Samples were prepared from 5‐μl aliquots of relevant aggregation, and reaction was absorbed onto a glow‐discharged carbon support film, washed twice using 100 μl of distilled water, and negatively stained with 2% (wt/vol) uranyl acetate. Grids were examined at ×100,000 to ×150,000 magnification, and images were examined with a JEM‐100CX electron microscope (JEOL, Tokyo, Japan, www.jeol.co.jp ) at 80 kV.
Jem 100 cx electron microscope
Manufactured by JEOL
Sourced in Japan, United States
The JEM-100 CX is a transmission electron microscope (TEM) manufactured by JEOL. It is designed to produce highly magnified images of small-scale structures and samples. The JEM-100 CX uses a beam of electrons to illuminate the specimen and create a magnified image that can be observed on a screen or recorded digitally.
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Lab products found in correlation
Ultramicrotome, by Leica (2 mentions)
Zetasizer nano series, by Malvern Panalytical (1 mentions)
Magellan 400 microscope, by Thermo Fisher Scientific (1 mentions)
Icp oes, by Agilent Technologies (1 mentions)
Bet analyzer, by Micromeritics (1 mentions)
Facscalibur, by BD (1 mentions)
Zetasizer, by Malvern Panalytical (1 mentions)
Bca protein assay kit, by Merck Group (1 mentions)
Anti cd63 antibody, by Santa Cruz Biotechnology (1 mentions)
Anti cd81 antibody, by Santa Cruz Biotechnology (1 mentions)
Carbon coated grid, by Electron Microscopy Sciences (1 mentions)
Versa 3d scanning electron microscope, by Thermo Fisher Scientific (1 mentions)
Zetasizer zs, by Malvern Panalytical (1 mentions)
V 630 spectrometer, by Jasco (1 mentions)
Pcs zetasizer nano zs series dts 1060, by Malvern Panalytical (1 mentions)
Nano zs series dts 1060, by Malvern Panalytical (1 mentions)
Uv 2450 spectrophotometer, by Shimadzu (1 mentions)
Alpha 1 2 ld plus, by Martin Christ (1 mentions)
Libra s80 spectrophotometer, by Harvard Bioscience (1 mentions)
33 protocols using jem 100 cx electron microscope
1
Negative Stain Electron Microscopy
2
Nanoparticle Characterization Techniques
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Transmission electron microscope (TEM) images were acquired on a JEM-100CX electron microscope (JEOL, Japan). Scanning electron microscope (SEM) images, element mapping images, and elements line scanning results were obtained on a field-emission Magellan 400 microscope (FEI Co.). Zeta potential and dynamic light scattering (DLS) measurements were conducted on a Zetasizer Nanoseries (Malvern Instruments, Malvern, UK). The pore size distribution and N2 adsorption-desorption isotherm of the nanoparticles were determined by Brunauer-Emmett-Teller (BET) analyzer (Micromeritics, ASAP 2020). The drug concentrations in plasma, urine, and feces samples were analyzed using liquid chromatography and mass spectrometry (LC-MS/MS, Agilent, Tokyo, Japan). The Si concentration measurement was conducted by an inductively coupled plasma optical emission spectrometer (ICP-OES, Agilent, Tokyo, Japan).
3
Exosome Surface Protein Characterization
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Isolated exosomes were analyzed according to their surface tetraspanin proteins using a modified protocol (Barranco et al., 2019 (link)). Conjugated antibodies against CD9, CD63, and CD81 were used to label the exosomes. 50 μL of Exosome MicroBeads was added to the exosome sample, vortexed and incubated for 1 h at room temperature. Following incubation exosome bound beads were further washed in PBS/1% BSA, blocked with 10% BSA, and stained with Anti-CD9 [CD9 (Santa Cruz Biotechnology, (C-4):sc-13118) conjugated with AlexaFlour@488, Anti-CD63 Antibody (Santa Cruz Biotechnology, MX-49.129.5: sc-5275) conjugated with AlexaFlour@647, and Anti-CD81 Antibody (Santa Cruz Biotechnology, (1.3.3.22): sc-7637] conjugated with AlexaFlour@546, all in concentration 1 µg/mL. The stained sample was incubated for 1 h at room temperature then washed and resuspended in FACS buffer for further analysis using BD FACS Calibur.
The size and the size distribution of both Ex and LUT-Ex were determined using Zetasizer (Malvern Instruments, UK) after their appropriate dilution with water. Additionally, their morphology was examined using transmission electron microscopy (TEM) (Jeol, JEM-100 CX electron microscope, Tokyo, Japan) after being stained with 1% w/v phosphotungstic acid. Their total protein content was quantified using the BCA protein assay kit (Sigma-Aldrich, USA).
The size and the size distribution of both Ex and LUT-Ex were determined using Zetasizer (Malvern Instruments, UK) after their appropriate dilution with water. Additionally, their morphology was examined using transmission electron microscopy (TEM) (Jeol, JEM-100 CX electron microscope, Tokyo, Japan) after being stained with 1% w/v phosphotungstic acid. Their total protein content was quantified using the BCA protein assay kit (Sigma-Aldrich, USA).
4
TEM Imaging of Biological Specimens
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We performed the TEM detection according to the methods described by Wang et al. [19 (link)]. Three specimens in each group were cut into small pieces, 1 mm × 1 mm × 1 mm. These pieces were washed with sodium cacodylate buffer, fixed in 2.5% glutaraldehyde, dehydrated with a graded series of ethanol, sectioned with an ultramicrotome (Leica, Japan) and stained with saturated uranium acetate. TEM detections were performed by one of the authors using a JEM 100CX electron microscope (JEOL, Tokyo, Japan).
5
Elucidating SUL-LPS Morphology via TEM
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The morphology of SUL-LPS was elucidated using the transmission electron microscope (TEM; JEM-100 CX Electron Microscope, JEOL, Japan) at a high voltage of 80 kV. One drop of freshly diluted dispersions was placed on a carbon-coated copper grid, leaving a thin film. Dispersions were subsequently stained with uranyl acetate solution for 30 s, followed by air-drying prior to imaging.
6
Electron Microscopy Analysis of Transfected Cells
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48 h post-transfection (p.t.) with pBI-EGFP or pBI-EGFP/3C, cells were trypsinized, pelleted, washed with PBS, and resuspended in a fixative solution (0.2 M cacodylic acid-NaOH buffer, pH 7.5, and 2% glutaraldehyde). Sections were cut on an LKB III ultratome (Sweden) and examined under a JEM-100CX electron microscope (JEOL, Japan) at accelerating voltage of 80 kV.
7
Transmission Electron Microscopy of EVs
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Diluted EVs were adsorbed onto glow-discharged, carbon-coated copper grids (Quantifoil) for 2 min before manually removing excess liquid by filter paper. Grids were negatively stained in 2% uranyl acetate for 2 min and air-dried prior to imaging. All images were taken on a JEOL JEM 100CX electron microscope at 100 kV.
8
Ultrastructural Analysis of SW620 Cells
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SW620 cells were cultured in serum-free medium for 2, 4 or 8 h. The cells were fixed with 2% paraformaldehyde and 2% glutaraldehyde in 0.1 ml/L phosphate buffer (pH 7.4) followed by 1% osmium tetroxide. After dehydration, thin sections were stained with uranyl acetate and lead citrate and observed using a JEM 100 CX electron microscope (JEOL, Peabody, NY, USA).
9
TEM Imaging of β2m Aggregates
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TEM images were
obtained on a JEOL JEM-100CX electron microscope operated at 100 keV.
Before analysis, a solution containing β2m aggregates was centrifuged
(12500 rpm) for 5 min and decanted. The solid material was then suspended
in 1 mL of deionized water, applied to carbon-coated grids (Electron
Microscopy Sciences, Hatfield, PA), stained with 1% phosphotungstic
acid (pH 7.4), air-dried overnight, and then analyzed.
obtained on a JEOL JEM-100CX electron microscope operated at 100 keV.
Before analysis, a solution containing β2m aggregates was centrifuged
(12500 rpm) for 5 min and decanted. The solid material was then suspended
in 1 mL of deionized water, applied to carbon-coated grids (Electron
Microscopy Sciences, Hatfield, PA), stained with 1% phosphotungstic
acid (pH 7.4), air-dried overnight, and then analyzed.
10
Ultrastructural Preparation of Cells
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The collected cells were fixed with 2.5% glutaraldehyde at 4 °C overnight and washed three times. Then, the cells were postfixed in 1% osmic acid at 4 °C for 2 h and dehydrated with a series of ethanol solutions. After being embedded in Spurr’s epoxy resin, the samples were cut into 50–60 nm ultrathin sections. Finally, the images were photographed by a JEOL JEM-100CX electron microscope.
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