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6 0 silk suture

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The 6-0 silk suture is a sterile, non-absorbable surgical suture material manufactured by Johnson & Johnson. It is composed of natural silk fibers and is commonly used in ophthalmic, cardiovascular, and other delicate surgical procedures where precise suturing is required.

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Lab products found in correlation

Silk 4.0 sutures, by Johnson & Johnson (2 mentions) Avertine, by Merck Group (1 mentions) Isoflurane, by Abbott (1 mentions) Silk suture, by Johnson & Johnson (1 mentions) C57bl 6 mice, by Jackson ImmunoResearch (1 mentions) C57bl 6 mice, by Charles River Laboratories (1 mentions) 7 0 nylon suture, by Johnson & Johnson (1 mentions) Marcain, by AstraZeneca (1 mentions) 5 0 absorbable suture, by Johnson & Johnson (1 mentions) C57bl 6j mice, by Charles River Laboratories (1 mentions) 7 0 silk suture, by Johnson & Johnson (1 mentions) Xylazine, by Bayer (1 mentions) Ketamine, by Pfizer (1 mentions) Ventilator, by Harvard Apparatus (1 mentions) 0.3 ml insulin syringe, by BD (1 mentions) Escherichia coli o55 b5, by Merck Group (1 mentions) Filtration system, by Merck Group (1 mentions) Citric acid, by Merck Group (1 mentions) Sodium hydroxide (naoh), by Merck Group (1 mentions) Sodium phosphate, by Merck Group (1 mentions)

30 protocols using 6 0 silk suture

1

Ischemic Myocardium Stem Cell Therapy

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Twenty-four hours before surgery, the animals were pre-medicated with a fentanyl patch, and were administered 12 mg/kg ketamine (im), midazolam 0.5 mg/kg (im), and tramadol 5 mg/kg (im) 15 min before the intervention. The animals were anesthetized using isoflurane 2% and a continuous infusion of morphine (12 mg), ketamine (30 mg) and lidocaine (15 mg) in 500 ml saline solution at a rate of 10 ml/kg/h throughout the intervention.
A lateral thoracotomy was performed, and the anterior descending artery was ligated by 6/0 silk suture (Ethicon). One hour after ligation, 5 × 106 stem cells or saline solution (1 ml) were delivered by syringe into the myocardial ischemic tissue around the infarct area (at 3 points). After surgery, the animals were kept individually isolated in a 2 m2 space with controlled food and water ad libitum. Analgesia was provided with a fentanyl patch every 2 days for a week, and the animals tolerated food the day after surgery. Ceftriaxone 40 mg/kg (im) was used as antibiotic prophylaxis from 3 days before until 72 h after the intervention.
García Gómez-Heras S., Largo C., Larrea J.L., Vega-Clemente L., Calderón Flores M., Ruiz-Pérez D., García-Olmo D, & García-Arranz M. (2019). Main histological parameters to be evaluated in an experimental model of myocardial infarct treated by stem cells on pigs. PeerJ, 7, e7160.
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2

Spared Nerve Injury Model of Neuropathic Pain

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The spared nerve injury (SNI) model of neuropathic pain was performed under Avertine (2,2,2-tribromoethanol, Sigma-Aldrich) general anesthesia, as previously described (7 (link), 41 (link)). Briefly, skin and muscle incisions were made on the left hind leg at mid-thigh level, revealing the sciatic nerve and its three branches. The common peroneal and sural nerves were carefully ligated with 6.0 silk suture (Ethicon, Johnson & Johnson Intl.), transected, and a 1–2mm sections of each of these nerves were removed. The tibial nerve was left intact. Skin was then closed and stitched with silk 4.0 sutures (Ethicon, Johnson & Johnson Intl). Sham operated mice were exposed to the same procedure but all nerves were left intact.
Descalzi G., Mitsi V., Purushothaman I., Gaspari S., Avrampou K., Loh Y.H., Shen L, & Zachariou V. (2017). Neuropathic pain promotes gene expression adaptations in brain networks involved in stress and depression. Science signaling, 10(471), eaaj1549.
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3

Spared Nerve Injury Surgical Protocol

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Spared nerve injury (SNI) surgeries were performed as previously described [14 (link)–16 (link)]. Briefly, rats were induced at 5% isoflurane (Abbott Laboratories, Chicago, IL, USA) and maintained at 2.5% during surgical procedures. An incision was made in the skin at the level of the trifurcation of the left or right sciatic nerve. The overlaying muscles were retracted, exposing the peroneal, tibial and sural nerves. The common peroneal and tibial nerves were ligated with 6.0 silk suture (Ethicon, Somerville, NJ, USA) and the knot and adjacent nerve (2.0 mm) were transected. Care was taken to avoid disturbing the sural branch. Following SNI, the muscle was sutured with absorbable 4.0 sutures (Ethicon) and the skin closed with wound clips.
Cooper A.H., Brightwell J.J., Hedden N.S, & Taylor B.K. (2018). The left central nucleus of the amygdala contributes to mechanical allodynia and hyperalgesia following right-sided peripheral nerve injury. Neuroscience letters, 684, 187-192.
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4

Mouse Model of Myocardial Infarction

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MI was modeled by ligating the left anterior descending artery. Mice were anesthetized with isoflurane (3% isoflurane for induction and 2% isoflurane for maintenance) and intubated with the 20‐G catheter into the trachea. The catheter was connected to a ventilator (for a mouse with the body weight of 25 g, the tidal volume was set at 225 μL and respiratory rate at 130 times per minute). Also, 100% oxygen was flowed into the ventilator. The thoracic cavity was cut through the left parasternal incision, and the heart was exposed in the 3rd to 4th intercostal space. The pericardium was opened and the left anterior descending artery was ligated with an 8‐0 silk suture (Ethicon). The dissected intercostal space and chest skin were closed with a 6‐0 silk suture (Ethicon). Sham‐operated mice were treated without left anterior descending ligation.24
Wan J., Lin S., Yu Z., Song Z., Lin X., Xu R, & Du S. (2022). Protective Effects of MicroRNA‐200b‐3p Encapsulated by Mesenchymal Stem Cells–Secreted Extracellular Vesicles in Myocardial Infarction Via Regulating BCL2L11. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 11(12), e024330.
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5

Murine Myocardial Infarction Model

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MI was performed on 8-week-old male c57BL/6 mice (Jackson Laboratory) by ligation of the LAD coronary artery. For surgery, mice are anesthetized with isoflurane (3% isoflurane for induction, 2% isoflurane for maintenance). The chest is shaved and cleaned with alcohol. A suture is placed around the front upper incisors and pulled taut so that the neck is slightly extended. The tongue is retracted and held with forceps, and a 20-G catheter is inserted into the trachea. The catheter is then attached to the mouse ventilator via a Y-shaped connector. Ventilation is performed with a tidal volume of 225 μl for a 25 g mouse and a respiratory rate of 130 breaths per minute. 100% oxygen is provided to the inflow of the ventilator. The chest is opened through a left parasternal incision, and the heart exposed at the left 3rd–4th intercostal space. Chest retractor is applied to facilitate the view. The pericardium is opened, and ligations made on the LAD coronary artery using 8–0 silk sutures (Ethicon). The lungs are slightly overinflated to assist in removal of air in the pleural cavity. Dissected intercostal space and chest skin were closed using a 6–0 silk suture (Ethicon).
Gao F., Kataoka M., Liu N., Liang T., Huang Z.P., Gu F., Ding J., Liu J., Zhang F., Ma Q., Wang Y., Zhang M., Hu X., Kyselovic J., Hu X., Pu W.T., Wang J., Chen J, & Wang D.Z. (2019). Therapeutic role of miR-19a/19b in cardiac regeneration and protection from myocardial infarction. Nature Communications, 10, 1802.
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6

Hind Limb Ischemia Induction in Mice

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To induce unilateral hind limb ischemia (HLI), the ligation of the left femoral artery (FA) was performed according to the procedure described by Niiyama et al. established at the Stanford University, CA, USA [21 ]. In the male C57BL/6 mice (Charles River Laboratories, L'Arbresle, France) at the aged of 6 to 8 weeks, the distal and proximal ends of the FA were occluded with 6–0 silk suture (Ethicon). The segment of FA between the distal and proximal knots were carefully transected. The wound was then closed and dressed. Mice were randomly divided into two subgroups (N = 8–9/group): (1) vehicle (PBS, control group) and (2) MSC-EVs (EV-treated group). Respectively, PBS or MSC-EVs (10 µg/mL of blood), respectively, were administered intravenously through the tail vein every 3 days for 21 days of the experiment.
The total blood volume for each mouse was calculated based on the consideration that the total blood volume in the mouse is in the range of 6–8 mL/100 g of body weight [22 ] as shown in the Additional file 1: Table S1. At 7, 14 and 21 days after FA occlusion, blood flow was measured in both limbs as described below. At 21 days after HLI, animals were sacrificed and muscle tissues were harvested for further biochemical and histological analysis. The in vivo experimental design is presented in Additional file 1: Fig. S1.
Łabędź-Masłowska A., Vergori L., Kędracka-Krok S., Karnas E., Bobis-Wozowicz S., Sekuła-Stryjewska M., Sarna M., Andriantsitohaina R, & Zuba-Surma E.K. (2024). Mesenchymal stem cell-derived extracellular vesicles exert pro-angiogenic and pro-lymphangiogenic effects in ischemic tissues by transferring various microRNAs and proteins including ITGa5 and NRP1. Journal of Nanobiotechnology, 22, 60.
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7

Aortic Ligation in Anesthetized Mice

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Mice were anesthetized with isoflurane (3–4% isoflurane for induction, 1–2% isoflurane for maintenance). The chest was shaved and cleaned with alcohol. A suture was placed around the front upper incisors and pulled taut so that the neck was slightly extended. The tongue was retracted and held with forceps, and a 20-G catheter was inserted into the trachea. The catheter was then attached to the mouse ventilator via a Y-shaped connector. Ventilation was performed with a tidal volume of 220–240 μl for a 25–30 g mouse and a respiratory rate of 130–140 breaths per min. 100% oxygen was provided to the inflow of the ventilator. The chest was opened through a left 2nd intercostal thoracotomy. The 26-G needle without its sharp tip was put on the ascending aorta. They were tightly ligated together using 7-0 Nylon suture (Ethicon, Edinburgh, Scotland) at the position between brachiocephalic artery and left common carotid artery, and the 26-G needle was removed immediately after ligation. In the sham operation, no ligation was performed. Isoflurane was stopped, and the lungs were slightly overinflated to assist in removal of air in the pleural cavity. Dissected intercostal space and chest skin were closed using 6-0 silk suture (Ethicon, Edinburgh, Scotland). All manipulations were performed by an operator without knowledge of genotype.
Yan Y., Long T., Su Q., Wang Y., Chen K., Yang T., Zhao G., Ma Q., Hu X., Liu C., Liao X., Min W., Li S., Zhang D., Yang Y., Pu W.T., Dong Y., Wang D.Z., Chen Y, & Huang Z.P. (2022). Cardiac ISL1-Interacting Protein, a Cardioprotective Factor, Inhibits the Transition From Cardiac Hypertrophy to Heart Failure. Frontiers in Cardiovascular Medicine, 9, 857049.
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8

Spared Nerve Injury Model of Neuropathic Pain

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The spared nerve injury (SNI) model of neuropathic pain was performed under Avertine (2,2,2-tribromoethanol, Aldrich) general anesthesia, as previously described (Shields et al 2003 (link), Stratinaki et al 2013 ). Skin and muscle incision of the left hind-limp at mid-thigh level revealed the sciatic nerve and its three branches, with the help of a stereomicroscope. The common peroneal and the sural nerves were carefully ligated with 6.0 silk suture (Ethicon, Johnson & Johnson Intl.), transected and 1–2mm sections of these nerves were removed, while the tibial nerve was left intact. Skin was then closed with silk 4.0 sutures (Ethicon, Johnson & Johnson Intl). In sham-operated mice the same procedure was followed but the nerves were left intact.
Terzi D., Gaspari S., Manouras L., Descalzi G., Mitsi V, & Zachariou V. (2014). RGS9-2 modulates sensory and mood related symptoms of neuropathic pain. Neurobiology of learning and memory, 115, 43-48.
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9

Establishing Rat Myocardial Infarction Model

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To establish an IR injury model, all adult male Sprague-Dawley rats (230±10 g) were anesthetized by intramuscular injection of ketamine hydrochloride (80 mg/kg) and xylazine hydrochloride (4 mg/kg). The rats were intubated, and were ventilated with positive pressure (180 mL/min) using a ventilator (Harvard Apparatus model 683, Millis, MA, USA). The small incision was operated at the left lateral costal rib to expose the heart. The left anterior descending artery was ligated for 1 h with a 6-0 silk suture (Ethicon, Somerville, NJ, USA), followed by reperfusion for 3 h. Immediately after the initiation of reperfusion, 125 µL of an EV solution (0.4 µg/µL) in phosphate buffered saline (PBS) (IR+EVNM or IR+EVHM group) or PBS alone (IR group) was systemically injected via the leg veins of the rats. We used sham-operated rats as controls. The animals were sacrificed 3 hours after the EV injection.
To measure the myocardial infarcted size, hearts were sectioned and incubated in 1% 2,3,5-triphenyltetrazolium chloride (TTC; Sigma-Aldrich, St Louis, MO, USA) for 30 min at 37℃. Each heart specimen was fixed for 24 h in 10% paraformaldehyde. Myocardium was identified as red, whereas infarcted areas appeared yellow. The region of normal and infarcted left ventricular myocardium was directly assessed by planimetry using dedicated software (ImageJ software, NIH software).
Park H., Park H., Mun D., Kang J., Kim H., Kim M., Cui S., Lee S.H, & Joung B. (2018). Extracellular Vesicles Derived from Hypoxic Human Mesenchymal Stem Cells Attenuate GSK3β Expression via miRNA-26a in an Ischemia-Reperfusion Injury Model. Yonsei Medical Journal, 59(6), 736-745.
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10

Neonatal Hypoxic-Ischemic Injury Model

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Unilateral HI was induced in CX3CR1GFP/+CCR2RFP/+ pups of both sexes at P9 according to the Vannucci model, with some modification.10 (link),95 (link),96 (link) Animals were anesthetized with 5% isoflurane for induction and 1.5% isoflurane for maintenance in a mixture of air and oxygen (1:1), and the right common carotid artery was ligated with a 6-0 silk suture (Ethicon Inc.). After the surgical procedure, the wounds were infiltrated with bupivacaine (2.5 mg/mL, Marcain; AstraZeneca) for anesthesia. The animals were returned to the dam for 2 h and placed in a chamber perfused with a humidified gas mixture (10% oxygen in nitrogen) for 50 min at 36°C. Their axillary temperature was measured by the thermal probe in the skin pocket between upper foreleg and chest of the animal for about 30 seconds. Control male and female pups were neither subjected to ligation nor hypoxia.
Di Martino E., Ambikan A., Ramsköld D., Umekawa T., Giatrellis S., Vacondio D., Romero A.L., Galán M.G., Sandberg R., Ådén U., Lauschke V.M., Neogi U., Blomgren K, & Kele J. (2024). Inflammatory, metabolic, and sex-dependent gene-regulatory dynamics of microglia and macrophages in neonatal hippocampus after hypoxia-ischemia. iScience, 27(4), 109346.
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