Materials used in this study were l -histidine hydrochloride, l -arginine, stearic acid, and hydrogen peroxide (30% w/v; Sangon Biotech, Shanghai, People’s Republic of China); polyethylenimine (PEI, branched, molecular weight 25 kDa), Lipofectamine™ 2000, l -buthionine-sulfoximine, 4′,6-diamidino-2-phenylindole dihydrochloride, l -cysteine hydrochloride monohydrate, ethidium bromide, dithiothreitol (DTT, Sigma-Aldrich, St Louis, MO, USA); a luciferase assay kit (Promega, Madison, WI, USA); pDNA (pGL3 and pEGFP) (Shanghai Innovation Biotechnology Co Ltd, Shanghai, People’s Republic of China); enhanced bicinchoninic acid protein assay kit (Beyotime, Nanjing, People’s Republic of China); Dulbecco’s Modified Eagle’s Medium (DMEM), fetal bovine serum, YOYO-1 (Y3601) and penicillin–streptomycin solution 5 kU/mL (Life Technologies, Carlsbad, CA, USA); a Cell Counting Kit-8 (CCK-8, Dojindo Molecular Technologies Inc, Nanjing, People’s Republic of China); and HEK293 cells and HeLa cells (Cell Culture Center of the Shanghai Institutes for Biological Sciences of the Chinese Academy of Sciences, Shanghai, People’s Republic of China). All other reagents were of analytical grade. All animal experiments were performed in accordance with the ethics and regulations of animal experiments of Second Military Medical University (Shanghai, People’s Republic of China).
Stearic acid
Manufactured by Sangon
Sourced in China
Stearic acid is a saturated fatty acid commonly used as a laboratory reagent. It has the chemical formula CH3(CH2)16COOH and is a waxy, white solid at room temperature. Stearic acid is primarily used as a starting material for the synthesis of other chemical compounds in various research and analytical applications.
Automatically generated - may contain errors
Lab products found in correlation
Hydrogen peroxide, by Sangon (2 mentions)
Luciferase assay kit, by Promega (2 mentions)
L arginine, by Sangon (2 mentions)
Enhanced bicinchoninic acid protein assay kit, by Beyotime (1 mentions)
Lipofectamine 2000, by Merck Group (1 mentions)
L histidine hydrochloride, by Sangon (1 mentions)
Yoyo 1 y3601, by Thermo Fisher Scientific (1 mentions)
L cysteine hydrochloride monohydrate, by Merck Group (1 mentions)
4 6 diamidino 2 phenylindole dihydrochloride, by Merck Group (1 mentions)
L buthionine sulfoximine, by Merck Group (1 mentions)
Penicillin streptomycin solution, by Thermo Fisher Scientific (1 mentions)
An enhanced bicinchoninic acid protein assay kit, by Beyotime (1 mentions)
Cell counting kit 8 cck 8, by Dojindo Laboratories (1 mentions)
Dithiothreitol (dtt), by Merck Group (1 mentions)
2 protocols using stearic acid
1
Optimizing Transfection Efficiency for Gene Delivery
2
Luminescent Nanoparticle-based Prostate Cancer Assay
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Enz, aminated GQDs, MAL-PEG-SCM (PEG molecular size: 2000), l -arginine, stearic acid, and hydrogen peroxide (30% w/v) were purchased from Sangon Biotech, Shanghai, China. Dithiothreitol (DTT) was purchased from Sigma-Aldrich (St Louis, MO, USA). A luciferase assay kit was gifted from Promega (Madison, WI, USA). An enhanced bicinchoninic acid protein assay kit was purchased from Beyotime (Nanjing, China). Dulbecco’s Modified Eagle Medium (DMEM), RPMI-1640 medium, foetal bovine serum (FBS), penicillin–streptomycin solution (5 KU/mL) was obtained from Life Technologies (Carlsbad, CA, USA). Cell Counting Kit-8 (CCK-8) was purchased from Dojindo Molecular Technologies Inc., Nanjing, China. LNCaP and C4-2B cells were obtained from Shanghai Cell Bank, Chinese Academy of Sciences, Shanghai, China. All other reagents were analytical grade. All animals were supplied from Department of Pharmacy of Fudan University and all animal experiments were performed in accordance with the ethics and regulations of animal experiments at Fudan University (Shanghai, China).
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