CD44-positive cells were sorted by a MACS system (Miltenyi Biotech). Cells were dissociated using Accutase, resuspended in PBS, and stained with CD44-Micro Beads on ice for 30 min. Cells were then passed through a LS magnetic column where CD44-positive cells were retained. The CD44-positive cells were then eluted from the column after removal from the magnet. Quantitative analysis of CD44-positive cells was performed by immunofluorescence using FITC-conjugated CD44 antibody (555478, BD Biosciences).
Bd555742
The BD555742 is a laboratory equipment item. It is designed to perform a core function within a laboratory setting, but a detailed description cannot be provided while maintaining an unbiased and factual approach. More information from the manufacturer would be required to describe the product's specific capabilities and applications.
Lab products found in correlation
9 protocols using bd555742
CD44+ Cell Isolation and Quantification
CD44-Positive Cell Isolation and Quantification
CD44 Expression Analysis by Flow Cytometry
CD133 Expression Analysis by Flow Cytometry
Isolation and Quantification of CD133+ Cells
CD133+ cells were sorted by a magnetic-activated cell sorting system (Miltenyi Biotech). After collecting spheroids, cells were washed with PBS, dissociated to single cells using Accutase, and stained with CD133-Micro Beads on ice for 30 min. Cells were then passed through an LS magnetic column, where CD133+ cells were retained. The CD133+ cells were then eluted from the column after removal from the magnet. Quantitative analysis of CD133+ cells was performed by immunofluorescence using PE-conjugated CD133 antibody.
CD133 Expression Analysis by Flow Cytometry
Cell Sorting and Quantification via CD44
Isolation and Analysis of CD44-Positive Cells
CD44 Expression Analysis by Flow Cytometry
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!