Wipi1

Manufactured by Merck Group

Sourced in Germany

WIPI1 is a laboratory equipment product manufactured by Merck Group. It is designed for use in various scientific research and analysis applications. The core function of WIPI1 is to perform tasks related to the detection and analysis of specific biomolecular or cellular components. The detailed specifications and intended use of this product are not available in this factual and unbiased description.

Automatically generated - may contain errors

Lab products found in correlation

Cycloheximide (chx), by Merck Group (1 mentions) Puromycin, by Merck Group (1 mentions) Rapamycin, by Enzo Life Sciences (1 mentions) Becn1, by Cell Signaling Technology (1 mentions) Phospho mtor, by Abcam (1 mentions) Pik3c3, by Cell Signaling Technology (1 mentions) Poly ubiquitin, by Agilent Technologies (1 mentions) Vimentin, by SCBT (1 mentions) Bafilomycin a1, by LC Laboratories (1 mentions) Erk1 2, by Cell Signaling Technology (1 mentions) P erk1 2, by Cell Signaling Technology (1 mentions) P70s6k thr389, by Cell Signaling Technology (1 mentions) Beclin 1, by Abcam (1 mentions) P70s6k, by Cell Signaling Technology (1 mentions) P mtor s 2448, by Abcam (1 mentions) Pi3k class 3, by Cell Signaling Technology (1 mentions)

2 protocols using wipi1

Autophagy-related Protein Detection

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Stock solutions of Bafilomycin A1 (LC Laboratories, B-1080), MG132 (Calbiochem, 474790), Cycloheximide (Sigma, 01810) and Rapamycin (Enzo, BML-A275-0025) were prepared in DMSO (Roth, A994.2). Stock solution of Canavanine (Santa Cruz Biotech, sc-202983A) and Puromycin (Sigma, P8833) was prepared in distilled H2O.
Antibody sources were as follows: for Actin (Sigma, A5060), BAG1 (Abcam, ab7976), BAG3 (Proteintech Group, 10599-1-AP), BECN1 (Cell Signaling, 3495), CTSD (Abcam, ab75852), DLP1 (BD Transduction Laboratories, 611113), LAMP2 (DSHB Biology, ABL-93), LC3B (Nanotools, 0260-100), LC3B (Sigma, L7543), OPA1 (BD Transduction Laboratories, 612607), Phospho mTOR (Abcam, ab109268), Puromycin (Millipore, MABE343), mTOR (Calbiochem, OP97), p62 (Progen, GP62-C), PIK3C3 (Cell Signaling, 4263), Poly-Ubiquitin (Dako, Z0458), RAB18 (Sigma, SAB4200173), Tubulin (Millipore, MAB1637), Tubulin (Sigma, T9026), TFEB (Proteintech Group, 13372-1-AP), Vimentin (SCBT, sc-373717), WIPI1 (Sigma, HPA007493).

Chakraborty D., Felzen V., Hiebel C., Stürner E., Perumal N., Manicam C., Sehn E., Grus F., Wolfrum U, & Behl C. (2019). Enhanced autophagic-lysosomal activity and increased BAG3-mediated selective macroautophagy as adaptive response of neuronal cells to chronic oxidative stress. Redox Biology, 24, 101181.

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Antibodies for Immunocytochemistry and Western Blot Analysis

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The antibodies used for immunocytochemistry as well as for western blot analysis in this study were as follows: ATG7 (8558, Cell Signaling Technologies, Danvers, MA, USA); BAG3 (ab47124, Abcam, Cambridge, UK); BCL2 (sc-492, Santa Cruz, Dallas, TX, USA); ERα (RM9101S0, Thermo Fischer Scientific, Waltham, MA, USA); ERK1/2 (9102, Cell Signaling Technologies); p-ERK1/2 (9106, Cell Signaling Technologies); LC3B (L7543, Sigma-Aldrich); mTOR (OP97, Millipore, Billerica, MA, USA); p-mTOR S2448 (ab51044, Abcam); NBR1 (00004077-M01, Abnova, Taipei, Taiwan); SQSTM1 (GP62-C, Progen, Heidelberg, Germany); PI3K Class III (4263, Cell Signaling Technologies); Tubulin (T9026, Sigma-Aldrich); WIPI1 (HPA007493, Sigma-Aldrich); Beclin1 (ab51031; Abcam); p70S6K (9202, Cell Signaling); p70S6K Thr389 (9206, Cell Signaling). Secondary antibodies were anti-mouse/rabbit/guinea pig antibodies conjugated to DyLight 488, 649 and Cy3 (immunofluorescence, Jackson ImmunoResearch, West Grove, PA, USA) or to HRP (immunoblotting, Jackson ImmunoResearch).

Felzen V., Hiebel C., Koziollek-Drechsler I., Reißig S., Wolfrum U., Kögel D., Brandts C., Behl C, & Morawe T. (2015). Estrogen receptor α regulates non-canonical autophagy that provides stress resistance to neuroblastoma and breast cancer cells and involves BAG3 function. Cell Death & Disease, 6(7), e1812-.

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