Immunofluorescence was performed as described previously [45 (link)]. Cells were seeded into the 6-well culture plate (Corning Costar Corp) to prepare for performing cell immunofluorescence (IF). After incubating with primary antibodies, cells then incubated with corresponding fluorescence labeled secondary antibody. The slides were photographed using the inverted fluorescence microscope TE-2000S (Nikon, Tokyo, Japan). Primary antibodies for E-cadherin, vimentin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Rhodamine-conjugated phalloidin, DAPI and fluorescence labeled secondary antibody were obtained from Beyotime Institute of Biotechnology (Shanghai, China).
Fluorescence labeled secondary antibody
Manufactured by Beyotime
Sourced in China
Fluorescence labeled secondary antibody is a laboratory reagent used to detect and visualize target proteins in various biological samples. It consists of a secondary antibody conjugated with a fluorescent dye, which binds to the primary antibody specific to the target protein. This allows for the indirect detection and localization of the target protein through fluorescence microscopy or other fluorescence-based techniques.
Automatically generated - may contain errors
Lab products found in correlation
6 well culture plate, by Corning (2 mentions)
4 6 diamidino 2 phenylindole (dapi), by Beyotime (2 mentions)
Te2000 s, by Nikon (2 mentions)
E cadherin, by Santa Cruz Biotechnology (2 mentions)
Rhodamine conjugated phalloidin, by Beyotime (2 mentions)
Vimentin, by Santa Cruz Biotechnology (1 mentions)
Rabbit anti mouse nf κb p65 antibody, by Cell Signaling Technology (1 mentions)
3 protocols using fluorescence labeled secondary antibody
1
Immunofluorescence Imaging of Cell Markers
2
Cell Immunofluorescence Microscopy Protocol
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Stably transfected cells were seeded in the 6-well Culture plate (Corning Costar Corp, Corning, NY) to prepare for performed cell immunofluorescence (IF) and incubated with primary antibodies then incubated with fluorescence labeled secondary antibody. The slides were imaged using a microscope or inverted fluorescence microscope TE-2000S (Nikon, Tokyo, Japan). Primary antibodies for E-cadherin, vimentin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Rhodamine-conjugated phalloidin, DAPI and fluorescence labeled secondary antibody were obtained from Beyotime Institute of Biotechnology (Shanghai, China).
3
Immunofluorescence Staining of Cells
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For immunofluorescence staining, cells were fixed in 4% paraformaldehyde and penetrated with 0.2% Triton X-100 and 0.04% SDS in PBS. The cells were incubated with 1 : 500 diluted rabbit anti-mouse beta-tubulin III antibody (Santa Cruz, CA, USA) for neuron identification or 1 : 1000 diluted rabbit anti-mouse NF-κB p65 antibody (Cell Signaling, Danvers, MA, USA) for NF-κB location. Later, the cells were incubated with fluorescence-labeled secondary antibody (Beyotime, Haimen, China) and 1 μg/mL Hoechst 33258. The processed cells were imaged by fluorescence microscopy.
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