Dulbecco minimal essential medium

Salvianolic acid B, dihydrotanshinone I, salvianolic acid A, tanshinone II-A, amygdalin, adenosine, cordycepin, schizandrin, schisandrin B, schisantherin A, gypenoside XLIX and kaempferol were purchased from EFEBIO (Shanghai, China¹¹), and their structural information is shown in Supplementary Figure S1. 15-deoxy-Δ12,14-prostaglandin J2 (15d-PGJ2) was used as a positive control (Jin et al., 2016 (link)), and obtained from Abcam (Cambridge, United Kingdom). The structures of the above chemicals were unambiguously identified by ¹H NMR and MS spectra, and their purity was demonstrated to be 98% by HPLC-UV. Cell Counting Kit-8 (CCK8) detection kit was obtained from Beyotime (Shanghai, China). LX-2 cells and T6 cells were purchased from Cell Resource Center of Fudan IBS (Shanghai, China). These cells were incubated in Dulbecco minimal essential medium (Sigma, United States) with 10% fetal bovine serum (GIBCO, United States) and penicillin, streptomycin (GIBCO, United States) under a humidified atmosphere with 5% CO₂ at 37°C. The medium was renewed every 2 days.

Furin convertase inhibitor Decanoyl-Arg-Val-Lys-Arg-chloromethyl–ketone (Dec-RVKR-CMK) was purchased from Enzo Life Sciences, Inc. Human embryonic kidney 293 cells (HEK-293) were purchased from American Type Culture Collection (ATCC). The cells were cultured in Dulbecco minimal essential medium (Sigma–Aldrich) supplemented with 10% heat-inactivated bovine calf serum, penicillin (100 units/mL), and streptomycin (100 mg/mL) at 37°C in a humidified atmosphere of 5% CO2. Phoenix cells were purchased from Orbigen. The cells were cultured in Dulbecco minimal essential medium F12 (Sigma–Aldrich) supplemented with 10% heat-inactivated fetal bovine serum, penicillin (100 units/mL), and streptomycin (100 mg/mL) at 37°C in a humidified atmosphere of 5% CO2.