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Dulbecco s modified eagle s medium high glucose without l glutamine

Manufactured by Merck Group
Sourced in United States, Australia

Dulbecco's Modified Eagle's Medium—high glucose without L-Glutamine is a cell culture medium commonly used for the in vitro maintenance and growth of various cell lines. It provides essential nutrients, vitamins, and salts required for cell proliferation, while excluding the amino acid L-Glutamine, which must be supplemented separately.

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2 protocols using dulbecco s modified eagle s medium high glucose without l glutamine

1

Targeted Photodynamic Therapy for PDAC

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Resomer® RG 504 H; Poly(D,L-lactide-co-glycolide) (PLGA; #719900), poly(vinyl alcohol) (PVA; #363138), dichloromethane (DCM; #650463), Verteporfin (VP; #SML0534), dimethyl sulfoxide (DMSO; #D2650), 1-[3-(Dimethylamino)propyl]-3-ethylcarbodiimide methiodide (EDC; #165344), N-Hydroxysuccinimide (NHS; #130672), human pancreatic ductal adenocarcinoma cell line (PANC-1), Dulbecco’s Modified Eagle’s Medium—high glucose without L-Glutamine (#D5671) and Gentian violet (#G2039) were purchased from Sigma-Aldrich (St. Louis, MI, USA). Singlet oxygen sensor green probe (SOSG; #S-36002), NucBlue™ Live ReadyProbes™ Reagent (#R37605), LIVE/DEAD™ Viability/Cytotoxicity Kit for mammalian cells (#L3224), HCS DNA Damage Kit (H10292), Image-iT™ Lipid Peroxidation Kit for live cell analysis (C10445), DPBS with calcium and magnesium (#14040182), DPBS with no calcium or magnesium (#14190250), TrypLE™ Express Enzyme (1X) with no phenol red (#12604021), L-Glutamine, 200 mM (#25030081) and Fetal Bovine Serum (FBS) qualified US origin (#26140079) were purchased from Life Technologies (Carlsbad, CA, USA). Apoptosis/Necrosis Assay Kits (blue, red, green) (ab176750) and CellTiter 96® AQueous One Solution Assay were purchased from Abcam (Cambridge, UK) and Promega (Madison, WI, USA) respectively. Except as otherwise noted, all commercial assays were performed according to the manufacturers’ instructions.
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2

PLGA Nanoparticles for Photodynamic Therapy

Check if the same lab product or an alternative is used in the 5 most similar protocols
Resomer® RG 504 H; Poly(D,L-lactide-co-glycolide) (PLGA; #719900), poly(vinyl alcohol) (PVA; #363138), dichloromethane (DCM; #650463), Verteporfin (VP; #SML0534), perfluorooctylbromide (PFOB, or 1-Bromoheptadecafluorooctane; #343862), acetone (#179124), dimethyl sulfoxide (DMSO; # D2650), human PDAC cell line (PANC-1), Dulbecco’s Modified Eagle’s Medium-high glucose without L-Glutamine (#D5671), Gentian violet (#G2039) were purchased from Sigma-Aldrich, North Ryde, NSW, Australia. Singlet Oxygen Sensor Green probe (SOSG; #S-36002), NucBlue™ Live ReadyProbes™ Reagent (#R37605), LIVE/DEAD™ Viability/Cytotoxicity Kit for mammalian cells (#L3224), MitoTracker™ Green FM (#M7514), LysoTracker™ Green DND-26 (#L7526), DPBS, with calcium, magnesium (#14040182), DPBS, with no calcium, no magnesium (#14190250), TrypLE™ Express Enzyme, with no phenol red (#12604021), L-Glutamine, 200 mM (#25030081) and Fetal Bovine Serum, qualified, US origin (#26140079) were purchased from Life Technologies, Mulgrave, VIC, Australia. CellTiter 96® AQueous One Solution Assay was purchased from Promega, Sydney, NSW, Australia.
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