For intracellular staining, the cultured cells were subjected to the following treatment. Briefly, the cell surfaces were initially stained with anti-mCD8a Ab and anti-mouse/humanCD44 Ab for 30 min at 4 °C. The cells were then fixed with 4% paraformaldehyde for 10 min at room temperature and permeabilized with 0.5% (v/v) Triton X-100 for 10 min at 4 °C. Finally, all samples were incubated with anti-cytokine Ab or cytolytic granule Ab for 30 min at 4 °C.
Mem non essential amino acids
MEM non-essential amino acids is a laboratory product manufactured by Nacalai Tesque. It serves as a source of non-essential amino acids for cell culture media formulations.
Lab products found in correlation
7 protocols using mem non essential amino acids
Cytokine and Cytolytic Granule Production Assay
For intracellular staining, the cultured cells were subjected to the following treatment. Briefly, the cell surfaces were initially stained with anti-mCD8a Ab and anti-mouse/humanCD44 Ab for 30 min at 4 °C. The cells were then fixed with 4% paraformaldehyde for 10 min at room temperature and permeabilized with 0.5% (v/v) Triton X-100 for 10 min at 4 °C. Finally, all samples were incubated with anti-cytokine Ab or cytolytic granule Ab for 30 min at 4 °C.
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