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Csb el006328ra

Manufactured by Abcam
Sourced in United States

CSB-EL006328RA is a lab equipment product. It is a device used in scientific research and analysis. The core function of this product is to perform a specific task or measurement required in a laboratory setting. Further details about its intended use or capabilities are not provided, as an unbiased and factual description is required.

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Lab products found in correlation

3 protocols using csb el006328ra

1

Apoptosis Protein Quantification

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The levels of the pro- and antiapoptotic proteins, Bcl-2 and Bax, were determined using ELISA kits purchased from Cusabio (catalogue numbers: CSB-EL006328RA and CSB-E08854r) and BioVision, Inc. (catalogue number: E4513) according to the suppliers' protocols, respectively. The activity of caspase-3 was determined using a calorimetric kit obtained from Sigma-Aldrich (catalogue number CASP3C).
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2

Apoptotic Protein Quantification

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The concentration of apoptotic proteins including cytochrome c, Bax, and Bcl-2 was measured using enzyme-linked immunosorbent assay kits obtained from Cusabio (catalogue number CSB-EL006328RA), BioVision, Inc. (catalogue number E4513), and Cusabio (catalogue number CSB-E08854r) according to the suppliers' protocols, respectively, while caspase-3 activity is measured by using a calorimetric kit (Sigma-Aldrich, CASP3C-1KT) based on the hydrolysis of Ac-DEVD-pNA by caspase-3, resulting in the release of the p-nitroaniline (pNA) moiety. pNA is detected at 405 nm (εmM = 10.5).
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3

Apoptosis Signaling Pathway Analysis

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Cytochrome c, Bax and Bcl-2 were determined using ELISA kits obtained from Cusabio (Wuhan, China; Cat. No. CSB-EL006328RA), BioVision, Inc. (Milpitas, CA, USA; Cat. No. E4513) and Cusabio (Wuhan, China; Cat. No. CSB-E08854r), respectively. Caspase-3 activity was measured using a colorimetric kit (Sigma-Aldrich, St. Louis, MO, USA; Cat. No. CASP3C-1KT).
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