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Bd555742

Manufactured by BD

The BD555742 is a laboratory equipment item. It is designed to perform a core function within a laboratory setting, but a detailed description cannot be provided while maintaining an unbiased and factual approach. More information from the manufacturer would be required to describe the product's specific capabilities and applications.

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9 protocols using bd555742

1

CD44+ Cell Isolation and Quantification

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For FACS, cells were dissociated using Accutase and resuspended in PBS containing 0.5% BSA. The cells were stained with FITC-conjugated CD44 (BD555478) or isotype control antibody (BD555742) from BD Biosciences on ice for 30 min. Cells were then washed with PBS and analyzed on a BD FACSCalibur (BD Biosciences) using Cell Quest software.
CD44-positive cells were sorted by a MACS system (Miltenyi Biotech). Cells were dissociated using Accutase, resuspended in PBS, and stained with CD44-Micro Beads on ice for 30 min. Cells were then passed through a LS magnetic column where CD44-positive cells were retained. The CD44-positive cells were then eluted from the column after removal from the magnet. Quantitative analysis of CD44-positive cells was performed by immunofluorescence using FITC-conjugated CD44 antibody (555478, BD Biosciences).
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2

CD44-Positive Cell Isolation and Quantification

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For FACS, cells were dissociated using Accutase and resuspended in PBS containing 0.5% BSA. The cells were stained with FITC-conjugated CD44 (BD555478) or isotype control antibody (BD555742) from BD Biosciences on ice for 30 min. Cells were then washed with PBS and analyzed on a BD FACSCalibur (BD Biosciences, San Jose, CA) using Cell Quest software.
CD44-positive cells were sorted by a magnetic-activated cell sorting (MACS) system (Miltenyi Biotech, San Diego, CA). After collecting spheroids, cells were washed with phosphate-buffered saline (PBS), dissociated to single cells using Accutase, and stained with CD44-Micro Beads on ice for 30 min. Cells were then passed through a LS magnetic column where CD44-positive cells were retained. The CD44-positive cells were then eluted from the column after removal from the magnet. Quantitative analysis of CD44-positive cells was performed by immunofluorescence using FITC-conjugated CD44 antibody.
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3

CD44 Expression Analysis by Flow Cytometry

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Cells were dissociated using Accutase and resuspended in PBS containing 0.5% BSA. The cells were stained with FITC-conjugated CD44 (BD555478) or isotype control antibody (BD555742) from BD Biosciences on ice for 30 min. Cells were then washed with PBS and analyzed on a BD FACSCalibur (BD Biosciences) using Cell Quest software.
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4

CD133 Expression Analysis by Flow Cytometry

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Cells were dissociated using Accutase and resuspended in phosphate-buffered saline (PBS) containing 0.5% bovine serum albumin (BSA). The cells were stained with PE-conjugated CD133 (130-098-826; Miltenyi Biotec) or isotype control antibody (BD555742; BD Biosciences) on ice for 30 min. Cells were then washed with PBS and analyzed on a BD FACSCalibur™ (BD Biosciences) using BD CellQuest software (BD Biosciences).
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5

Isolation and Quantification of CD133+ Cells

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For FACS, cells were dissociated using Accutase and resuspended in PBS containing 0.5% BSA. The cells were stained with PE-conjugated CD133 (BD566593) or isotype control antibody (BD555742) from BD Biosciences on ice for 30 min. Cells were then washed with PBS and analyzed on a BD FACSCalibur (BD Biosciences) using Cell Quest software.
CD133+ cells were sorted by a magnetic-activated cell sorting system (Miltenyi Biotech). After collecting spheroids, cells were washed with PBS, dissociated to single cells using Accutase, and stained with CD133-Micro Beads on ice for 30 min. Cells were then passed through an LS magnetic column, where CD133+ cells were retained. The CD133+ cells were then eluted from the column after removal from the magnet. Quantitative analysis of CD133+ cells was performed by immunofluorescence using PE-conjugated CD133 antibody.
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6

CD133 Expression Analysis by Flow Cytometry

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Cells were dissociated using Accutase and resuspended in phosphate-buffered saline (PBS) containing 0.5% bovine serum albumin (BSA). The cells were stained with PE-conjugated CD133 (130-098-826; Miltenyi Biotec) or isotype control antibody (BD555742; BD Biosciences) on ice for 30 min. Cells were then washed with PBS and analyzed on a BD FACSCalibur (BD Biosciences) using BD CellQuest software (BD Biosciences).
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7

Cell Sorting and Quantification via CD44

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For cell sorting, cells were dissociated using Accutase (Innovative Cell Technologies). For FACS, cells were resuspended in PBS containing 0.5% BSA, stained with FITC-conjugated CD44 (BD555478) or isotype control antibody (BD555742) from BD Biosciences and analysed on a BD FACSCalibur (BD Biosciences) using Cell Quest software. CD44-positive cells were sorted using a magnetic cell sorting system (Miltenyi Biotech). Cells were stained with CD44 Micro-Beads, passed through a LS magnetic column, then eluted from the column after removal from the magnet. CD44-positive cells were quantified by immunofluorescence using FITC-conjugated CD44 antibody (555478; BD Biosciences).
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8

Isolation and Analysis of CD44-Positive Cells

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For fluorescence-activated cell sorting (FACS), cells were dissociated using Accutase and resuspended in phosphate-buffered saline (PBS) containing 0.5% bovine serum albumin (BSA). The cells were stained with fluorescein isothiocyanate (FITC)-conjugated anti-CD44 (BD555478; BD Biosciences) or isotype control antibody (BD555742; BD Biosciences) and then analyzed on a FACSCalibur platform (BD Biosciences) using Cell Quest software. CD44-positive cells were collected using a magnetic cell sorting system (MiltenyiBiotec, BergischGaldbach, Germany). In brief, cells were dissociated using Accutase, stained with CD44-Micro Beads, and passed through a LS magnetic column that retains CD44-positive cells. CD44-positive cells were then eluted from the column after removal of the magnet and quantified by immunofluorescence (IF) using FITC-conjugated CD44 antibodies.
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9

CD44 Expression Analysis by Flow Cytometry

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Cells were dissociated using Accutase and resuspended in PBS containing 0.5% BSA. The cells were stained with FITC-conjugated CD44 (BD555478) or isotype control antibody (BD555742) from BD Biosciences on ice for 30 min. Cells were then washed with PBS and analyzed on a BD FACSCalibur (BD Biosciences) using Cell Quest software.
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