Uv 240

Total cholesterol (TC), triglyceride (TG), low-density lipoprotein (LDL), high-density lipoprotein (HDL)-cholesterol levels, urea, and creatinine were measured in serum at 546 nm using commercial diagnostic kits by spectrophotometer model UV-240, Shimadzu (Burladingen, Germany).

Quantification of SOD activity was based on the inhibition of the reduction of cytochrome c by the superoxide anion [52 (link)]. Cytochrome c reduction was monitored by measuring the absorbance at 550 nm (UV-240, Shimadzu, Kyoto, Japan). One unit of SOD activity is defined as the amount of enzyme required to inhibit the cytochrome c reduction by 50% per min. SOD specific activity was expressed in SOD activity units per mg of total protein. SOD activity was also monitored in native-PAGE gels by a negative specific staining [52 (link)]. Electrophoresis was performed on 10% (w/v) native-PAGE gels that were subsequently incubated in a 2.5 mM nitroblue tetrazolium (NBT) solution in 36 mM potassium-phosphate buffer pH 7.8 for 20 min in the dark. Afterwards, gels were soaked in 86 μM riboflavin and 28 mM tetramethylethylenediamine (TEMED) in 36 mM potassium phosphate buffer pH 7.8 for 20 min. Finally, gels were exposed to incandescent lights until the colourless bands, indicative of SOD activity, were visible in a blue background.

AMX (20 mg) and Zn-MOFs (10 mg) were dispersed in distilled water and stirred for 24 h at 200 rpm. After that, the mixture was centrifuged at 10,000 rpm for 15 min, and the resulting AMX-loaded Zn-MOFs (Am-Zn-MOFs) were washed and dried. The pallet was then dispersed in ethanol and sonicated for 30 min, and the undissolved Zn-MOFs were separated by centrifugation at 10,000 rpm for 20 min. The drug content in the supernatant was studied using a UV-Vis spectrophotometer (UV-240, Shimadzu, Japan) and the percent drug encapsulation (DE) was determined according to the Equation (1) [50 (link)].
%DEAmount of drug in Zn−MOFsTotal amount of drug used×100

Colon tissues (100 mg) were homogenized in hexadecyltrimethylammonium bromide (0.5%) plus 50 mM of potassium phosphate buffer (pH 6). They were sonicated in an ice bath for 10 s and then cooled three times in ice, with each repeated sonication. The homogenized samples were centrifuged for 10 min at 8000 rpm. 0.1 mL of the sample was mixed with 2.9 mL of 50 mM of phosphate buffer (pH 6.0) containing 0.167 mg/mL o-dianisidine dihydrochloride and 0.0005% hydrogen peroxide. MPO activity was measured with a spectrophotometer at 460 nm for 5 min (Shimadzu model UV-240).

Total phenolic content in oil was determined using Folin-Ciocalteu colorimetric method by UV-visible spectrophotometer using Shimadzu, UV-spectrophotometer UV-240 [46 (link)].