Irdye 800cw donkey anti rabbit immunoglobulin g igg

Manufactured by LI COR

IRDye 800CW donkey anti-rabbit immunoglobulin G (IgG) is a fluorescently labeled secondary antibody. It is designed to detect and bind to primary rabbit antibodies in various applications.

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Lab products found in correlation

Irdye 800cw donkey anti goat igg, by LI COR (1 mentions) Irdye 800cw goat anti rat igg, by LI COR (1 mentions) Horseradish peroxidase anti mouse sheep, by GE Healthcare (1 mentions) High affinity anti ha rat and anti ha affinity matrix, by Roche (1 mentions) Irdye 680rd donkey anti mouse igg, by LI COR (1 mentions) Odyssey dlx imaging system, by LI COR (1 mentions)

2 protocols using irdye 800cw donkey anti rabbit immunoglobulin g igg

Phospho-specific GPR35 Antibody Characterization

Cited 1 time

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A rabbit phospho-site–specific hGPR35 antiserum pSer³⁰⁰/pSer³⁰³-hGPR35a (catalogue number 7TM0102C), raised against the sequence KAHKpSQDpSLCVTL, and a pSer²⁹⁸/pSer³⁰¹-mGPR35 antiserum (7TM0102B), raised against the sequence TPHKpSQDpSQILSLT have been described previously (5 ). A GPR35 (nonphospho) antibody (cat number 7TM0102N), directed against the distal part of the carboxyl-terminal tail of hGPR35 was developed in collaboration with 7TM Antibodies GmbH. IRDye 800CW donkey anti-rabbit immunoglobulin G (IgG), IRDye 800CW donkey anti-goat IgG, and IRDye 800CW goat anti-rat IgG were from LI-COR Biosciences. Horseradish peroxidase anti-mouse (sheep) was from GE Healthcare. High-affinity anti-HA (rat) and anti-HA affinity matrix were from Roche Diagnostics. Anti-LgBiT mAb (cat number N7100) which is an affinity-purified mouse mAb for detection of LgBiT and LgBiT-fusion proteins by Western blotting was purchased from Promega Corporation. GRK isoform–directed antisera for GRK 2 sc-13143 (C-9) c and GRK 5 sc-518005 (D-9) were from Santa Cruz Biotechnology while GRK 3 CS #80362 (D8G6V) and GRK 6 CS #5878 (D1A4) were from Cell Signaling Technology. Further details are found in Reichel et al. (23 (link)).

Ganguly A., Quon T., Jenkins L., Joseph B., Al-awar R., Chevigne A., Tobin A.B., Uehling D.E., Hoffmann C., Drube J, & Milligan G. (2023). G protein–receptor kinases 5/6 are the key regulators of G protein–coupled receptor 35–arrestin interactions. The Journal of Biological Chemistry, 299(10), 105218.

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Quantification of mFXN and hFXN Levels

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mFXN and hFXN levels in mouse tissue lysate were analyzed by western blot using primary antibodies specific for mFXN (Abcam catalog no. ab219414), hFXN (Invitrogen catalog no. 45-6300), and GAPDH (Abcam catalog no. ab8245). The secondary antibodies used were IRDye 800CW donkey anti-rabbit immunoglobulin G (IgG) (LI-COR, 926-32213) and IRDye 680RD donkey anti-mouse IgG (LI-COR, 926-68072). Images were taken using an Odyssey DLx imaging system (LI-COR). For the oxidative phosphorylation (OXPHOS) complexes expression analysis, the primary antibodies used were anti-GAPDH (Novus Biologicals, 1D4, NB300-221) at 1:1,000 and anti-OXPHOS (Abcam/Mitosciences, MS601-360, MitoProfile Total antibody cocktail) at 1:500. The secondary antibodies were horseradish peroxidase-conjugated mouse or rabbit from Cell Signaling Technologies at 1:2,000. A total of 30 μg were loaded into a 4%–12% NuPAGE Bis-Tris Gel, SDS-PAGE (NP0336). Quantification of the bands was performed using the NIH ImageJ software and normalized to the loading control (GAPDH) (WT, N = 4; FXN-MCK, N = 5–6).

Chang J.C., Ryan M.R., Stark M.C., Liu S., Purushothaman P., Bolan F., Johnson C.A., Champe M., Meng H., Lawlor M.W., Halawani S., Ngaba L.V., Lynch D.R., Davis C., Gonzalo-Gil E., Lutz C., Urbinati F., Medicherla B, & Fonck C. (2024). AAV8 gene therapy reverses cardiac pathology and prevents early mortality in a mouse model of Friedreich’s ataxia. Molecular Therapy. Methods & Clinical Development, 32(1), 101193.

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