B6 129p2 pvalbtm1 cre arbr j

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B6;129P2-Pvalbtm1(cre)Arbr/J is a transgenic mouse strain that expresses Cre recombinase under the control of the parvalbumin (Pvalb) gene promoter. Parvalbumin is a calcium-binding protein expressed in a subset of GABAergic interneurons in the central nervous system.

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44 protocols using b6 129p2 pvalbtm1 cre arbr j

Tracing Cortical Connectivity in Mice

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Retrograde tracing experiments with Diamidino Yellow (DY), immunostaining for M2 muscarinic acetylcholine receptor (M2), vesicular glutamate transporter 2 (VGluT2) and cytochrome oxidase (CO) histochemistry were performed in 141 male and female mice. 33 C57BL/6J (Jackon Lab) (of which 14 injected with DY, 3 of which successful for this study; 19 used for immunostaining and histochemistry). 88 PV-Cre (B6.129P2-Pvalb^tm1(cre)Arbr/J, Jackson Lab) × Ai9 (B6;129S6-Gt(ROSA)26Sor^{tm9(CAG-tdTomatoHze}/J, Jackson Lab) of which 24 successful for DY labeling in this study; 8 for fluorescence imaging of VGluT2 (Slc17a6^tm2(cre)Lowl/J, Jackson Lab); and 12 for fluorescence imaging of M2 (B6;Cg-Chrm2^tm1.Hze/J, Jackson Lab). All experimental procedures were approved by the institutional Animal Care and Use Committee at Washington University.
For the 27 mice which were successfully injected, the age span is 8–30 weeks, and sex is known for only 2 cases, both females (see Table S6). The others are mostly males, for technical reasons – females being retained for breeding. Sex wasn’t registered because there are no known sex differences in cortical connectivity.

Gămănuţ R., Kennedy H., Toroczkai Z., Ercsey-Ravasz M., Van Essen D., Knoblauch K, & Burkhalter A. (2018). The Mouse Cortical Connectome Characterized by an Ultra Dense Cortical Graph Maintains Specificity by Distinct Connectivity Profiles. Neuron, 97(3), 698-715.e10.

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Transgenic Mouse Lines for Neuronal Imaging

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Experimental procedures involving animals have been approved by the IIT Animal Welfare Body and by the Italian Ministry of Health (authorization # 34/2015-PR and 125/2012-B), in accordance with the National legislation (D.Lgs. 26/2014) and the European legislation (European Directive 2010/63/EU). The mouse lines B6;129S6-Gt(ROSA)26Sor^{tm14(CAG-TdTomato)Hze}/J, id #007908, (otherwise called TdTomato line) and B6;129P2-Pvalb^tm1(cre)Arbr/J, id #008069, (called PV-cre line) were purchased from the Jackson Laboratory (Bar Harbor, USA). The animals were housed in a 12:12 hr light-dark cycle in individually ventilated cages, with access to food and water ad libitum.

Zucca S., Pasquale V., Lagomarsino de Leon Roig P., Panzeri S, & Fellin T. (2019). Thalamic Drive of Cortical Parvalbumin-Positive Interneurons during Down States in Anesthetized Mice. Current Biology, 29(9), 1481-1490.e6.

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Optogenetic and Genetic Manipulation of V1

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All viruses used to locally infect V1 were adeno-associated viruses (AAV). For optogenetic experiments we infected V1 of ~1 month old mice expressing Cre recombinase directed by the parvalbumin promoter (B6;129P2-Pvalb^tm1(cre)Arbr/J – Jackson laboratory, ME, US) or wild-type littermates with AAV5-EF1α-DIO-hChR2(H134R)-eYFP (UNC viral core – generated by Dr. Karl Deisseroth’s laboratory). Using a glass pipette and nanoject system (Drummond scientific, Broomall, PA, US) we delivered 81 nl of virus at each of 3 cortical depths: 600 μm, 450 μm and 300 μm below surface. At each depth 6 injections of 13.5 nl were delivered, each separated by 15 secs, and 5 mins was allowed between re-positioning for depth. For local GRIN1 knockdown, ~1 month old mice GRIN^fl/fl mutant mice (B6.129S4-Grin1^tm2Stl/J – Jackson laboratory) were infected locally in V1 with either AAV8-hSyn-GFP-Cre (knockdown, UNC viral core) or AAV8-hSyn-GFP (control, UNC viral core – generated by Dr Bryan Roth’s laboratory). Again, injections were made at multiple depths. In this case 10 injections of 13.5 nl were made for a total of 135 nl at 4 cortical depths: 600 μm, 450 μm, 300 μm and 150 μm below surface. As before, each injection was separated by 15 secs, and 5 mins was allowed between re-positioning for depth. Mice were allowed 4 weeks recovery for virus expression to peak before experiments were initiated.

Cooke S.F., Komorowski R.W., Kaplan E.S., Gavornik J.P, & Bear M.F. (2015). Visual recognition memory, manifest as long-term habituation, requires synaptic plasticity in V1. Nature neuroscience, 18(2), 262-271.

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Ethical Guidelines for Mouse Experiments

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Animal experiments were approved by the local German ethics committee (Landesuntersuchungsamt Rheinland-Pfalz Koblenz, #23 177-07/G19-1-085). This study is in accordance with the ARRIVE guidelines. Male PVcre mice (B6; 129P2-Pvalbtm1(cre)Arbr/J; The Jackson Laboratory, Bar Harbor, Maine, USA), aged 4–6 weeks were chosen for experiments. Animals were kept in cages in groups of 2–3 and had ad libitum access to food and water. All procedures followed European and German laws (European Communities Council Directive, 86/ 609/ECC).

Yeganeh F., Knauer B., Guimarães Backhaus R., Yang J.W., Stroh A., Luhmann H.J, & Stüttgen M.C. (2022). Effects of optogenetic inhibition of a small fraction of parvalbumin-positive interneurons on the representation of sensory stimuli in mouse barrel cortex. Scientific Reports, 12, 19419.

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PV Interneuron Labeling in Adult Mice

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All experimental procedures were carried out in accordance with institutional animal welfare guidelines, and licensed by the UK Home Office. Experiments were carried out on adult mice (aged >P90), and no systematic randomization was used with respect to region labeled or other conditions. For experiments in which parvalbumin (PV) interneurons were labeled, this was achieved by crossing the B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hom/J and B6;129P2-Pvalbtm1(cre)Arbr/J (Jackson Laboratory, JAX Stock 007914 and 008069, respectively). Mice were housed under normal light conditions (14 h light, 10 h dark) and recordings were made during the light period.

Ranson A., Broom E., Powell A., Chen F., Major G, & Hall J. (2019). Top-Down Suppression of Sensory Cortex in an NMDAR Hypofunction Model of Psychosis. Schizophrenia Bulletin, 45(6), 1349-1357.

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C57BL/6J and PV-IRES-Cre Mouse Experiments

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Experimental procedures involving animals have been approved by the IIT Animal Welfare Body and by the Italian Ministry of Health (authorization # 34/2015-PR and 125/2012-B), in accordance with the National legislation (D.Lgs. 26/2014) and the European legislation (European Directive 2010/63/EU). Experiments were performed on young-adult (4-6 weeks old, either sex) C57BL/6J n = 4 mice (Charles River, Calco, Italy) and PV-IRES-Cre n = 4 mice (B6;129P2-^{Pvalbtm1(cre)Arbr/J}, Jackson Laboratory, Bar Harbor, USA). The animals were housed in a 12:12 hr light-dark cycle in singularly ventilated cages, with access to food and water ad libitum.

Zerlaut Y., Zucca S., Panzeri S, & Fellin T. (2019). The Spectrum of Asynchronous Dynamics in Spiking Networks as a Model for the Diversity of Non-rhythmic Waking States in the Neocortex. Cell Reports, 27(4), 1119-1132.e7.

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Neuronal activity monitoring in mice and rats

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All protocols and procedures were performed according to the Spanish legislation (R.D. 1201/2005 and L.32/2007) and the European Communities Council Directive 2003 (2003/65/CE) for animal research. Experiments were approved by the Ethics Committee of the Instituto Cajal and the Spanish Research Council. A number of recordings in freely moving rats were obtained at the University of Szeged, Hungary, and were approved by the Animal Care Committee of the University of Szeged.
A total of 25 males and females mice were used from wild-type (C57BL/6J, n = 15), PV-Cre (B6;129P2-Pvalbtm1(cre)Arbr/J; Jackson Labs, n = 9), Calb1-Cre (Calb1-2A-dgCre-D; Jackson Labs, n = 3), and Thy1-ChR2-YFP (B6.Cg-Tg(Thy1-COP4/EYFP)18Gfng/J, Jackson Labs; n = 2) lines. We also used 28 wild-type males and females Wistar rats. Animals were maintained in a 12 h light–dark cycle (7 a.m. to 7 p.m.) with access to food and drink ad libitum.

Navas-Olive A., Valero M., Jurado-Parras T., de Salas-Quiroga A., Averkin R.G., Gambino G., Cid E, & de la Prida L.M. (2020). Multimodal determinants of phase-locked dynamics across deep-superficial hippocampal sublayers during theta oscillations. Nature Communications, 11, 2217.

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Optogenetic Stimulation in PV-Cre Mice

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All procedures were approved by the Animal Care and Use Committee at the Massachusetts Eye and Ear Infirmary and followed guidelines established by the National Institutes of Health for the care and use of laboratory animals. Subjects included 13 PV-Cre:Ai32 mice of either sex (a cross between B6;129P2-Pvalbtm1(cre)Arbr/J and Ai32 (RCL-ChR2(H134R)/EYFP), Jackson Laboratory), aged 16 weeks at the time of optetrode implantation.

Resnik J, & Polley D.B. (2017). Fast-spiking GABA circuit dynamics in the auditory cortex predict recovery of sensory processing following peripheral nerve damage. eLife, 6, e21452.

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Cre-Driver Mouse Line Characterization

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All experiments involving living animals were approved by the National Council on Animal Care of the Italian Ministry of Health (authorization # 34/2015-PR and 125/2012-B) and carried out in accordance with the guidelines established by the European Communities Council Directive. The mouse strain Tg(Rbp4-cre)KL100Gsat/Mmcd (otherwise called Rbp4-cre), identification number 031125-UCD, was obtained from the Mutant Mouse Regional Resource Center, a NCRR-NIH funded strain repository, and was donated to the MMRRC by the NINDS-funded GENSAT BAC transgenic project. B6;129S6-Gt(ROSA)26Sor^{tm14(CAG-TdTomato)Hze}/J, id #007908 (otherwise called TdTomato line), B6;129P2-Pvalb^tm1(cre)Arbr/J, id #008069 (PV-cre line), STOCK Sst^{tm2.1(cre)Zjh}/J, id #013044 (SST-cre line) and B6.Cg-Tg(Thy1-COP4/EYFP)18Gfng/J line 18, id #007612 (Thy1-ChR2 line) were purchased from the Jackson Laboratory (Bar Harbor, USA). All data were collected from mice of either sex. From postnatal days 30, animals were separated from the original cage and housed in group of up to four littermates per cage with ad libitum access to food and water in a 12:12 light-dark cycle. Age of animals used for each experimental dataset is specified in Method Details. The number of animals used for each experimental dataset is specified in the text or in the corresponding Figure legend.

Vecchia D., Beltramo R., Vallone F., Chéreau R., Forli A., Molano-Mazón M., Bawa T., Binini N., Moretti C., Holtmaat A., Panzeri S, & Fellin T. (2020). Temporal Sharpening of Sensory Responses by Layer V in the Mouse Primary Somatosensory Cortex. Current Biology, 30(9), 1589-1599.e10.

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Parvalbumin-Expressing Neuron Optogenetics

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All procedures were approved by the Committee on Animal Care at MIT, Cambridge, MA, USA and accorded with the guidelines of the National Institutes of Health. Mice were male C57BL/6 mice (Charles River laboratory international, Wilmington, MA) aged from P30–45. For optogenetic experiments mice expressing Cre recombinase directed by the parvalbumin promoter were used (B6;129P2-Pvalb^tm1(cre)Arbr/J – Jackson laboratory, ME, US. For local NMDAR knockdown experiments GRIN^fl/fl mutant mice were used (B6.129S4-Grin1^tm2Stl/J – Jackson laboratory). In all cases mice were housed in groups of 2–5 with food and water available ad libitum and maintained on a 12 hour light-dark cycle. All mice participated only in the individual experiment described and did not undergo any prior or future experimental treatment or procedure.

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