Bd555742

Manufactured by BD

The BD555742 is a laboratory equipment item. It is designed to perform a core function within a laboratory setting, but a detailed description cannot be provided while maintaining an unbiased and factual approach. More information from the manufacturer would be required to describe the product's specific capabilities and applications.

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Lab products found in correlation

Facscalibur, by BD (8 mentions) Bd555478, by BD (4 mentions) Macs system, by Miltenyi Biotec (2 mentions) Pe conjugated cd133, by Miltenyi Biotec (2 mentions) Cellquest software, by BD (2 mentions) Magnetic cell sorting system, by Miltenyi Biotec (2 mentions) Magnetic activated cell sorting system, by Miltenyi Biotec (1 mentions) Fitc conjugated cd44 antibody, by BD (1 mentions) Fitc conjugated cd44, by BD (1 mentions) Accutase, by Innovative Cell Technologies (1 mentions) Facscalibur platform, by BD (1 mentions)

9 protocols using bd555742

CD44+ Cell Isolation and Quantification

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For FACS, cells were dissociated using Accutase and resuspended in PBS containing 0.5% BSA. The cells were stained with FITC-conjugated CD44 (BD555478) or isotype control antibody (BD555742) from BD Biosciences on ice for 30 min. Cells were then washed with PBS and analyzed on a BD FACSCalibur (BD Biosciences) using Cell Quest software.
CD44-positive cells were sorted by a MACS system (Miltenyi Biotech). Cells were dissociated using Accutase, resuspended in PBS, and stained with CD44-Micro Beads on ice for 30 min. Cells were then passed through a LS magnetic column where CD44-positive cells were retained. The CD44-positive cells were then eluted from the column after removal from the magnet. Quantitative analysis of CD44-positive cells was performed by immunofluorescence using FITC-conjugated CD44 antibody (555478, BD Biosciences).

Yoon C., Cho S.J., Aksoy B.A., Park D.J., Schultz N., Ryeom S.W, & Yoon S.S. (2015). Chemotherapy resistance in diffuse type gastric adenocarcinoma is mediated by RhoA activation in cancer stem-like cells. Clinical cancer research : an official journal of the American Association for Cancer Research, 22(4), 971-983.

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CD44-Positive Cell Isolation and Quantification

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For FACS, cells were dissociated using Accutase and resuspended in PBS containing 0.5% BSA. The cells were stained with FITC-conjugated CD44 (BD555478) or isotype control antibody (BD555742) from BD Biosciences on ice for 30 min. Cells were then washed with PBS and analyzed on a BD FACSCalibur (BD Biosciences, San Jose, CA) using Cell Quest software.
CD44-positive cells were sorted by a magnetic-activated cell sorting (MACS) system (Miltenyi Biotech, San Diego, CA). After collecting spheroids, cells were washed with phosphate-buffered saline (PBS), dissociated to single cells using Accutase, and stained with CD44-Micro Beads on ice for 30 min. Cells were then passed through a LS magnetic column where CD44-positive cells were retained. The CD44-positive cells were then eluted from the column after removal from the magnet. Quantitative analysis of CD44-positive cells was performed by immunofluorescence using FITC-conjugated CD44 antibody.

Yoon C., Park D.J., Schmidt B., Thomas N.J., Lee H.J., Kim T.S., Janjigian Y.Y., Cohen D.J, & Yoon S.S. (2014). CD44 expression denotes a subpopulation of gastric cancer cells in which Hedgehog signaling promotes chemotherapy resistance. Clinical cancer research : an official journal of the American Association for Cancer Research, 20(15), 3974-3988.

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CD44 Expression Analysis by Flow Cytometry

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Cells were dissociated using Accutase and resuspended in PBS containing 0.5% BSA. The cells were stained with FITC-conjugated CD44 (BD555478) or isotype control antibody (BD555742) from BD Biosciences on ice for 30 min. Cells were then washed with PBS and analyzed on a BD FACSCalibur (BD Biosciences) using Cell Quest software.

Cho S.J., Yoon C., Lee J.H., Chang K.K., Lin J.X., Kim Y.H., Kook M.C., Aksoy B.A., Park D.J., Ashktorab H., Smoot D.T., Schultz N, & Yoon S.S. (2018). KMT2C mutations in diffuse type gastric adenocarcinoma promote epithelial-to-mesenchymal transition. Clinical cancer research : an official journal of the American Association for Cancer Research, 24(24), 6556-6569.

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CD133 Expression Analysis by Flow Cytometry

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Cells were dissociated using Accutase and resuspended in phosphate-buffered saline (PBS) containing 0.5% bovine serum albumin (BSA). The cells were stained with PE-conjugated CD133 (130-098-826; Miltenyi Biotec) or isotype control antibody (BD555742; BD Biosciences) on ice for 30 min. Cells were then washed with PBS and analyzed on a BD FACSCalibur™ (BD Biosciences) using BD CellQuest software (BD Biosciences).

Chang K.K., Yoon C., Yi B.C., Tap W.D., Simon M.C, & Yoon S.S. (2018). Platelet-derived growth factor receptor-α and -β promote cancer stem cell phenotypes in sarcomas. Oncogenesis, 7(6), 47.

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Isolation and Quantification of CD133+ Cells

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For FACS, cells were dissociated using Accutase and resuspended in PBS containing 0.5% BSA. The cells were stained with PE-conjugated CD133 (BD566593) or isotype control antibody (BD555742) from BD Biosciences on ice for 30 min. Cells were then washed with PBS and analyzed on a BD FACSCalibur (BD Biosciences) using Cell Quest software.
CD133+ cells were sorted by a magnetic-activated cell sorting system (Miltenyi Biotech). After collecting spheroids, cells were washed with PBS, dissociated to single cells using Accutase, and stained with CD133-Micro Beads on ice for 30 min. Cells were then passed through an LS magnetic column, where CD133+ cells were retained. The CD133+ cells were then eluted from the column after removal from the magnet. Quantitative analysis of CD133+ cells was performed by immunofluorescence using PE-conjugated CD133 antibody.

Yoon C., Lu J., Yi B.C., Chang K.K., Simon M.C., Ryeom S, & Yoon S.S. (2021). PI3K/Akt pathway and Nanog maintain cancer stem cells in sarcomas. Oncogenesis, 10(1), 12.

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CD133 Expression Analysis by Flow Cytometry

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Cells were dissociated using Accutase and resuspended in phosphate-buffered saline (PBS) containing 0.5% bovine serum albumin (BSA). The cells were stained with PE-conjugated CD133 (130-098-826; Miltenyi Biotec) or isotype control antibody (BD555742; BD Biosciences) on ice for 30 min. Cells were then washed with PBS and analyzed on a BD FACSCalibur^™ (BD Biosciences) using BD CellQuest software (BD Biosciences).

Yoon C., Lu J., Ryeom S.W., Simon M.C, & Yoon S.S. (2021). PIK3R3, part of the regulatory domain of PI3K, is upregulated in sarcoma stem-like cells and promotes invasion, migration, and chemotherapy resistance. Cell Death & Disease, 12(8), 749.

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Cell Sorting and Quantification via CD44

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For cell sorting, cells were dissociated using Accutase (Innovative Cell Technologies). For FACS, cells were resuspended in PBS containing 0.5% BSA, stained with FITC-conjugated CD44 (BD555478) or isotype control antibody (BD555742) from BD Biosciences and analysed on a BD FACSCalibur (BD Biosciences) using Cell Quest software. CD44-positive cells were sorted using a magnetic cell sorting system (Miltenyi Biotech). Cells were stained with CD44 Micro-Beads, passed through a LS magnetic column, then eluted from the column after removal from the magnet. CD44-positive cells were quantified by immunofluorescence using FITC-conjugated CD44 antibody (555478; BD Biosciences).

Lin J.X., Yoon C., Li P., Ryeom S.W., Cho S.J., Zheng C.H., Xie J.W., Wang J.B., Lu J., Chen Q.Y., Yoon S.S, & Huang C.M. (2020). CDK5RAP3 as tumour suppressor negatively regulates self-renewal and invasion and is regulated by ERK1/2 signalling in human gastric cancer. British Journal of Cancer, 123(7), 1131-1144.

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Isolation and Analysis of CD44-Positive Cells

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For fluorescence-activated cell sorting (FACS), cells were dissociated using Accutase and resuspended in phosphate-buffered saline (PBS) containing 0.5% bovine serum albumin (BSA). The cells were stained with fluorescein isothiocyanate (FITC)-conjugated anti-CD44 (BD555478; BD Biosciences) or isotype control antibody (BD555742; BD Biosciences) and then analyzed on a FACSCalibur platform (BD Biosciences) using Cell Quest software. CD44-positive cells were collected using a magnetic cell sorting system (MiltenyiBiotec, BergischGaldbach, Germany). In brief, cells were dissociated using Accutase, stained with CD44-Micro Beads, and passed through a LS magnetic column that retains CD44-positive cells. CD44-positive cells were then eluted from the column after removal of the magnet and quantified by immunofluorescence (IF) using FITC-conjugated CD44 antibodies.

Huang C., Yoon C., Zhou X.H., Zhou Y.C., Zhou W.W., Liu H., Yang X., Lu J., Lee S.Y, & Huang K. (2020). ERK1/2-Nanog signaling pathway enhances CD44(+) cancer stem-like cell phenotypes and epithelial-to-mesenchymal transition in head and neck squamous cell carcinomas. Cell Death & Disease, 11(4), 266.

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CD44 Expression Analysis by Flow Cytometry

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Yoon C., Cho S.J., Chang K.K., Park D.J., Ryeom S.W, & Yoon S.S. (2017). Role of Rac1 pathway in epithelial-to-mesenchymal transition and cancer stem-like cell phenotypes in gastric adenocarcinoma. Molecular cancer research : MCR, 15(8), 1106-1116.

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