IHC assays were performed as previously described [13 (link)]. For primary antibody incubation, anti-PTBP3 (Santa Cruz Biotechnology) antibody was applied at 1:100 dilution, anti-HIF-1α (MA1–16511, Thermo Fisher) antibody at 1:100 dilution, anti-CD31 (11265–1-AP, Proteintech, China) antibody at 1:50 dilution, anti-VEGF (Abcam) antibody at 1:100 dilution.
Anti cd31 11265 1 ap
Manufactured by Proteintech
Sourced in China
Anti-CD31 (11265–1-AP) is a primary antibody that recognizes the CD31 protein. CD31, also known as PECAM-1, is a cell adhesion molecule expressed on the surface of endothelial cells, platelets, and certain types of leukocytes. The core function of this antibody is to specifically detect and bind to the CD31 protein for research applications.
Automatically generated - may contain errors
Lab products found in correlation
Anti vegf, by Abcam (1 mentions)
Anti beta actin 20536 1 ap, by Proteintech (1 mentions)
Anti α sma 14395 1 ap, by Proteintech (1 mentions)
Anti e cadherin 20874 1 ap, by Proteintech (1 mentions)
Anti vimentin 10366 1 ap, by Proteintech (1 mentions)
Horseradish peroxidase, by Proteintech (1 mentions)
Anti ha antibody, by Roche (1 mentions)
Anti flag f3165, by Merck Group (1 mentions)
Ab75508, by Abcam (1 mentions)
Ab205718, by Abcam (1 mentions)
Proliferating cell nuclear antigen (pcna), by Proteintech (1 mentions)
4 protocols using anti cd31 11265 1 ap
1
Comprehensive IHC Assay Protocol
2
Comprehensive Western Blotting Protocol
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Western blotting was conducted in accordance with previously described standard protocols [16 (link)]. Primary antibodies, including anti-Beta Actin (20536-1-AP, Proteintech, China), anti-α-SMA (14395-1-AP, Proteintech, China), anti-CD31 (11265-1-AP, Proteintech, China), anti-E-cadherin (20874-1-AP, Proteintech, China), anti-Vimentin (10366-1-AP, Proteintech, China), anti-ZEB1 (21544-1-AP, Proteintech, China), anti-HIF1α (20960-1-AP, Proteintech, China), anti-HIF1α-OH402 (ab72775, Abcam, USA), anti-HIF1α-OH564 (#3434, CST, USA), anti-PHD1 (12984-1-AP, Proteintech, China), anti-PHD2 (19886-1AP, Proteintech, China), and anti-PHD3 (18325-1-AP, Proteintech, China) were used. Secondary antibodies, consisting of goat anti-mouse and anti-rabbit antibodies conjugated with horseradish peroxidase (Proteintech, China), were utilized, and the blots were detected utilizing enhanced chemiluminescence (ECL) (P10300, NCM, China). Quantitative analysis of western blotting was performed using ImageJ.
3
Antibody Utilization in Biological Research
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Following antibodies were used in the experiments: anti-Flag (F3165) from Sigma–Aldrich; anti-GFP (11814460001), anti-Myc (11667149001) and anti-HA antibody (11583816001) from Roche Applied Science; anti-ZBRK1(ab77085) from Abcam; anti-VHL (#2738) from Cell Signaling Technology; anti-VHL (D-7) and anti-p300 (N-15) from Santa Cruz Biotechnology; anti-GAPDH (CW0100) purchased from Beijing CWBio; anti-CD31 (11265-1-AP) purchased from Proteintech. Goat anti-mouse IgG horseradish peroxidase (HRP)-linked whole antibody (SA1-74039) and goat anti-rabbit IgG horseradish peroxidase (HRP)-linked whole antibody (SA1-9510) purchased from Pierce Company (Rockford, IL, USA). FITC-labeled or Cy3-labeled goat anti-rabbit or mouse IgG purchased from CWBIO.
4
Immunohistochemical Analysis of Tumor Samples
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Clinical and mice tumor tissue samples were sectioned into 5 μm-thick slices, deparaffinized in xylene, and rehydrated in a series of graded alcohols. The antigen retrieval was performed by immersing the slides in sodium citrate. The endogenous peroxidase was blocked by a 10-min incubation with 3% H2O2. Next, the slices were incubated with primary antibody anti-mPRα (ab75508, Abcam, Cambridge, UK), anti-CD31 (11265–1-AP, Proteintech, Wuhan, China), ki-67 (27309–1-AP, Proteintech), or PCNA (10205–2-AP, Proteintech) overnight at 4 °C, washed thrice by PBS, and incubated with horse-radish peroxidase (HRP)-conjugated secondary antibody (ab205718, Abcam) for 30 min. Finally, the immunostaining was performed using a DAB (diaminobenzidine) Substrate Kit (ab64238; Abcam).
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