We obtained calcium chloride (CaCl2), sodium chloride (NaCl), and alginic acid sodium salt powder from Sigma-Aldrich (Tokyo, Japan). Sucrose was purchased from China National Pharmaceutical Group Co., Ltd. (Sinopharm, Beijing, China). Fibrinogen and thrombin were purchased from Beijing Solarbio Science and Technology Co., Ltd. (Beijing, China). All chemicals were used without further purification. Three types of non-steroidal anti-inflammatory drugs (aspirin, indomethacin, and nimesulide) were purchased from Sigma-Aldrich (Tokyo, Japan).
Sucrose
Manufactured by Sinopharm
Sourced in China, United States
Sucrose is a disaccharide composed of glucose and fructose. It is a common sweetener and a key ingredient in many laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Sodium chloride (nacl), by Sinopharm (13 mentions)
Potassium chloride (kcl), by Sinopharm (9 mentions)
Methanol, by Sinopharm (8 mentions)
Sodium hydroxide (naoh), by Sinopharm (8 mentions)
Lactose, by Sinopharm (8 mentions)
Fructose, by Sinopharm (7 mentions)
Ethanol, by Sinopharm (6 mentions)
Citric acid, by Sinopharm (5 mentions)
Maltose, by Sinopharm (5 mentions)
Acetone, by Sinopharm (4 mentions)
Hydrochloric acid (hcl), by Sinopharm (4 mentions)
Galactose, by Sinopharm (4 mentions)
Xylose, by Sinopharm (4 mentions)
Cacl2, by Sinopharm (4 mentions)
Bovine serum albumin (bsa), by Merck Group (3 mentions)
Sulfuric acid, by Sinopharm (3 mentions)
Ammonium dihydrogen phosphate, by Sinopharm (3 mentions)
L cysteine, by Sinopharm (3 mentions)
Mannose, by Sinopharm (3 mentions)
Sodium carbonate, by Sinopharm (3 mentions)
72 protocols using sucrose
1
Biomaterial Synthesis with Bioactive Compounds
2
Quantitative Detection of CRP and PCT
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Goat anti-mouse IgG, mouse monoclonal CRP antibody (CRP135 mAb), mouse monoclonal PCT antibody (14C12 mAb), its paired mouse monoclonal calcitonin antibody (14A2 mAb), CRP-free serum, and PCT-free serum were obtained from Hytest. Analytically pure reagents: cadmium oxide (CdO), selenium powder (Se), powdered sulfur (S), oleic acid (OA), zinc oxide powder (ZnO), octadecene (ODE), octadecyl amine (ODA), trioctylphosphine oxide (TOPO), sodium azide were obtained from Sigma-Aldrich; mercaptoacetic acid (MAA), trichloromethane, normal hexane, methanol, acetone, heptane, glycine, sodium tetraborate, and sucrose were from China National Pharmaceutical Group. Standard human CRP and standard human PCT, bovine serum albumin (BSA), and phosphate-buffered saline (PBS) were from Sigma-Aldrich. Guarantee reagents: N-hydroxysuccinimide (NHS) and carbodiimide (EDC) were from China National Pharmaceutical Group. Nitrocellulose membrane and glass cellulose membrane were from Millipore; the whole blood separator was from Shanghai JieYi Biotechnology; and the stickiness baseplate was from Shanghai Liangxin Science and Technology Ltd. Ultrapure water (Milli-Q) is used in the detections.
3
Quantitative Analysis of Herbal Compounds
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HPLC-grade acetonitrile was
purchased from Thermo Fisher Scientific (Fair Lawn, NJ, USA). HPLC-grade
formic acid and 2,3,5-triphenylte trazolium chloride (TTC) were purchased
from Sigma-Aldrich (St.Louis, MO, USA). Sucrose, KH2PO4, KCl, chloral hydrate, and sodium carboxymethyl cellulose
(CMC-Na) were purchased from Sinopharm Chemical Reagent Co., Ltd.
(Beijing, China). Tris-base and the BCA protein assay kit were purchased
from Applygen Technologies Inc. (Beijing, China). EDTA-Na2 and ADP-Na2 were purchased from Beijing Biodee Biotechnology
Co., Ltd. (Beijing, China). ATPase assay kits were purchased from
Nanjing Jiancheng Bioengineering Institute (Nanjing, China), and edaravone
was purchased from Aladdin Biomedical Technologies Co., Ltd. (Shanghai,
China).
Standards, including shanzhiside, gardenoside, genipin-1-β-D-gentiobioside,
geniposide, 6″-O-p-coumaroylgenipin gentiobioside,
geniposidic acid, deacetyl asperulosidic acid methyl ester, scandoside
methyl ester, chlorogenic acid, crocin-I, and crocin-II, were purchased
from Chengdu Croma Biological Co., Ltd. (Chengdu, China). The purity
of all standard components was more than or equal to 98%. Finally,
edaravone was purchased from Aladdin Biomedical Technologies Co.,
Ltd. (Shanghai, China). Samples of GF, GFP, and GFC were from same
batches and processed in Baicaokangshen Pharmaceutical Company (Hebei,
China).
purchased from Thermo Fisher Scientific (Fair Lawn, NJ, USA). HPLC-grade
formic acid and 2,3,5-triphenylte trazolium chloride (TTC) were purchased
from Sigma-Aldrich (St.Louis, MO, USA). Sucrose, KH2PO4, KCl, chloral hydrate, and sodium carboxymethyl cellulose
(CMC-Na) were purchased from Sinopharm Chemical Reagent Co., Ltd.
(Beijing, China). Tris-base and the BCA protein assay kit were purchased
from Applygen Technologies Inc. (Beijing, China). EDTA-Na2 and ADP-Na2 were purchased from Beijing Biodee Biotechnology
Co., Ltd. (Beijing, China). ATPase assay kits were purchased from
Nanjing Jiancheng Bioengineering Institute (Nanjing, China), and edaravone
was purchased from Aladdin Biomedical Technologies Co., Ltd. (Shanghai,
China).
Standards, including shanzhiside, gardenoside, genipin-1-β-D-gentiobioside,
geniposide, 6″-O-p-coumaroylgenipin gentiobioside,
geniposidic acid, deacetyl asperulosidic acid methyl ester, scandoside
methyl ester, chlorogenic acid, crocin-I, and crocin-II, were purchased
from Chengdu Croma Biological Co., Ltd. (Chengdu, China). The purity
of all standard components was more than or equal to 98%. Finally,
edaravone was purchased from Aladdin Biomedical Technologies Co.,
Ltd. (Shanghai, China). Samples of GF, GFP, and GFC were from same
batches and processed in Baicaokangshen Pharmaceutical Company (Hebei,
China).
4
Synthesis of Trilithium Citrate Tetrahydrate
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All reagents were of analytical grade and used as received. The trilithium citrate tetrahydrate and sucrose were purchased from Sinopharm Chemical Reagent Co., Ltd. The sodium hydroxide (NaOH, ≥96%) and hydrochloric acid (HCl, 36–38%) were obtained from Guangzhou Chemical Reagent Factory.
5
Poplar Veneer Preparation Protocol
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Sucrose (analytical grade) and citric acid (analytical grade) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Each reagent was vacuum dried at 60 °C until reaching a constant mass prior to use in experiments. Poplar veneers (species: Populus tomentosa Carr) were purchased from Zuogezhuang, Langfang, China, and the product method was rotary cutting.
6
Rearing Drosophila on Standard Medium
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Flies were reared on the standard medium (per liter of medium: 21.08 g sucrose (Sinopharm Chemical Reagent, Ltd, cat. #10021418), 42.16 g glucose (Sinopharm Chemical Reagent, Ltd, cat. #63005518), 20.79 g yeast powder (Angel), 7.07 g agar (BioFroxx, cat. #1182GR500), 51.8 g cornmeal flour, 0.484 g CaCl2, 1.33 g of potassium sorbate (Aladdin, cat. #P103845)) and maintained at 25 °C, 50–60% relative humidity, 12-h light: dark cycle. Flies were transferred to new vials with fresh medium every two days.
7
Xinli No. 7 Fruit Phytochemical Analysis
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Xinli No. 7 (XL7) specimens were purchased from a supermarket in Weixian, Hebei Province when they reached the maturity of the respective commercial harvesting period and immediately stored at 4 °C after sampling for subsequent processing analysis. The fruits with diseases, mechanical damage, and immaturity were removed. Hydrochloric acid, sulfuric acid, methanol, anhydrous ethanol, ethyl acetate, zinc acetate, sodium carbonate, sodium bicarbonate, sodium nitrite, aluminium nitrate, sodium chloride, sodium hydroxide (analytically pure), sucrose, potassium ferricyanide, phenolphthalein, polyethylene glycol (PEG 6000, analytically pure), acetic acid–sodium acetate buffer solution (0.1 mol/L, pH = 5.5), Triton X–100, catechol, and potassium persulfate were purchased from Sinopharm Chemical Reagent Co., Ltd. (Beijing, China). Folinol (1 mol/L), gallic acid, rutin, 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH), and 2,2′-diazo-di-3-ethylbenzothiazolin-6-sulfonic acid (ABTS) were bought from Soleibao Biotechnology Co., Ltd. (Shanghai, China).
8
Tissue Fixation and Permeabilization
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Dulbecco's phosphate buffered saline (PBS, Gibco), Sucrose (Sinopharm Chemical Reagent Co., Ltd), Triton X-100 (Aladdin), Paraformaldehyde (Shanghai Lingfeng Chemical Reagent Co., Ltd). All of the reagents were dissolved in PBS.
9
Quantitative Analysis of Tobacco Alkaloids
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The methanol was chromatographic grade and purchased from Fisher Scientific (Hampton, NH). Water (18.2 MΩ-cm) was produced by a Millipore Milli-Q system (Burlington, MA). Ammonium acetate was chromatographic grade and purchased from Fluka (Buchs, Switzerland). Formic acid was chromatographic grade and purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). The nicotine (99.7%) came from the Dr. Ehrenstrofer GmbH reagent company (Augsburg, Germany). The anatabine (97%), nornicotine (98%), and anabasine (94%) were purchased from J&K (Beijing, China). The chloroform, anhydrous sodium sulphate, sodium hydroxide, sulphuric acid, and sucrose were analytical grade and purchased from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China).
The four alkaloid (nicotine, anatabine, nornicotine, and anabasine) standards were diluted into 100 μg/mL stock solution with a 50% (v:v) methanol aqueous solution in four 100 mL brown flasks for the preparation of the standard solutions. The stock solution was stored in a refrigerator at a temperature of 4 °C.
The four alkaloid (nicotine, anatabine, nornicotine, and anabasine) standards were diluted into 100 μg/mL stock solution with a 50% (v:v) methanol aqueous solution in four 100 mL brown flasks for the preparation of the standard solutions. The stock solution was stored in a refrigerator at a temperature of 4 °C.
10
Poplar Veneer Sucrose Extraction
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Sucrose (analytical-grade reagent) and ammonium dihydrogen phosphate (analytical-grade reagent) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Each reagent was vacuum-dried at 60 °C until constant mass prior to usage in experiments. Poplar veneers were obtained from Zuogezhuang, Hebei Province of China.
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