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42 protocols using β cyclodextrin β cd

1

Formulation and Evaluation of Selenite Nanoparticles

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Sodium selenite, indomethacin, β-cyclodextrin (β-CD), Pluronic® F127 (PF-127), soybean lecithin, bovine serum albumin (BSA), carrageenan and glutathione (GSH) were purchased from Sigma (St Louis, MO, USA). Polyethylene glycol (PEG 6000) was purchased from Adwic, EL-Nasr Pharmaceutical Chemicals (Cairo, Egypt). All other chemicals and solvents used were of analytical grade.
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2

Imazalil Encapsulation in Cucurbit[8]uril and Beta-Cyclodextrin

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Imazalil (IMZ), cucurbit[8]uril (CB8), and β-cyclodextrin (β-CD) were purchased from Sigma-Aldrich Chemie GmbH, Taufkirchen, Germany (purity 99%). D2O, DCl and NaOD were also purchased from Sigma-Aldrich. Millipore water had conductivity less than 0.05 μS. The pH values of the solutions were adjusted (±0.2 units) by adding adequate amounts of HCl (DCl) or NaOH (NaOD) and were recorded using a pH meter (WTW 330i equipped with a WTW SenTix Mic glass electrode).
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3

Analytical Method Development for Paxlovid

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Pure reference standard of nirmatrelvir (99.36%) and ritonavir (99.62%) were kindly supplied by Pfizer, Inc, Egypt. The purity was checked by applying the reported method [13 ].

Paxlovid tablets, pink oval nirmatrelvir film-coated tablet (150 mg) co-packaged with white ritonavir film-coated tablet (100 mg), (B. NO: A1324, manufactured by Pfizer Company), were kindly supplied by Pfizer, Inc., Egypt. The recommended dose is two tablets of nirmatrelvir plus one tablet of ritonavir.

Acetonitrile, chloroform, ethanol and methanol (Sigma-Aldrich, Germany).

Tween 80, Sodium dodecyl sulphate “SDS”, El-Nasr Company, Egypt. Cetyl trimethyl ammonium bromide “CTAB” (Win lab, UK). β-cyclodextrin “β-CD”, Sigma-Aldrich, Germany.

Hydrochloric acid and Sodium hydroxide, El-Nasr Company, Egypt. 0.1 N aqueous solutions were prepared.

Boric acid, glacial acetic acid, potassium chloride acid and sodium acetate trihydrate, El-Nasr Company, Egypt.

Acetate buffer solutions in the pH range 4 to 6 and alkaline borate buffer in the pH range 8 to 10.

Drug-free human plasma of healthy volunteers was supplied by Blood Bank, Al-Azhar University Hospital, Damietta, Egypt.

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4

Characterization of Cellulose Nanofibers

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Cellulose from fir pulp was kindly provided by Bartoli SPA (Carraia (Lu), Italy). Racemic ibuprofen as sodium salt and β-cyclodextrin (β-CD) were purchased from Sigma-Aldrich (St. Louis, MO, USA). All other chemicals were commercially available and were used without further purification. The morphological characterization of TOUS-CNFs was obtained by a transmission electron microscope (TEM, Philips CM 200, Koninklijke Philips N.V., Amsterdam, Netherlands) operating at 200 kV and equipped with a field emission gun filament.
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5

Synthesis and Functionalization of Fluorescent Probes

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1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC × HCl), 4 dimethylaminopyridine (DMAP), dimethyl sulfoxide (DMSO), folic acid (FA), formic acid, penicillin, streptomycin, and β-cyclodextrin (β-CD) were all purchased from Sigma-Aldrich, St. Louis, MO, USA. LysoTracker Red DND-99 and Alexa Fluor® 647 phalloidin were purchased from Invitrogen, Eugene, Oregon, USA. Methanol (gradient grade quality for HPLC) was obtained from Merck KGaA, Darmstadt, Germany. All used reagents and chemicals were of analytical grade and were used as received without any further purification. The synthesis method of C-2028 compound 9′-{N[(imidazo[4,5,1-de]-acridin-6-on-5-yl)aminopropyl]-N-methylaminopropylamino}-1′-nitroacridine and QDs (QDgreen and QDred) were as previously described [22 (link)]. Ultrapure water (conductivity 0.056 μS·cm−1) was used for the preparation of all aqueous solutions (Milli-Q® IQ 7005 Water Purification System, Merck KGaA, Germany).
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6

Griseofulvin Cyclodextrin Formulation

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Griseofulvin (GRI), β -Cyclodextrin (β-CD) and Diphenyl Carbonate (DPC) were purchased from Sigma Al-drich (St. Louis, USA). Poly vinyl pyrolidone K30 was obtained from Acros Organics (Belgium). Ferric Chloride was obtained from Alpha Chemika (Mumbai, India). Ethanol (LC-MS grade), Acetonitrile, Formic acid, Methanol and Propranolol hydrochloride were purchased from Sigma Aldrich (Chemie GmbH, Steinheim, Germany). Ultra-pure grade water used throughout the study was prepared from deionized water, PURELAB Prima, VWS Ltd. (ELGA- Lab Water Global Operation Centre, High Wycombe, HP14 3BY, Bucks, UK). All other chemicals and solvents were commercially available analytical grade materials.
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7

Extraction and Characterization of Açai Oil

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The Euterpe oleracea Martius (açai) oil was obtained from Amazon Oil (Belém, Brazil), which reports that the açai fruits were collected in the Brazilian Amazon rainforest. The EOO, obtained from the fruit pulp, was extracted by a cold-pressing method without the addition of solvents or preservatives. β-cyclodextrin (βCD) and hydroxypropyl-β-cyclodextrin (HPβCD) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Inclusion complexes were previously prepared by the kneading and slurry methods and characterized as previously described [16 (link)]. All the experiments were performed using purified water (<1.3 μS) obtained by reverse osmosis, and the reagents were of analytical grade.
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8

Quercetin-Loaded Cyclodextrin Formulation

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β-Cyclodextrin (β-CD), potassium hydroxide (KOH), quercetin (QRC) (>99.5% purity), methanol (MeOH), and dichloromethane (CH2Cl2) of analytical grade were purchased from Sigma-Aldrich Co. Ltd., Seoul, Republic of Korea. Deionized water was prepared in our laboratory.
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9

Membrane Protein Reconstitution Protocol

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Potassium
chloride (KCl), sodium
phosphate dibasic dihydrate (Na2HPO4·2H2O), sodium phosphate monobasic dihydrate (NaH2PO4·2H2O), β-cyclodextrin (βCD),
and heptakis(6-O-sulfo)-β-cyclodextrin heptasodium salt (S7βCD) were all purchased from Sigma (St. Louis, MO) and
used as received. 10.0 mM phosphate buffer (pH = 7.4) was prepared
by dissolving the appropriate amounts of the dibasic and monobasic
phosphate salts in ultrapure water (Milli-Q ultrapure water purification
system). 1,2-Diphytanoyl-sn-glycero-3-phosphocholine
(DPhPC) was obtained from Avanti Polar Lipids (Alabaster, AL) and
stored at −20 °C until use. α-Hemolysin lyophilized
powder was purchased from Sigma and used as received without further
purification.
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10

Curcumin and β-Cyclodextrin Purification

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All chemicals were used without further purifications. β-cyclodextrin (β-CD) and curcumin (Cur) were purchased from Sigma-Aldrich (Saint-Quentin Fallavier, France). All reactions involving Cur compounds were protected from the light by using aluminum foil around the reactors and other glassware. Deionized (DI) water was processed in a Milli-Q water purification system.
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