Mouse il 1β elisa kit

Blood samples collected from the experimental animals were centrifuged at 1000× g for 15 min to obtain serum. IL-1β, LPS, TNF-α in serum were measured by mouse IL-1β ELISA Kit (Neobioscience, Shenzhen, China), mouse LPS ELISA Kit (CUSABIO, Wuhan, China), and mouse TNF-α ELISA Kit (Neobioscience, Shenzhen, China), respectively.
bEnd.3 cells were treated with HSYA (10 μM) or NAC (2 mM) in the presence of LPS (100 ng/mL) for 16 h, referring to the dose in the published literature [11 (link),13 (link)]. The cell supernatant was collected and lactate concentration was measured according to the manufacturer’s instructions (Jiancheng, A019-2-1, Nanjing, China). Additionally, the treated bEnd.3 cells were washed with PBS and dissociated with trypsin, then the freeze–thaw process was repeated to lyse cells. After centrifuging at 1500× g for 10 min at 4 °C, the supernatant was collected for the measurement of 4-HNE according to the manufacturer’s protocol of the 4-HNE ELISA Kit (Elabscience, Wuhan, China).