From each histological subtype, 1–2 representative tumor areas were identified on HE-stained sections, according to tumor histology. If normal testicular tissue samples were present, they were also marked. Sections were matched to the donor blocks (corresponding wax blocks). Tumor cores (3-mm diameter) were removed from the donor blocks using the multipurpose sampling tool Harris Uni-Core (Sigma-Aldrich) and inserted into the recipient master block. The recipient block was cut into 5-µm sections, and sections were transferred to FLEX IHC Microscope slides (cat. no. K8020; Dako, Glostrup, Denmark).
Harris uni core
Manufactured by Merck Group
Sourced in Germany
The Harris Uni-Core is a laboratory equipment designed for efficient core sampling and extraction. It provides a reliable and consistent method for obtaining uniform core samples from a variety of materials.
Automatically generated - may contain errors
15 protocols using harris uni core
1
Tissue Microarray Construction Protocol
2
Tissue Microarray Construction Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
According to tumor histology, one or two representative tumor areas were identified on H&E sections. Sections were matched to their corresponding wax blocks (the donor blocks), and 3-mm diameter cores of the tumor were removed from these donor blocks with the multipurpose sampling tool Harris Uni-Core (Sigma-Aldrich, Steinheim, Germany) and inserted into the recipient master block. The recipient block was cut into 5-μm sections, and the sections were transferred to coated slides.
3
Comprehensive Brain Region Dissection for RNA Extraction
4
Microfluidic Device for Single-Cell Culturing
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The microfluidic device (microfluidic chip, or “chip” for short) used in this study was designed by the Hasty lab [25 (link)]. It consists of a cell trapping chamber, five ports, a dial-a-wave (DAW) junction and a staggered herringbone mixer. The height of the trapping chamber is 3.5 μm allowing cells to grow in a single layer. For casting chips, poly-dimethylsiloxane (PDMS, Sylgard 184, Dow Corning) was mixed in 1:10 ratio (catalyst:base), degassed and cast against a master mold. The solution was cured at 80°C for 1 hr in a dry oven. The five ports were created by punching the PDMS monolith using a 0.5 mm puncher (Harris Uni-Core, Sigma-Aldrich). Chips were cleaned by sonication in methanol. Using UV/ozone treatment, a chip and a glass coverslip were exposed to UV light and ozone in a UVO cleaner (Jelight Company Inc.) for 3 min. Treated surfaces were brought into contact within 1 min and allowed to anneal at 80°C overnight to enhance bond strength [28 (link)]. They were stored at room temperature until subsequent experiments.
5
Germ Cell Tumor Tissue Sampling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
According to the tumor histology, one or two representative tumor areas from each histological subtype of germ cell tumor (from 1–6 cores from each tumor) were identified on haematoxylin- and eosin-stained sections. Samples from normal testicular tissue and germ cell neoplasia in situ were also marked, if present. Sections were matched to their corresponding wax blocks (the donor blocks), and 3-mm diameter cores of the tumor were removed from these donor blocks with the multipurpose sampling tool Harris Uni-Core (Sigma-Aldrich, Steinheim, Germany) and inserted into the recipient master block. The recipient block was cut into 5-μm sections that were transferred to coated slides.
6
Germ Cell Tumor Tissue Sampling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
According to tumor histology, one or two representative tumor areas from each histological subtype of germ cell tumor (from 1–6 cores from each tumor) were identified on haematoxylin and eosin stained sections. Samples from normal testicular tissue and germ cell neoplasia in situ were also marked, if present. Sections were matched to their corresponding wax blocks (the donor blocks), and 3-mm diameter cores of the tumor were removed from these donor blocks with the multipurpose sampling tool Harris Uni-Core (Sigma-Aldrich, Steinheim, Germany) and inserted into the recipient master block. The recipient block was cut into 5-µm sections that were transferred to coated slides.
7
Tissue microarray construction for germ cell tumors
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
According to the tumor histology, one or two representative tumor areas from each histological subtype (1–6 cores from each tumor) of germ cell tumor were identified on H&E sections. In case of mixed germ cell tumors, selected GCT histologies were sampled to isolate a specific histological pattern. Samples from normal testicular tissue were also marked, if present. Sections were matched to their corresponding wax blocks (the donor blocks), and 3-mm diameter cores of the tissues were removed from these donor blocks with the multipurpose sampling tool Harris Uni-Core (Sigma-Aldrich, Steinheim, Germany) and inserted into the recipient master block. The recipient block was cut into 5-μm sections and sections were transferred to coated slides. Tissue microarray construction and immunohistochemical staining was described in detail previously [4 (link)].
8
Fabrication of PDMS Microfluidic Channels
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Silicon flow channel masters were
produced by standard photolithography steps and deep reactive ion
etching. The polydimethylsiloxane (PDMS) flow channels were prepared
from a degassed mixture of 10:1 Sylgard 184 elastomer and curing agent
(Dow Corning Corp), which was cast onto the silicon master and cured
at 60 °C overnight. The flow channels were cut to size, and inlets
and outlets were punched using a 1 mm Ø punch (Harris Unicore,
Sigma-Aldrich). After bonding to the chip, a flow channel of 6000 ×
500 × 50 μm3 was prepared with a second channel
entering from the side.
produced by standard photolithography steps and deep reactive ion
etching. The polydimethylsiloxane (PDMS) flow channels were prepared
from a degassed mixture of 10:1 Sylgard 184 elastomer and curing agent
(Dow Corning Corp), which was cast onto the silicon master and cured
at 60 °C overnight. The flow channels were cut to size, and inlets
and outlets were punched using a 1 mm Ø punch (Harris Unicore,
Sigma-Aldrich). After bonding to the chip, a flow channel of 6000 ×
500 × 50 μm3 was prepared with a second channel
entering from the side.
9
Tissue Microarray Construction for Germ Cell Tumors
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
According to tumour histology, one or two representative tumour areas from each histological subtype (1–6 cores from each tumor) of germ cell tumour were identified on H&E sections. In case of mixed germ cell tumors, selected GCT histologies were sampled to isolate a specific histological pattern. Samples from non-neoplastic testicular tissue and germ cell neoplasia in situ were also marked, if present. Sections were matched to their corresponding wax blocks (the donor blocks), and 3-mm diameter cores of the tumour were removed from these donor blocks with the multipurpose sampling tool Harris Uni-Core (Sigma-Aldrich, Steinheim, Germany) and inserted into the recipient master block. The recipient block was cut into 4-μm sections that were transferred to coated slides.
10
Tissue Microarray Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
According to tumour histology, one or two representative tumour areas were identified on the hematoxylin and eosin sections. Sections were matched to their corresponding wax donor blocks, and 3-mm diameter cores of the tumour were removed from the donor blocks with the multipurpose sampling tool Harris Uni-Core (Sigma-Aldrich, Steinheim, Germany) and inserted into the recipient master block. The recipient block was cut into 5-μm sections, which were transferred to coated slides.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!