For preparation of the IL-2/JES6-1 complex, 1 μg of recombinant murine IL-2 (ProSpec, Israel) was mixed with JES6-1 (5 μg/mouse, Bio X cell, USA) in sterile PBS with gentle agitation for overnight at 4°C. Freshly prepared IL-2/JES6-1 complex was i.p.-injected into mice once a day for three consecutive days. To in vivo deplete Foxp3+ T cells in Foxp3-DTR mice, mice were i.p.-injected with DT (1 μg/mouse, Sigma-Aldrich) every other day for a week. For in vivo blockade of IL-2 signaling, anti-IL-2 mAb (JES6-1) was produced from hybridoma (clone #JES6-1A12 obtained from ATCC) and anti-IL-2 mAb (200 μg/mouse) was i.p.-injected into mice every other day for a week.
Recombinant murine il 2
Manufactured by ProSpec
Sourced in Israel
Recombinant murine IL-2 is a cytokine protein produced through recombinant DNA technology. It is derived from the mouse interleukin-2 gene. Interleukin-2 is a signaling molecule that plays a key role in the immune response.
Automatically generated - may contain errors
Lab products found in correlation
Anti il 2, by American Type Culture Collection (1 mentions)
Jes6 1, by BioXCell (1 mentions)
Recombinant human interleukin il 2, by Thermo Fisher Scientific (1 mentions)
Dynabeads mouse t activator cd3 cd28, by Thermo Fisher Scientific (1 mentions)
Dynabeads human t activator cd3 cd28, by Thermo Fisher Scientific (1 mentions)
X vivo medium, by Lonza (1 mentions)
Pan t cell isolation kit 2, by Miltenyi Biotec (1 mentions)
2 protocols using recombinant murine il 2
1
Modulating Immune Responses with IL-2 Complexes
2
Isolation and Activation of Human and Mouse T Cells
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To isolate human T cells, peripheral blood mononuclear cells were extracted from whole blood of healthy donors by Ficoll density gradient centrifugation. T cells were enriched with Dynabeads® Human T-Activator CD3/CD28 (Thermo Fisher Scientific, USA) and followed by stimulation for 24–36 h in the X-Vivo medium (Lonza, CH) supplemented with 50 IU/ml recombinant human interleukin (IL)-2 (PeproTech, USA) and then transduced with the lentiviral particles at multiplicity of infection (MOI) = 40. Mouse T cells isolated from spleen and lymph nodes of C57BL/6 mice by the Pan T Cell Isolation Kit II (Miltenyi Biotec) were activated with Dynabeads® Mouse T-Activator CD3/CD28 (Thermo Fisher Scientific) and recombinant murine IL-2 (ProSpec) for 48 h, and then infected with retroviral particles at MOI = 10.
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