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Falcon labware

Manufactured by BD
Sourced in United States

Falcon Labware is a line of laboratory equipment and consumables manufactured by BD. The products are designed for various applications in scientific research and clinical settings. They provide fundamental functions such as sample collection, processing, and storage. Falcon Labware offers a range of products, including culture dishes, centrifuge tubes, pipette tips, and other essential laboratory tools.

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15 protocols using falcon labware

1

Optimized Reagent Purchasing Protocol

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Unless specified otherwise, chemicals and laboratory consumables were purchased from Sigma-Aldrich Co. LLC (St. Louis, MI, USA) and Falcon Labware (BD Biosciences, Franklin Lakes, NJ, USA), respectively.
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2

Immortalized Corneal Epithelial Cell Line

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The parental human telomerase-immortalized corneal epithelial (hTCEpi) cell line has been characterized previously [14 (link)]. We obtained this cell line from Dr. Jester’s laboratory at the University of California Irvine. hTCEpi cells were routinely maintained in serum-free keratinocyte growth media (KSFM) (with 0.15 mM Ca2+) supplemented with bovine pituitary extract (BPE), insulin, hydrocortisone, penicillin, streptomycin, amphotericin B and human epidermal growth factor (hEGF) (KGM™-CD from Lonza Clonetics, Walkersville, MD). Unless specified elsewhere, cells were passaged every 4 days using 0.25% trypsin-EDTA and 0.25 mg/ml soybean trypsin inhibitor (Invitrogen, Carlsbad, CA) and cultured in T75 tissue culture flasks (Falcon Labware; BD Biosciences, Bedford, MA) incubated at 37°C in 5% CO2.
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3

Ferulic Acid Antioxidant Activity in Mice

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Ferulic acid was purchased from Sigma-Aldrich Co. LLC (CAS:537-98-4; St. Louis, MI, USA), and 2’,7’-dichlorodihydrofluorescein-diacetate (DCF-DA) was from Abcam (Cambridge, UK). Fetal bovine serum (FBS) was purchased from HyClone Laboratories (Logan, UT, USA). Unless specified otherwise, other chemicals and laboratory consumables were purchased from Sigma-Aldrich Co. LLC and Falcon Labware (BD Biosciences, Franklin Lakes, NJ, USA), respectively. C57BL/6 mice (6 weeks old) were purchased from Damul Science (Daejeon, Republic of Korea) and equilibrated for 7 days before use. During the experimental period, all mice were housed at 22 ± 1 °C and 55 ± 5% humidity, along with 12 h light/dark autocycle, allowing ad libitum feeding in the Animal Center of School of Dentistry, Jeonbuk National University.
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4

Biochemical Analysis of Apoptosis Pathways

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Unless otherwise specified, all chemicals and laboratory plastic wares were purchased from Sigma-Aldrich (St. Louis, MO, USA) and Falcon Labware (Becton, Dickinson and Company, Franklin Lakes, NJ, USA), respectively. RPMI 1640 medium, fetal bovine serum (FBS), and antibiotics were purchased from Gibco/Invitrogen (Thermo Fisher Scientific Inc., Waltham, MA, USA). Mouse or rabbit monoclonal antibodies specific for caspase-3 (Cat. No.: #9661), caspase-7 (Cat. No.: #9491), caspase-9 (Cat. No.: #9508), cytochrome c (Cat. No.: #11940), GRP 78 (Cat. No.: #3183), GRP 94 (Cat. No.: #2104), CHOP (Cat. No.: #2895), XBP-1 (Cat. No.: #40435), phosphorylated (p)-ERK1/2 (Cat. No.: #4377), p-p38 (Cat. No.: #9216), p-Akt (Cat. No.: #9271), ERK1/2 (Cat. No.: #9102), p38 (Cat. No.: #8690), Akt (Cat. No.: #9272), and β-actin (Cat. No.: #8457), and secondary antibodies [horseradish peroxidase (HRP)-conjugated anti-mouse IgG (Cat. No.: #7076) or anti-rabbit IgG (Cat. No.: #7074)] were purchased from Cell Signaling Technology (Danvers, MA, USA). The antibody specific for caspase-12 (Cat. No.: sc-21747) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies specific for p-JNK (Cat. No.: AP0473) and JNK-1 (Cat. No.: A5051) were purchased from ABclonal (Woburn, MA, USA).
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5

Culturing Cells in DMEM and FBS

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Unless specified otherwise, all chemicals and laboratory wares were purchased from Sigma Chemical Co., (St. Louis, MO, USA) and Falcon Labware (Becton-Dickinson, Franklin Lakes, NJ, USA), respectively. Dulbecco’s modified Eagle’s medium (DMEM) and fetal bovine serum (FBS) were purchased from Gibco Co., (Gibco BRL, New York, NY, USA).
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6

Osteogenic Pathway Regulation Analysis

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Fetal bovine serum (FBS) was purchased from Gibco-BRL (Gaithersburg, MD, USA). Anti-COLIA, OPN, OCN, OSX, RUNX2, LXRα, LXRβ, Smo, Gli1, β-actin, goat anti-mouse, and goat anti-rabbit antibodies were supplied by Santa Cruz Biotechnology (Santa Cruz, CA, USA). Unless otherwise specified, chemicals and laboratory wares were from the Sigma Chemical Company (St Louis, MO, USA) and Falcon Labware (Becton-Dickinson, Franklin Lakes, NJ, USA), respectively.
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7

Ag NPs and L-Arg Interaction Analysis

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Silver nanoparticles (Ag NPs) and L-Arg, were obtained from Sigma-Aldrich (Sigma-Aldrich, St. Louis, MO, USA). Antibodies were purchased from ENZO Life Science (Farmingdale, NY, USA). Chemiluminescence for band detection was bought from Thermo Scientific (Rockford, IL, USA). All laboratory glassware was acquired from Falcon Labware (Becton, Dickinson and Company, Franklin Lakes, NJ, USA).
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8

Cell Culture with Bovine Serum

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Fetal bovine serum (FBS) was purchased from Gibco-BRL (Gaithersburg, MD, USA). Collagen type I (COLIA), osteopontin (OPN), β-actin, goat anti-mouse, and goat anti-rabbit antibodies were supplied by Santa Cruz Biotechnology (Santa Cruz, CA, USA). Unless otherwise specified, chemicals and laboratory wares were from Sigma Chemical Company (St. Louis, MO, USA) and Falcon Labware (Becton-Dickinson, Franklin Lakes, NJ, USA), respectively.
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9

Molecular Mechanisms of Apoptosis Signaling

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Unless otherwise specified, all chemicals (including PQ) and laboratory plastic ware were purchased from Sigma-Aldrich (St. Louis, MO, USA) and Falcon Labware (Becton, Dickinson and Company, Franklin Lakes, NJ, USA), respectively. Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), and antibiotics (penicillin–streptomycin (Cat. No.: 15140122), and gentamicin (Cat. No.: 15710064)) were purchased from Gibco/Invitrogen (Thermo Fisher Scientific, Waltham, MA, USA). Mouse- or rabbit- monoclonal antibodies specific to cleaved caspase-3 (Cat. No.: #9661), cleaved caspase-7 (Cat. No.: #9491), PARP (Cat. No.: #9542), cytochrome c (Cat. No.: #11940), phosphorylated (p)-JNK (Cat. No.: #9255), p-ERK1/2 (Cat. No.: #4377), p-p38 (Cat. No.: #9216), JNK-1 (Cat. No.: #3708), ERK1/2 (Cat. No.: #9102), p38 (Cat. No.: #8690), and β-actin (Cat. No.: #8457), and secondary antibodies [horseradish peroxidase (HRP)-conjugated anti-mouse IgG (Cat. No.: #7076) or anti-rabbit IgG (Cat. No.: #7074) were purchased from Cell Signaling Technology (Danvers, MA, USA).
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10

Cytokine Stimulation of Cell Cultures

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Unless otherwise stated, chemicals and laboratory wares were purchased from Sigma Chemical Co. (St. Louis, MO, USA) and Falcon Labware (Becton-Dickinson, Franklin Lakes, NJ, USA). Dulbecco’s modified Eagle medium (DMEM), fetal bovine serum, and recombinant human IL-6 (PHC0064) were purchased from Gibco (Grand Island, NY, USA). Recombinant mouse TNF-α (Cat. No. Z02918-20) and human IL-1β (Cat. No. Z02922-10) were purchased from GeneScript (Piscataway, NJ, USA).
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