Rabbit anti cxcr4
Rabbit anti-CXCR4 is a primary antibody that recognizes the CXCR4 protein, a chemokine receptor expressed on the surface of various cell types. This antibody can be used for the detection and study of CXCR4 in research applications.
Lab products found in correlation
13 protocols using rabbit anti cxcr4
Western Blot and Immunofluorescence Antibody Conditions
Quantifying Angiogenesis in Ischemic Limbs
Measuring CXCR4 in Rat Hypothalamus
Western Blot Analysis of CXCL12/CXCR4 Signaling
Western Blot Analysis of CXCR4 and TNF-α
Quantifying CXCL12 and CXCR4 Protein Levels
Immunoreactive bands were visualized using LiteAblot Turbo luminol reagents (EuroClone) and Hyperfilm- ECL film (EuroClone) according to the manufacturer’s instructions. To normalize the bands, filters were stripped and re-probed with a monoclonal anti-α-tubulin (Sigma-Aldrich). Band density was quantified densitometrically.
HA-Lm Gel Modulates CXCR4 Expression
Immunohistochemical Analysis of Brain Sections
Immunostaining of Vascular Cell Markers
For quantification, five immunostained sections from each animal were used. Positively stained cells were counted (based on fluorescence intensity) using ImageJ by a student blinded to experimental conditions. The numbers from all five sections were pooled to generate the mean for each animal. The means from all the animals in each treatment group were then averaged, and the SEM was calculated.
Colocalization of CXCR4 and CD74 in Endothelial Cells
a concentration 4 × 104 cells per well for 24 h at
37 °C. Next, the cells were infected with P.
gingivalis at an MOI of 100 for 24 h. The cells were subsequently
washed and fixed, after which they were incubated in 1% BSA in 0.1% PBS-Tween
for 1 h. Afterwards, the samples were incubated with mouse anti-CD74 (1:100;
Santa Cruz) and rabbit anti-CXCR4 (1:200; Abcam) overnight at 4 °C. Donkey
anti-mouse IgG labeled with PE (1:20; Proteintech) and goat anti-rabbit IgG
labeled with FITC (1:20; Proteintech) were used as the secondary antibodies and
were incubated with the cells for 1 h at room temperature. DAPI (Boster, Wuhan,
China) was used to stain the cell nucleus (pseudo-colored blue), and the cells
were observed using a fluorescence microscope (Nikon 80i, Japan) to evaluate the
colocalization between CXCR4 and CD74.
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