Medium chain triglycerides

The Achyrocline satureioides plant (voucher specimen: number 308) was obtained from CPQBA-UNICAMP (São Paulo, Brazil). Egg-lecithin (Lipoid E-80) and medium chain triglycerides (MCT) were purchased from Lipoid GmbH (Ludwigshafen, Germany). Polysorbate 80 and vitamin E were supplied from Vetec Química Fina Ltda. (Rio de Janeiro, Brazil) and Alpha Química (Porto Alegre, Brazil), respectively. Quercetin, luteolin, and 3-O-methylQuercetin standards were obtained from Sigma (Steinheim, Germany), Alfa Aesar (Lancashire, United Kingdom), and Extrasynthese (Genay, France), respectively. Nile Red was purchased from Sigma (Steinheim, Germany). Porcine ears and esophagus were gently donated by Ouro do Sul Ltda. (Harmonia, Brazil). All solvents used were HPLC grade: methanol (J.T. Baker, Center Valley, USA), acetonitrile (Tedia Brasil, Rio de Janeiro, Brazil), and phosphoric acid (Merck, Darmstadt, Germany).

A. satureioides was acquired from Centro Pluridisciplinar de Pesquisas Químicas, Biológicas e Agrícolas (CPQBA) da Universidade Estadual de Campinas (São Paulo, Brazil), via a sample deposited in the herbarium with the number 308. Egg yolk lecithin and medium-chain triglycerides were purchased from Lipoid (Ludwigshafen, Germany), polysorbate 80 was acquired from Vetec (Rio de Janeiro, Brazil), and vitamin E was procured from Alpha Química (Cachoeirinha, Brazil). Ultra-fast liquid chromatography (UFLC) required the following reagents: methanol (J.T. Baker, Philipsburg, NJ, USA), acetonitrile (Tedia, Rio de Janeiro, Brazil), and phosphoric acid (Merck, Rio de Janeiro, Brazil). Carbopol^® Ultrez 20 was kindly donated by Lubrizol do Brasil Aditivos Ltd.a (São Paulo, Brazil). Albumin for enzyme-linked immunosorbent assay (ELISA) was purchased from Calbiochem (San Diego, CA, USA). Normal goat serum was obtained from R&D Systems (Minneapolis, MN, USA). Protease inhibitor cocktail was purchased from VWR Life Science (Radnor Township, PA, USA). Xylazine and ketamine were purchased from Ceva (Paulínea, Brazil). Ketoprofen was obtained from Merial (Paulínea, Brazil). Finally, thiopental was obtained from Cristália (Itapira, Brazil).