Discovery mr750

MRI was performed with a 3 tesla Discovery MR 750 scanner (General Electric, Milwaukee, WI), and PET data were acquired with a Discovery PET/CT 690 (General Electric) at both time points.

All brain MRI scans were acquired on a 3.0T MR scanner (Discovery MR750, GE Medical Systems, USA). Imaging sequences included transverse T1-weighted (T1W), T2-weighted (T2W), T2-fluid attenuated inversion-recovery (FLAIR), and sagittal T1W imaging, with a section of 4–5 mm thick. No enhanced scanning was performed in all cases. The obtained brain MRI images were evaluated by the pediatric neuroradiologists and CP specialists participating in our study. The findings were divided into periventricular white matter injury (PWMI): periventricular leukomalacia (PVL), ventriculomegaly; diffuse brain injuries: subcortical softening foci, myelin dysplasia, cerebral atrophy, basal ganglia/thalamic lesions; focal lesions: focal cerebral ischemia, porencephalia; cerebral dysplasia: dysplasia of the corpus callosum, cerebellar dysplasia; and normal brain MRI imaging.

All magnetic resonance imaging (MRI) data were acquired on a 3.0-Tesla Discovery MR750 scanner (General Electric, Milwaukee, WI) with an eight-channel receive coil. The 3D T1-weighted structural MRI (sMRI) data were obtained using a brain volume (BRAVO) sequence with the following parameters: repetition time (TR) /echo time (TE) /inversion time (TI) = 8.16/3.18/450 ms; field of view (FOV) = 256 mm × 256 mm; matrix = 256 × 256; flip angle (FA) = 12°; slice thickness = 1 mm; and 188 slices. The resting-state pseudo-continuous arterial spin labeling (ASL) data were obtained using a 3D spiral spin-echo sequence and background suppression: TR / TE = 5046/11.09 ms; post labeling delay (PLD) = 2025 ms; FOV = 240 mm × 240 mm; matrix = 128 × 128; flip angle (FA) = 111°; slice thickness = 3 mm; and 50 slices. Moreover, the resting-state functional MRI (rfMRI) data were obtained using a single-shot gradient-recalled-echo echo-planar-imaging (SS-GRE-EPI) sequence: TR/TE = 2000/30 ms; FOV = 220 mm × 220 mm; matrix = 64 × 64; FA = 90°; slice thickness = 3 mm; gap = 1 mm; 36 slices; and 180 volumes.

MRI data were acquired by using a 3.0-Tesla MR system (Discovery MR750, General Electric, Milwaukee, WI, USA). We used tight, comfortable foam padding to reduce subject head movement, and earplugs were used to reduce noise from the scanner. 3D T1-weighted images were acquired by using a brain volume (BRAVO) sequence with the following parameters: repetition time (TR) = 8.16 ms; echo time (TE) = 3.18 ms; inversion time (TI) = 450 ms; flip angle (FA) = 12°; field of view (FOV) = 256 mm × 256 mm; matrix = 256 × 256; slice thickness = 1 mm, no gap; 184 sagittal slices; and acquisition time = 357 s. BOLD images were acquired by using a GRE-SS-EPI sequence with the following parameters: TR/TE = 2,000/35 ms; FOV = 220 mm × 220 mm; matrix = 64 × 64; FA = 90°; slice thickness = 3 mm; gap = 0.5 mm; 36 interleaved transverse slices; 185 volumes; and acquisition time = 370 s. We asked all subjects to close their eyes during the MRI scan, move as little as possible, not think about anything in particular, but not fall asleep. To ensure that only images without visible artifacts were included in the analysis, all MR images were visually inspected.

Structural MRI and DTI data were acquired from each subject on a 3-T MRI scanner (Discovery MR750, GE Medical system, Waukesha, WI, United States). Structural T1-weighted FSPGR BRAVO scans were obtained (120 axial slices, FOV = 512 mm × 512 mm, TR = 7232 ms, TE = 2.78 ms, in-plane voxel dimensions 0.5 mm × 0.5 mm, slice thickness = 1.5 mm). For the DTI protocol, a 12-min DTI (HARDI) scan was collected in 55 diffusion weighted directions (b = 3000 s/mm², TR = 9313 ms, TE = 76.6 ms, FOV = 256 mm × 256 mm, in-plane voxel dimensions = 2 mm × 2 mm, axial slice thickness = 2 mm).