Cholesterol-modified AgomiR-378 and control oligos were ordered from RiboBio (Guangzhou, China). For the prevention study, C57BL/6 mice were fed an HFD, or an SD as control. During HFD feeding, AgomiR-378 was consecutively administered to the mice weekly by tail–vein injection (20 μg/g body weight) for 8 weeks. AgomiR-378 and control oligos were administered to 10 male mice, respectively. For treatment experiments, C57BL/6 mice were fed an HFD for 11 weeks to induce obesity, with mice fed an SD as control. Then, obese mice were treated weekly with AgomiR-378, or scrambled oligo as control, by tail–vein injection (20 μg/g body weight) for 4 consecutive weeks. After the last injection, mice underwent GTT and ITT and were then sacrificed for sampling.
Control oligos
Manufactured by RiboBio
Sourced in China
Control oligos are synthetic DNA or RNA sequences used as references in various molecular biology experiments. They serve as internal standards to validate the performance and accuracy of experimental procedures involving nucleic acids.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using control oligos
1
AgomiR-378 Modulates Obesity in Mice
2
Silencing CD59, IL-6, and STAT3 in Pancreatic Cancer
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Human CD59, IL-6 and STAT3 small-interfering RNAs (siRNAs) and control oligos were synthesized by RiboBio (Guangzhou, China). AsPC-1 and BxPC-3 cells were transfected with the siRNAs (50 nM) using Lipofectamine 3000 transfection reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol. The target sequences of the siRNAs used in this research were as follows: si-h-CD59 (5′-3′), GGTTACAAGTGTATAACAA; si-h-IL-6 (5′-3′), GGAGTTTGAGGTATACCTA; and si-h-STAT3 (5′-3′), CATCTGCCTAGATCGGCTA.
3
Targeted Knockdown of Key Regulators
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Specific siRNA oligos for human CHEK2, p53, and PRAS40 as well as control oligos were synthesized by RiboBio Co. (Guangzhou, China). The siRNAs (50-100 nM) were transfected using Lipofectamine 3000 transfection reagent (Invitrogen) according to the manufacturer's protocol. Control RNA (Ctrl-siN05815122147) was obtained from RiboBio Co. (Guangzhou, China).
The specific siRNA sequences were as follows:
CHEK2-specific siRNA-1, 5' GUAAGAAAGUAGCCAUAAA dTdT 3'; CHEK2-specific siRNA-2, 5' GGAAUAAACGCCUGAAAGA dTdT 3'; CHEK2-specific siRNA-3, 5' GAACAUACAGCAAGAAACA dTdT 3'; p53-specific siRNA, 5'-GGAAGACUCCAGUGGUAAUdTdT-3' PRAS40-specific siRNA, 5-CCAGAAGCUGAAGCGGAAAUAUUGA-3
The specific siRNA sequences were as follows:
CHEK2-specific siRNA-1, 5' GUAAGAAAGUAGCCAUAAA dTdT 3'; CHEK2-specific siRNA-2, 5' GGAAUAAACGCCUGAAAGA dTdT 3'; CHEK2-specific siRNA-3, 5' GAACAUACAGCAAGAAACA dTdT 3'; p53-specific siRNA, 5'-GGAAGACUCCAGUGGUAAUdTdT-3' PRAS40-specific siRNA, 5-CCAGAAGCUGAAGCGGAAAUAUUGA-3
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