H., Yu Y., Gong R., Chen H., Ji H., Yang F., Ma W, & Liu Y. (2022). Light Emitting Diodes Irradiation Regulates miRNA-877-3p to Promote Cardiomyocyte Proliferation. International Journal of Medical Sciences, 19(8), 1254-1264.

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Quantification of Actin-associated Proteins

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Western Blot Analysis of ACTN4 and GAPDH

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The Western blot was performed by following the previous report [16 (link)]. Primary antibodies of ACTN4 (ab108198, Abcam, USA) and GAPDH (AF7021, Affinity Biologicals) was incubated with the membrane at 4°C overnight. After washing on the second day, HRP-conjugated secondary antibodies were incubated with membranes at 37°C for 2 h. Protein bands were shown and analyzed by an ECL detection kit (Thermo, Waltham, MA, USA) with the chemiluminescence system (Bio-Rad, USA).

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