Illustrator cs2

The two type specimens, both with part and counterpart, were collected from a finely laminated tuff in the latest Middle Jurassic Jiulongshan Formation at Daohugou Village, Ningcheng County, Inner Mongolia, China. The Daohugou locality is now considered to be one of the most important insect Lagerstätten31 32 . Because of new calibrations for the Jurassic System, this deposit should be now considered as latest Middle Jurassic (late Callovian) in age9 , approximately 165 Mya - 164 Mya.
The specimens studied in this paper were examined and then photographed, either dry or wetted with 95% ethanol, with a Leica DFC450 digital camera attached to a Leica M250 C dissecting microscope (Leica, Wetzlar, Germany). The line drawings were prepared using Adobe Illustrator CS2 and Adobe Photoshop CS5 software. The wing venation nomenclature used in this article is modified after Rasnitsyn1 2 . The type materials described are deposited in the Key Lab of Insect Evolution and Environmental Changes, College of Life Sciences, Capital Normal University, in Beijing, China (CNUB; Dong Ren, Curator). Recent species were examined under the dissecting microscopes and kept in the Museum of Forest Biodiversity, Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, in Beijing, China (CAF).

The head capsule and stylets of all individuals were outlined through vector-drawing programs (Inkscape 1.1, Adobe Illustrator CS2). The outline of the better-accessible half was drawn. The stylet was artificially rotated to be straight; the innermost distal point and the innermost proximal point were oriented to form a line parallel to the anterior–posterior midline [79 (link)]. Then, the half object was mirrored.

The specimen was documented with composite imaging under different white light conditions, as well as under autofluorescence. The white-light microscopic images were recorded with a Keyence VHX-6000 equipped with a 20–2000 × objective, either under ring illumination or under coaxial cross-polarised illumination. Black and white background colour was used. To achieve an optimal result, every image was recorded with different exposure times (HDR) [18 (link), 21 (link)]. For autofluorescence images, a Keyence BZ-9000 was used [22 , 23 (link)].
Each image detail was documented as a stack, with the single images of the stack (frames) being recorded in different focal levels in z-axis to overcome limitations in depth of field. The frames of each stack were fused to achieve an entirely sharp image. Several adjacent stacks were recorded in x-y axis to overcome limitations in field of view. All image details were stitched to a final panorama image [24 , 25 (link)].
Additionally, based on the stacks the three-dimensional relief information was extracted (virtual surface). This information is presented as red-cyan stereo anaglyphs [26 (link)].
Drawings of the specimen and of comparative material were prepared in Adobe Illustrator CS2.

We re-used parts of data of the head and stylet shape from Haug et al.^{19 (link)}. Specimens included are extant larvae of the groups Berothidae and Dilaridae as well as fossil larvae potentially representing the same two groups. Additionally, of two specimens depicted in Badano et al.^{58 (link)} the outlines of the head capsule and stylets were redrawn in Adobe Illustrator CS2. Finally, of one of the newly reported larvae the outline of head capsule and stylets were also redrawn and included into the data set. All these outline drawings were transformed into bitmap files and included into an Elliptic Fourier Analysis (EFA) performed with the software SHAPE^{81 (link)}, followed by a principal component analysis (PCA) (for details, see^{19 (link),82 (link)}).

Live cell imaging was performed as described before (77 (link)) using an inverted Axiovert 200 fluorescence microscope (Zeiss), equipped with 100×/1.4 Plan-Apochromat oil-immersion objective (Zeiss), pulsed excitation module (470 nm, 390 nm LEDs), bandpass filters 510–560 nm (EGFP) and 417–477 nm (Hoechst 33342) and gated CCD camera (LaVision BioTec). Briefly, HeLa cells were seeded onto 8-well chambers precoated with a mixture of collagen IV and poly-d-lysine (Ibidi), allowed to attach (24 h) and forward transfected for 24 h with plasmids encoding GFP-VDR fusions as indicated. Prior to live imaging, cells were counterstained with Hoecsht 33342 (1 μm, 30 min). Fluorescence images were collected before (resting) and after stimulation with calcitriol (10⁻⁷m, 10 min, 37 °C). Images were exported using ImSpector software (LaVision BioTec) and combined in Adobe Illustrator CS2.