Rabbit anti rps6

Manufactured by Abcam

Rabbit anti-RPS6 is a primary antibody that specifically recognizes the ribosomal protein S6 (RPS6) in rabbit samples. RPS6 is a component of the 40S subunit of the eukaryotic ribosome and plays a role in the regulation of cell growth and proliferation.

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Lab products found in correlation

4 protocols using rabbit anti rps6

Western Blot Analysis of Protein Markers

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Proteins were separated by SDS-PAGE and immunoblotted with the following antibodies: rabbit anti-JUND (Santa Cruz, sc-74), mouse anti-ATF4 (Santa Cruz, sc-390063), rabbit anti-cleaved caspase-3 (Cell Signaling, 9664S), mouse anti-Tubulin (Sigma, T9026), mouse anti-Ran (B.D. 610340), goat anti-GFP (Abcam, 6673), mouse anti-RPL10A (Novus, 3G2), rabbit anti-RPL7 (Novus, NB100-2269), and rabbit anti-RPS6 (Abcam, ab40820).

Good A.L., Cannon C.E., Haemmerle M.W., Yang J., Stanescu D.E., Doliba N.M., Birnbaum M.J, & Stoffers D.A. (2019). JUND regulates pancreatic β cell survival during metabolic stress. Molecular Metabolism, 25, 95-106.

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Glioma Tissue Analysis by Western Blot and IHC

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For western blot analysis, resected glioma tissues were harvested from 12 patients (seven males, five females, low‐grade: six cases, high‐grade: six cases) at Kumamoto University Hospital, Japan, after having obtained informed consent from the patients and in accordance with the guidelines of the Research Ethics Committee (Kumamoto University Hospital No. 1470). All samples were rapidly frozen after surgery and stored at − 80°C. Demographic data of the patients are provided in Table 1.
For immunohistochemical analysis, tumor tissues were fixed in formalin overnight, embedded in paraffin, and then cut into 3‐μm‐thick sections. The following antibodies were used to detect antigens: rabbit anti‐RPS6 (1:100; Abcam), rabbit anti‐GFAP (1:4000; DAKO), rabbit anti‐SOX2 (1:100; CST), rabbit anti‐Nestin (1:200; Merck), rabbit anti‐Olig2 (1:100; IBL), and mouse anti‐MAP2 (1:5000; Sigma) antibodies. Reactions were visualized using a diaminobenzidine substrate system (Nichirei).

Shirakawa Y., Hide T., Yamaoka M., Ito Y., Ito N., Ohta K., Shinojima N., Mukasa A., Saito H, & Jono H. (2020). Ribosomal protein S6 promotes stem‐like characters in glioma cells. Cancer Science, 111(6), 2041-2051.

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Comprehensive Antibody Panel for Cellular Protein Analysis

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The antibodies used in this study included: mouse anti-GAPDH (Santa Cruz Biotechnology, sc-365,062), goat anti-TFE3 (Santa Cruz Biotechnology, sc-5958), rabbit anti-TFE3 (Abcam, ab93808), mouse anti-4EBP3 (Santa Cruz Biotechnology, sc-134,232), rabbit anti-pS6 (Cell Signaling Technology, 2215), rabbit anti-RPS6 (Abcam, ab40820), rabbit anti-mTOR (Cell Signaling Technology, 2972), rabbit anti-14-3-3 β/α (Cell Signaling Technology, 9636), rabbit anti-14-3-3 γ (Cell Signaling Technology, 5522), rabbit anti-14-3-3 ζ/δ (Cell Signaling Technology, 7413), rabbit anti-14-3-3 ε (Cell Signaling Technology, 9635), rabbit anti-14-3-3 τ (Cell Signaling Technology, 9638), rabbit anti-14-3-3 η (Cell Signaling Technology, 5521), rabbit anti-LAMP2 (Cell Signaling Technology, 49,067), rabbit anti-β-Tubulin (Cell Signaling Technology, 2146), HRP-conjugated goat anti-rabbit (Cell Signaling Technology, 7074), goat anti-mouse secondary antibody (Boster, BA1050), rabbit anti-goat secondary antibody (Boster, BA1060), Alexa Fluor 488-conjugated goat anti-rabbit (Abcam, ab150077), and Alexa Fluor 594-conjugated donkey anti-goat secondary antibodies (Invitrogen, A-11058).

Yin X., Wang B., Gan W., Zhuang W., Xiang Z., Han X, & Li D. (2019). TFE3 fusions escape from controlling of mTOR signaling pathway and accumulate in the nucleus promoting genes expression in Xp11.2 translocation renal cell carcinomas. Journal of Experimental & Clinical Cancer Research : CR, 38, 119.

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Fo-4 Western Blotting Optimization

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For western blotting of Fo-4, hyphae were treated with different concentrations of SA and total protein extracts were prepared with RIPA lysis buffer. Primary antibodies were used: rabbit anti-phospho-AMPKα T172 (#2535; Cell signaling technology), mouse anti-AMPKα (MCA2672GA; BIO-RAD), rabbit anti-phospho-RAPTOR S792 (#2083; Cell signaling technology), rabbit anti-RAPTOR (#2280; Cell signaling technology), rabbit anti-phospho-S6 ribosomal protein S235/236 (#2211; Cell signaling technology), rabbit anti-RPS6 (ab40820; Abcam), and mouse anti-yeast β-actin (AT0014; CMCTAG). The integrated optical density values of the indicated bands were quantified using the software Image-Pro Plus.

Li L., Zhu T., Song Y., Feng L., Kear P.J., Riseh R.S., Sitohy M., Datla R, & Ren M. (2021). Salicylic acid fights against Fusarium wilt by inhibiting target of rapamycin signaling pathway in Fusarium oxysporum. Journal of Advanced Research, 39, 1-13.

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