Advance 300

Manufactured by Bruker

Sourced in Spain

The Bruker Advance 300 is a high-performance nuclear magnetic resonance (NMR) spectrometer designed for analytical and research applications. It operates at a magnetic field strength of 7.05 Tesla, providing a proton frequency of 300 MHz. The Advance 300 is capable of performing a variety of NMR experiments to analyze the chemical structure and composition of samples.

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Lab products found in correlation

Sigma 300, by Zeiss (2 mentions) Qnp probe, by Bruker (1 mentions) Lambda 950, by PerkinElmer (1 mentions) Impact hd mass spectrometer, by Bruker (1 mentions) F 4500, by Hitachi (1 mentions) Delsa nano c particle analyzer, by Beckman Coulter (1 mentions) D2 phaser x ray diffractometer, by Bruker (1 mentions) 240c analyzer, by PerkinElmer (1 mentions) Microtof q 2 spectrometer, by Bruker (1 mentions) Vertex 70, by PerkinElmer (1 mentions) B 545, by Büchi (1 mentions) Axis ultra dld spectrometer, by Shimadzu (1 mentions) Fp 6500, by Jasco (1 mentions) S 4700, by Hitachi (1 mentions)

16 protocols using advance 300

Epoxide Quantification by 1H NMR

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¹H NMR spectrum of the synthesized reactive diluents were obtained using Bruker Advance 300 (300 MHz) spectrometer equipped with a QNP probe at room temperature. Acetone-d₆ was used as deuterated solvent. Chemical shifts (δ) are given in ppm referenced to the residual deuterated solvent peak.
The Epoxide Index (EI, meq g⁻¹) was determined according to ¹H NMR titration method (¹H qNMR). The method consists in solubilizing a known mass of the product and of an internal standard (trioxane with a purity of 99.9%). The number of moles of epoxide per gram of product was measured by comparing the standardized integration of the standard with the standardized integration of the oxirane rings.
The epoxy equivalent weight (EEW, g eq⁻¹) was determined thanks to the EI (meq g⁻¹) using the following eqn (4):

Malburet S., Bertrand H., Richard C., Lacabanne C., Dantras E, & Graillot A. (2023). Biobased epoxy reactive diluents prepared from monophenol derivatives: effect on viscosity and glass transition temperature of epoxy resins. RSC Advances, 13(22), 15099-15106.

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Spectroscopic Characterization of Compounds

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All chemicals were purchased from Sigma Aldrich and were used as received without further purification. ¹H and ¹³C NMR spectra were performed on a Bruker Advance 300 spectrometer at 300 MHz, with DMSO-d₆.

, & Al-mahamad L.L. (2019). Synthesis and surface characterization of new triplex polymer of Ag(I) and mixture nucleosides: cytidine and 8-bromoguanosine. Heliyon, 5(5), e01609.

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Comprehensive Characterization of Compounds

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Bruker Advance 300 and 500 MHz instruments were used to measure the nuclear magnetic resonance (NMR) spectra of all compounds at room temperature. The molecular weights of all target compounds were recorded on a Bruker Impact HD mass spectrometer (ESI mode) to obtain the high-resolution mass spectra (HRMS). The elemental analyses were carried out by an Elementar Vario CUBE (CHN-OS Rapid, Germany) instrument. The morphology of compounds was studied on a cold field emission scanning electron microscope (SEM SU8010). The average sizes of all target compounds were determined by a dynamic light scattering (DLS) technique on a DelsaNano C particle analyzer (BECKMAN COULTER). An ultraviolet/visible near-infrared spectrophotometer (Lambda 950, PerkinElmer) between 200 and 800 nm was used to measure absorbance spectra of all compounds. The photoluminescence spectra were recorded with a fluorescence spectrophotometer (HITACHI F-4500). The time-resolved photoluminescence (TRPL) spectra were obtained by a PDL 200 pulsed diode laser.

Nhien P.Q., Cuc T.T., Khang T.M., Wu C.H., Hue B.T., Wu J.I., Mansel B.W., Chen H.L, & Lin H.C. (2020). Highly Efficient Förster Resonance Energy Transfer Modulations of Dual-AIEgens between a Tetraphenylethylene Donor and a Merocyanine Acceptor in Photo-Switchable [2]Rotaxanes and Reversible Photo-Patterning Applications. ACS applied materials & interfaces, 12(42), 47921-47938.

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Characterization of Organic Compounds

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All reagents and solvents were purchased from commercial suppliers (Avra, Sigma, Alfa Aesar) and used without further purification. The reactions were monitored by thin-layer chromatography (TLC) using merck silica gel 60 F254 and visualized by UV detection or using p-anisaldehyde stain or DNP stain or molecular iodine. ¹H NMR spectra were recorded in CDCl₃ at room temperature on a Bruker Advance 300 spectrometer operating at 300 MHz. Chemical shifts (δ) are expressed in ppm using tetramethylsilane (TMS) as internal standard and coupling constants (J) are given in Hz.

Rathore S.S., Isravel M., Vellaisamy S., Chellappan D.R., Cheepurupalli L., Raman T, & Ramakrishnan J. (2017). Exploration of Antifungal and Immunomodulatory Potentials of a Furanone Derivative to Rescue Disseminated Cryptococosis in Mice. Scientific Reports, 7, 15400.

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Multimodal Characterization of Materials

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The crystallographic data of samples were obtained by a Bruker D2 Phaser X‐Ray Diffractometer (XRD). The Fourier transform infrared spectroscopy (FTIR) mapping was performed with the Nicolet iS50 FT‐IR Instrument. Raman spectroscopy (DXR3x) was collected with a 532 nm laser. ¹H spectra were obtained by a Bruker Advance 300. The microscopic morphology was observed by scanning electron microscope (ZEISS Sigma 300). Dendrite growth was observed in situ with Nreeohy metallographic microscope. The ionic conductivity was tested by Shanghai Thundermagnetic DDBJ‐350 portable conductivity tester.

Yang J., Han Z., Wang Z., Song L., Zhang B., Chen H., Li X., Lau W, & Zhou D. (2023). Enabling Stable Zn Anodes by Molecularly Engineering the Inner Helmholtz Plane with Amphiphilic Dibenzenesulfonimide Additive. Advanced Science, 10(22), 2301785.

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NMR Characterization of Imidazole-2-ylidene Silver(I) Complexes

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Reagents and solvents. All chemicals were commercially available (TCI chemicals) and were used as received unless stated otherwise. The solvents were dried and distilled before use. NMR Analysis: the NMR spectra were recorded on Bruker Advance 300, 400, and 600 MHz spectrometers at 25 °C. The ¹H and ¹³C NMR chemical shifts are referenced to SiMe₄ (δ=0 ppm) using the residual proton impurities of the deuterated solvents as internal standards. ¹H NMR spectra are referenced using the residual solvent peak δ 7.27 for CDCl₃, δ 5.32 for CD₂Cl_2, and δ 2.50 for DMSO‐d₆. ¹³C NMR spectra are referenced using the residual solvent peak at δ 77.23 for CDCl₃, δ 54.00 for CD₂Cl_2, and δ 39.51 for DMSO‐d₆. Chemical shifts (δ) are expressed as parts per million. Multiplicities are abbreviated as follows: singlet (s), doublet (d), triplet (t), quartet (q), multiplet (m), and broad (br). Elemental analysis was recorded on a with a PERKIN‐Elmer 240‐C analyzer.
The complexes [N‐methyl,N’‐[(2‐sodiumalcholate‐2‐phenyl)ethyl])‐imidazole‐2‐ylidine]silver(I)] iodide (1) and [N‐methyl,N’‐(2‐metoxy‐2‐phenyl) ethyl‐imidazole‐2‐ylidene silver(I)] iodide (3) were obtained following the procedure reported in rif..^[20]

Mariconda A., Iacopetta D., Sirignano M., Ceramella J., Costabile C., Pellegrino M., Rosano C., Catalano A., Saturnino C., El‐Kashef H., Aquaro S., Sinicropi M.S, & Longo P. (2022). N‐Heterocyclic Carbene (NHC) Silver Complexes as Versatile Chemotherapeutic Agents Targeting Human Topoisomerases and Actin. Chemmedchem, 17(18), e202200345.

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NMR Characterization of Compounds

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The chemicals were acquired from Sigma-Aldrich Co. in adequate purity to use in the synthetic procedures. The obtained compounds were characterized through ¹H and ¹³C NMR spectroscopy in a Bruker Advance 300 spectrometer, operating at 300 and 75 MHz frequency, respectively, using TMS as internal standard and the chemical shifts (δ) are presented in ppm.

Varela M.T., Romanelli M., Amaral M., Tempone A.G, & Fernandes J.P. (2023). Piperazine amides with desirable solubility, physicochemical and drug-like properties: Synthesis and evaluation of the anti-Trypanosoma cruzi activity. Saudi Pharmaceutical Journal : SPJ, 31(7), 1265-1273.

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Solid-State NMR Characterization of Cement

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Example 7

The cement samples obtained after 7 days incubation were studied using solid-state magic angle spinning (MAS) NMR spectrometry. The experiments were carried out on a Bruker Advance 300 spectrometer, operating at 7.0 T (¹H and ³¹P Larmor frequencies of 300 and 121.5 MHz), using 4 mm double-resonance and triple-resonance MAS probes.

The ³¹P-{¹H} cross-polarisation (CP) MAS experiments were performed using a ramped cross polarization with a contact time of 1 ms. ¹H decoupling was achieved using the SPINAL64 sequence with a ¹H nutation frequency of 70 kHz. The recycle delay was set to 2 s. Longitudinal relaxation times T₁for ³¹P sites in the modified α-TCP samples were measured and found to vary between 10 and 300 s (v₀(³¹P)=121.5 MHz). The ³¹P single pulse spectra were thus obtained by recording a single scan after a delay of 600 s.

US09913928B2. Injectable calcium-phosphate cement releasing a bone resorption inhibitor (2018-03-13). GRAFTYS [FR], UNIVERSITE DE NANTES [FR], CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (C.N.R.S.) [FR]. Inventors: Jean-Michel Bouler [FR], Bruno Bujoli [FR], Pascal Janvier [FR], Ibrahim Khairoun [FR], Jean-Noël Argenson [FR].

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NMR Characterization of Organic Molecules

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Samples were dissolved in deuterated dichloromethane in 5 mm fully dry NMR tubes. For NMR spectroscopy, samples were degassed in ultrasonicator for 10 min before analysis. Tetramethylsilane was utilized as an internal standard^{30 (link)}. For the characterization of organic molecules, measurements with a relaxation delay of 6 s were carried out in a Bruker Advance 300 spectrometer operating at a magnetic field strength of 400 MHz^{28 (link)}.

Singh A., Singh S., Kansal S.K., Garg M, & Krishania M. (2023). Production and characterization of anthocyanin-rich beer from black wheat by an efficient isolate Saccharomyces cerevisiae CMS12. Scientific Reports, 13, 5863.

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Synthesis and Characterization of RGD Peptide-Functionalized Polymers

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All chemicals and solvents were purchased from Sigma-Aldrich, Fisher Scientific, or Oakwood Chemical and used without further purification unless noted otherwise. CYCLO(ARG-GLY-ASP-D-PHE-CYS) (RGD-SH) was purchased from AstraTech. RGD-PEG-SH was purchased from Biochempeg Scientific. Isopropyl glycidyl ether (iPGE, 98%) and ethyl glycidyl ether (EGE, 98%) were dried over CaH2 for 24 h, distilled into a flask containing butyl magnesium chloride (2 M in tetrahydrofuran, THF), re-distilled, and stored under N₂ atmosphere. Poly(ethylene oxide) (PEO, Mn 8000 g mol-1) was dried under vacuum overnight prior to use. Dry THF was obtained using neutral alumina using a Pure Process Technology solvent purification system. A potassium naphthalenide solution (1M) was prepared by dissolving naphthalene (3.2 g) in THF (25 mL), adding potassium (0.975 g), and storing under N₂ atmosphere. All NMR spectra were obtained on a Bruker Advance 300 or 500 MHz spectrometer. All absorbance spectra were collected on either a Varian Cary 5000 UV-Vis-NIR or Agilent 8453 UV-Vis spectrophotometer.

Vordermeier H.M., Cockle P.C., Whelan A., Rhodes S., Palmer N., Bakker D, & Hewinson R.G. (1999). Development of Diagnostic Reagents To Differentiate between Mycobacterium bovis BCG Vaccination and M. bovis Infection in Cattle. Clinical and Diagnostic Laboratory Immunology, 6(5), 675-682.

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