3 aminopropyltriethoxysilane aptes

Photolithography and e-beam evaporation was used to form the microelectrodes by depositing a 20-nm Ti adhesion layer and a 180-nm Au gold contact layer onto the 300-nm SiO₂ layer of a silicon wafer [33 (link)]. Each microelectrode has single sensor consisting of 10 pairs of 5 μm wide interdigitated fingers separated by a 3-μm gap. A chip has five independent microelectrodes with the sensors located in close proximity to one another, forming a sensing region. Each chip was bathed in 30% ammonium hydroxide (Fisher Scientific, Inc., Hampton, NH, USA, A669-212) for 30 min and rinsed with deionized water (DI). Next, the sensing region was covered by 3-aminopropyltriethoxysilane (APTES) (Acros Organics, Fair Lawn, NJ, USA, 99%) for 30 min followed by washing in DI. A 10 μL drop of 0.01 mg/mL of a 95% semiconductive SWNT suspension (Nanointegris Inc., Boisbriand, QC, Canada) was dropcasted onto the sensing region for 1 h followed by annealing for 1 h at 250 °C in an open-ended quartz tube resulting in a self-assembled network of SWNT. Annealed chips were stored under vacuum until use.

ASA LI 941 NC was purchased from LG Chem Ltd. (Seoul, Korea). Chopped basalt fibers (BF) of 0.16 mm length and 13 ± 1 µm diameter were purchased from Deutsche Basalt Faser GmbH (Sangerhausen, Germany). The BF composition, according to the supplier, is presented in Table 1. Sulfuric acid (H₂SO₄) and sodium hydroxide (NaOH) was purchased from Scharlab (Barcelona, Spain). 3-Aminopropyltriethoxysilane (APTES) was purchased from Alfa-Aesar (Kandel, Germany).

Barium (II) nitrate (99.95%), scandium (III) nitrate hydrate (99.9%), iron (III) nitrate nonahydrate (98+%), citric acid (99+%), tetraethylorthosilicate (99%), sodium hydroxide (98%), 3-aminopropyl)triethoxysilane (APTES) (98%), and sodium alendronate (a source of AL) (97%) were purchased from Alfa Aesar (Lancashire, UK). Nitric acid was purchased from Sigma Aldrich (Burlington, MA, USA).
Precise concentrations of the metals in the salts were determined with inductively coupled plasma (ICP-OES, Agilent 720, Santa Clara, CA, USA). Deionized water was used in all experiments. Barium hexaferrite nanoplatelets partly substituted with Sc³⁺ (BSHF NPLs) were synthesized hydrothermally as previously described [9 (link)], followed by washing with Nitric acid and water. The obtained BSHF NPLs were colloidally stable in water after tuning the pH to pH = 2 with Nitric acid.
The BSHF NPLs showed a typical hexagonal plate-like shape with a magnetoplumbite crystal structure (Figure 1a). A selected-area electron diffraction (SAED) of the sample is shown in Figure S1 in the Supplementary Materials. The detailed structural analysis of BSHF NPLs is given in [10 (link)]. Figure 1 represents magnetic hysteresis (Figure 1a) with saturation magnetization of Ms~35 Am²kg⁻¹ and a significant coercivity of Hc = 97 kAm⁻¹ typical for hard magnetic materials such as barium hexaferrite (Figure 1b).

Adenosine 5′-triphosphate (ATP) disodium salt hydrate, Potassium hydroxide (KOH), phenol, DNA fish sperm, Curcumin, ribose, glucose, fructose, potassium chloride (KCl), glutaraldehyde (Glu), and ethanolamine (ETA) were bought from Merck, Darmstadt, Germany. (3-Aminopropyl) triethoxysilane (APTES) was obtained from ALFA AESAR, Lancashire, UK. Hydrochloric acid (HCl) was bought from VWR life science AMRESCO, Limerick, Ireland, while ethanol was acquired from Laurylab, Brindas, France. Sulfuric acid (H₂SO₄) and sodium phosphate dibasic (Na₂HPO₄, 12H₂O) were bought from Solvachim (Casablanca, Morocco).
The chemicals necessary for preparing the phosphate-buffered saline (0.01 M of PBS with 2.7 mM of KCl and 137 mM of NaCl, pH 7.4) were purchased from Merck, Germany, and all reagents used were of analytical grade.
PBS served as the washing buffer.
HPLC pure oligonucleotides in lyophilized powder form were supplied by Eurofins Genomics, The Ulis, France. Oligonucleotide sequences are detailed in Table 1. Stock solutions of synthetic oligonucleotides were created in ultra-pure water, divided into aliquots, and stored at −20 °C.

Bovine gelatin (G9382, type B) and human FN were purchased from Sigma-Aldrich (St. Louis, MO, USA). Trypsin (sequencing grade) was obtained from Promega (Madison, WI, USA). Trifluoroacetic acid (TFA) and acetonitrile (ACN) were purchased from Fisher Scientific (Fair Lawn, JN, USA). α-Cyano-4-hydroxycinnamic acid (CHCA) was purchased from Bruker (Billerica, MA, USA). 3-Aminopropyltriethoxysilane (APTES) was purchased from Alfa Aesar (Haverhill, MA, USA). An unmodified Anachip was purchased from Farfield (Sensors Ltd., Salford, UK). The four synthesized peptides were purified by Beijing Scilight Biotechnology Led. Co. (Beijing, China).

Gossypol was purchased from Sigma-Aldrich (Shanghai, China). Dimethylaminoethyl methacrylate (DMAEMA), ethylene glycol dimethacrylate (EGDMA), divinylbenzene (DVB), 1,1’-bi-2-naphthol, and 2,2-azobis(isobutyronitrile) (AIBN) were purchased from Adamas Reagent Co., Ltd. (Shanghai, China). Hexaphenol was purchased from WAKO Pure Chemical Industry, Ltd. (in Japan). Tetraethoxysilane (TEOS), ellagic acid, and quercetin were obtained from Adamas (Shanghai, China). Silica beads (0.5 μm average particle size) and (3-aminopropyl) triethoxysilane (APTES) were purchased from Alfa Aesar (Shanghai, China). Methanol, acetone, hydrochloric acid, acetic acid, and sodium hydroxide were all analytical grade and purchased from Tianjin Zhiyuan Chemical Co., Ltd. (Tianjin, China). Water was purified by a Millipore Milli-Q gradient system (Massachusetts, America) to HPLC grade.