Sodium chloride (NaCl, 99%), potassium chloride (KCl, >99%), sodium hydrogen carbonate (NaHCO3, >99%), calcium chloride (CaCl2, >98%), hydrochloric acid (HCl, 37%), ammonium chloride (NH4Cl), sodium dihydrogen phosphate monohydrate (NaH2PO4·H2O, >99%), potassium dihydrogen phosphate (KH2PO4), magnesium chloride (MgCl2, >99%), urea (≥99%), potassium persulfate (K2S2O8, >99%), potassium ferricyanide, and trichloroacetic acid (TCA) were obtained from Shanghai Macklin Biochemical Co. Ltd. (Shanghai, China). Folin–Ciocalteu reagent, pepsin (from porcine gastric mucosa), mucin (from the porcine stomach), pancreatin (from the porcine pancreas), lipase (from the porcine pancreas), bile extract porcine, 2-diphenyl-1-picrylhydrazyl (DPPH, 95%), and 2,2′-azinobis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS, >98%) were obtained from Sigma-Aldrich.
Potassium ferricyanide
Manufactured by Maclin Biochemical Technology
Sourced in China
Potassium ferricyanide is a chemical compound with the formula K3[Fe(CN)6]. It is a stable, crystalline solid that is commonly used as a redox indicator and in various analytical and industrial applications.
Automatically generated - may contain errors
Lab products found in correlation
Ferric chloride, by Maclin Biochemical Technology (6 mentions)
Trichloroacetic acid, by Maclin Biochemical Technology (6 mentions)
Potassium persulfate, by Maclin Biochemical Technology (4 mentions)
Hydrochloric acid (hcl), by Maclin Biochemical Technology (3 mentions)
Sodium chloride (nacl), by Maclin Biochemical Technology (2 mentions)
Sodium hydroxide (naoh), by Maclin Biochemical Technology (2 mentions)
Ethanol, by Maclin Biochemical Technology (2 mentions)
Sodium carbonate, by Maclin Biochemical Technology (2 mentions)
Potassium ferrocyanide, by Maclin Biochemical Technology (2 mentions)
Formic acid, by Merck Group (2 mentions)
2 2 azino bis, by Merck Group (1 mentions)
Porcine bile extract, by Merck Group (1 mentions)
1 diphenyl 2 picrylhydrazyl, by Merck Group (1 mentions)
Folin ciocalteu reagent, by Merck Group (1 mentions)
Potassium chloride (kcl), by Maclin Biochemical Technology (1 mentions)
Pepsin, by Merck Group (1 mentions)
Pancreatin, by Merck Group (1 mentions)
Potassium dihydrogen phosphate, by Maclin Biochemical Technology (1 mentions)
Sodium hydrogen carbonate, by Maclin Biochemical Technology (1 mentions)
Lipase, by Merck Group (1 mentions)
14 protocols using potassium ferricyanide
1
In Vitro Digestion and Antioxidant Assays
2
Enzyme-Based Nanocomposite Glucose Sensor
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Glucose oxidase (GOx, from Aspergillus niger), chitosan, single-walled carbon nanotubes, iron(III) chloride, potassium ferricyanide(III), and 5% Nafion solution were purchased from Macklin. Acetic acid (2%), potassium chloride, and PBS (pH 7.2) were purchased from Aladdin, and 100 μm thick PI film was purchased from McMaster-Carr. All reagents were used as received.
3
Antioxidant Evaluation and Cytotoxicity Assay
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NaOH, AlCl3, NaNO2, 2,2′-Diphenyl-1-picrylhydrazyl (DPPH), gallic acid, Na2CO3, 2,2′-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS), potassium persulfate, potassium ferricyanide, ferric chloride, and trichloroacetic acid were obtained from Shanghai Macklin Biochemical Technology Co., Ltd. (Shanghai, China) Rutin, methyl thiazolyl tetrazolium (MTT) and Vitamin C (Vc) were purchased from Shanghai Yuanye Biotechnology Co., Ltd. (Shanghai, China), while foline-phenol, β-sitosterol, and vanillin were purchased from Sigma-Aldrich Corporation (St. Louis, MI, USA). Assay kits for LDH, MDA, SOD, and GSH-PX were bought from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). RPMI-1640 culture medium, phosphate buffer solution (0.01 M, pH 7.2), dimethyl sulfoxide (DMSO), and 0.05%Trypsin-EDTA were purchased from Gibco (Grand Island, NY, USA), while fetal bovine serum (FBS) was purchased from Zhejiang Tianhang Biotechnology Co., Ltd. (Huzhou, China). ROS assay kit and 30% H2O2 solution were gained from Beijing Solarbio Technology Co., Ltd. (Beijing, China). All organic reagents used in the experiments, including petroleum ether (bp. 60–90 °C), ethyl acetate, n-butanol, and 95% ethanol, were of analytical grade.
4
Antioxidant Assays: TPTZ, DPPH, ABTS
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TPTZ (tripyridyltriazine), DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS (2, 2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) were purchased from Sigma-Aldrich (Darmstadt, Germany). Phosphoric acid, methanol-D4, chloroform-d, DMSO (Dimethyl Sulfoxide)-D6 were purchased from Aladdin. Analytical-grade methanol, ethanol, petroleum ether (PE), ethyl acetate (EA), chloroform and n-butanol (n-Bu), in addition to HPLC-grade acetonitrile, methanol were purchased from Shanghai Macklin Biochemical Co, Ltd. Potassium ferricyanide, trichloroacetic acid, ferric chloride, potassium persulfate, hydrochloric acid were of analytical grades.
5
Surimi-Based Antioxidant Evaluation
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Frozen silver carp surimi was purchased from Fujian Anjing Foods Co., Ltd. (Xiamen, China), and kept at −20 °C until used. Black tea powder (caffeine removed) was purchased from Damin Foodstuff (Zhangzhou) Co., Ltd. (Zhangzhou, China). Tapioca starch, phosphate, salt and glutamine transaminase (TG enzyme) were commercially available.
1,1-diphenyl-2-picrylhydrazyl (DPPH) was purchased from Shanghai Yuanye Biotechnology Co., Ltd. (Shanghai, China); 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) was purchased from Beijing Solarbio Technology Co., Ltd. (Beijing, China); sodium dihydrogen phosphate, dibasic sodium phosphate, trichloroacetic acid, hydrogen peroxide, ferrous sulfate and potassium persulfate were of analytical grade and purchased from Xilong Chemical Co., Ltd. (Guangzhou, China). Absolute ethanol was purchased from Sinopharm Group Chemical Reagent Co., Ltd. (Shanghai, China); ferric chloride, potassium ferricyanide, and 3,5-Dinitrosalicylic acid were purchased from Shanghai Macklin Biochemical Technology Co., Ltd. (Shanghai, China).
1,1-diphenyl-2-picrylhydrazyl (DPPH) was purchased from Shanghai Yuanye Biotechnology Co., Ltd. (Shanghai, China); 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) was purchased from Beijing Solarbio Technology Co., Ltd. (Beijing, China); sodium dihydrogen phosphate, dibasic sodium phosphate, trichloroacetic acid, hydrogen peroxide, ferrous sulfate and potassium persulfate were of analytical grade and purchased from Xilong Chemical Co., Ltd. (Guangzhou, China). Absolute ethanol was purchased from Sinopharm Group Chemical Reagent Co., Ltd. (Shanghai, China); ferric chloride, potassium ferricyanide, and 3,5-Dinitrosalicylic acid were purchased from Shanghai Macklin Biochemical Technology Co., Ltd. (Shanghai, China).
6
Antioxidant Capacity of Pullulan-Based Composites
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Bovine serum albumin (BSA), epigallocatechin gallate (EGCG), anhydrous ferric chloride, potassium ferricyanide, ferrous ammonium sulfate hexahydrate, salicylic acid, trichloroacetic acid, 1,1-diphenyl-2-Picrylhydrazine (DPPH), and 2,2′-Diaza-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) were obtained from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). Pullulan (PUL) was purchased from Shanghai Yien Chemical Technology Co., Ltd. (Shanghai, China). Hydrogen peroxide (purity ≥ 30%), potassium dihydrogen phosphate, dipotassium hydrogen phosphate, absolute ethanol, hydrochloric acid, and sodium hydroxide were purchased from China National Medicines Corporation Ltd. (Shanghai, China).
7
Cathode and Anode Electrolyte Preparation
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For the cathode electrolyte, 0.5 g of potassium ferricyanide (Macklin biochemical Co., Ltd, China, Shanghai) was dissolved in 100 ml of phosphate buffer solution (PBS) with a pH of 6.5 to obtain a 5 g/L solution. The anode electrolyte contained basal medium (7.5 g/L NH4SO4 (Macklin biochemical Co., Ltd, China, Shanghai), 14.4 g/L KH2PO4 (Aladdin Biochemical Technology Co., Ltd, China, Shanghai), 0.5 g/L MgSO4·7H2O (Sinopharm Chemical Reagent Co., Ltd, China, Beijing), 0.1% vitamin solution, and 0.1% trace metal solution [62 (link)]). The anode electrolyte was adjusted to a pH of 6.5 with KOH and autoclaved at 116 °C for 25 min.
8
Triboelectric Charging of Liquid Droplets
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Redistilled solvents and Milli-Q water (>18
MΩ cm) were used for substrate cleaning and preparation of solutions.
An FEP (30 μm, DAIKIN) film was used for triboelectrification
with liquid droplets. Sodium chloride (99.5%), sodium iodide (99.5%),
sodium nitrate (99%), sodium bicarbonate (99.5%), sodium carbonate
(99.5%), sodium sulfite (98%), sodium sulfate (99%), magnesium sulfate
(99%), potassium chloride (99.5%), potassium ferricyanide (99.5%),
calcium chloride (97%), chromic nitrate (99%), manganese(II) chloride
(99%), manganese(II) sulfate (99%), ferric nitrate (98.5%), nickel(II)
chloride (99%), copper sulfate (99%), copper(II) nitrate (99%), zinc
nitrate (99%), zinc acetate (98%), hydrochloric acid (37%), sulfuric
acid (98%), nitric acid (68%), potassium hydroxide (99.9%), and sodium
hydroxide (97%) were purchased from Macklin. Ethanol (99.7%) and acetone
(99.5%) were obtained from Yong Da Chemical.
MΩ cm) were used for substrate cleaning and preparation of solutions.
An FEP (30 μm, DAIKIN) film was used for triboelectrification
with liquid droplets. Sodium chloride (99.5%), sodium iodide (99.5%),
sodium nitrate (99%), sodium bicarbonate (99.5%), sodium carbonate
(99.5%), sodium sulfite (98%), sodium sulfate (99%), magnesium sulfate
(99%), potassium chloride (99.5%), potassium ferricyanide (99.5%),
calcium chloride (97%), chromic nitrate (99%), manganese(II) chloride
(99%), manganese(II) sulfate (99%), ferric nitrate (98.5%), nickel(II)
chloride (99%), copper sulfate (99%), copper(II) nitrate (99%), zinc
nitrate (99%), zinc acetate (98%), hydrochloric acid (37%), sulfuric
acid (98%), nitric acid (68%), potassium hydroxide (99.9%), and sodium
hydroxide (97%) were purchased from Macklin. Ethanol (99.7%) and acetone
(99.5%) were obtained from Yong Da Chemical.
9
Antioxidant Activities of Natural Polyphenols
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Six natural polyphenols (quercetin, caffeic acid, sinapic acid, catechin, ferulic acid, 3,4-dihydroxybenzoic acid), ascorbic acid, HPLC-grade methanol, as well as potassium persulfate, trichloroacetic acid, ferric chloride, and potassium ferricyanide were obtained from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). While DPPH, ABTS, and SAC were supplied by Shanghai yuanye Bio-Technology Co., Ltd. (Shanghai, China).
10
Electrochemical Detection of Aflatoxins
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Potassium ferricyanide and potassium ferrocyanide were obtained from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). We obtained rGO, chloroauric acid, triclofos sodium, and 6-mercaptoethanol (MCH) from Shanghai Aladdin Bio-Chem Technology Co., Ltd (Shanghai, China). The sequences (Table 1) were purchased from Sangon Biotech Co., Ltd. (Shanghai, China). We obtained AFM1, aflatoxin M2, aflatoxin B1, aflatoxin G1 and aflatoxin G2 (>99%) from Pribolab Pte. Ltd. (Singapore). The sample of pure milk was purchased from Yonghui Supermarket (Chongqing, China).
All electrochemical measurements were conducted on the CHI600E workstation (Shanghai CH Instruments, China). The 3-electrode cell was used, where the GCE was chosen as the working electrode, the saturated calomel electrode as the reference electrode, and the platinum wire as the counter-electrode. Scanning electron microscopy was conducted using a Gemini SEM 300 scanning electron microscope (Carl Zeiss AG, Germany). Deionized water was purified using a Millipore (Bedford, MA) Milli-Q Ultrapure Water System and used for all experiments.
All electrochemical measurements were conducted on the CHI600E workstation (Shanghai CH Instruments, China). The 3-electrode cell was used, where the GCE was chosen as the working electrode, the saturated calomel electrode as the reference electrode, and the platinum wire as the counter-electrode. Scanning electron microscopy was conducted using a Gemini SEM 300 scanning electron microscope (Carl Zeiss AG, Germany). Deionized water was purified using a Millipore (Bedford, MA) Milli-Q Ultrapure Water System and used for all experiments.
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