Field-emission scanning electron microscope (FE-SEM) images were obtained using JEOL JSM 7800 operated at 0.6 kV. Transmission electron microscopy (TEM) images were obtained with JEOL 2100 operated at 200 kV. The samples for TEM measurements were prepared by dispersing and drying the powder samples-ethanol dispersion on carbon film on a Cu grid. Nitrogen adsorption-desorption isotherms were measured at 77 K by using a Micromeritics Tristar II system. Samples were degassed at 453 K overnight on a vacuum line prior to the Nitrogen sorption analysis. The total pore volume was calculated from the amount adsorbed at a maximum relative pressure (P/P0) of 0.99. The Barrette-Joynere-Halanda (BJH) method was utilised to calculate the entrance size from the desorption branches of the isotherms, and the Brunauere-Emmette-Teller (BET) method was utilised to calculate the specific surface areas. Zeta potential measurements were conducted on a Zetasizer Nano ZS analyser (Malvern Instruments, Worcestershire, UK).
Zetasizer nano zs analyser
Manufactured by Malvern Panalytical
Sourced in United Kingdom
The Zetasizer Nano ZS analyser is a dynamic light scattering (DLS) instrument used to measure the size and zeta potential of particles, molecules, and colloids in liquid samples. It provides data on the hydrodynamic size, size distribution, and zeta potential of a wide range of materials, including nanoparticles, proteins, polymers, and emulsions.
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Lab products found in correlation
7 protocols using zetasizer nano zs analyser
1
Characterization of Nanomaterial Morphology and Surface Properties
2
Dynamic Light Scattering of aNP
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Obtained aNP formulations in PBS were filtered through a 0.22 µm PES syringe filter and analysed by DLS on a Malvern Zetasizer Nano ZS analyser. Values are reported as the mean number average size distribution.
3
Dynamic Light Scattering of NISTmAb
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A Malvern ZetaSizer Nano-ZS analyser (Malvern Instruments, UK) was used for dynamic light scattering (DLS) measurements. The 4 mW HeNe laser was set at λ = 633 nm with the detector angle at 173° for backscattering measurements. The NISTmAb samples were adjusted with 25 mmol/L His-buffer (pH 6.0) to give a concentration of 1 mg/mL. His-buffer was filtered through a Millipore Millex-GV 0.22 μm PVDF filter. DLS spectra were acquired in a disposable polystyrene ultra-micro cuvette at room temperature. Each spectrum was collected over three runs consisting of twelve ten second scans. The three runs were then averaged.
4
Characterization of Synthesized AuNPs
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A Perkin Elmer Lambda 900 UV/VIS/NIR spectrometer was used to measure the absorbance and observe the formation and stability of the AuNPs. A Zetasizer Nano ZS analyser (Malvern Instruments, Worcestershire, UK) was used to measure the hydrodynamic particle size and zeta-potential of the nanoparticles. Six replicates were measured for each data point. For gel electrophoresis, an aqueous solution of 0.8% (w/v) agarose gel was prepared and immersed in Tris–borate–EDTA buffer. The gel of 7 × 7 cm size and 1 cm thickness was run on a horizontal electrophoresis system with an electrode spacing of 15 cm (Mini-Sub Cell GT System, Bio-Rad, Hercules, CA, USA). After electrophoresis at 200 V, the AuNPs were separated according to their different electric charge (i.e., plain AuNPs, conjugated AuNPs, conjugated AuNPs + antigen), and the gel stained for the visualisation of the bands.
5
Characterization of Metal Oxide Nanoparticles
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The Fe2O3 (4–8 nm), Al2O3 (40 nm), and Cu (40 nm) nanoparticles used in this study were purchased from Plasma Chem (Berlin, Germany) and characterised elsewhere (31 (link)). Briefly, the size and morphology of nanoparticles were observed with a transmission electron microscope (TEM) JEM-1400 (Jeol, Tokyo, Japan) at 80 kV and 40000x magnification. Hydrodynamic radius determined with a Zetasizer Nano ZS analyser (Malvern Instruments Ltd, Malvern, UK) in extensively sonicated water suspensions of nanoparticles (25–50 μg/mL) showed much higher average diameter of Fe2O3, Al2O3, and Cu nanoparticles than declared (16±5 nm, 59±8 nm, and 51±4 nm, respectively), most likely due to agglomeration in water (32 (link)).
6
Synthesis and Characterization of Colloidal Gold Nanoparticles
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A Perkin Elmer Lambda 900 UV/VIS/NIR Spectrometer and Zetasizer Nano ZS analyser (Malvern Instruments, Worcestershire, UK) were used to measure the absorbance, hydrodynamic particle size and zeta potentials of the nanoparticles and to observe the formation and stability of GNPs. The diameter, Zeta Potential and Surface Plasmon Resonance of the colloidal gold diagnostic reagent solutions were measured immediately after the Aspergillus niger supernatant liquids were added into the Chloroauric acid and HEPES buffer solution.
7
Characterization of Synthesized GNPs
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A Perkin Elmer Lambda 900 UV/VIS/NIR Spectrometer was used to measure the absorbance and to observe the formation and stability of GNPs. A Zetasizer Nano ZS analyser (Malvern Instruments, Worcestershire, UK) was used to measure the hydrodynamic particle size and zeta potentials of the nanoparticles. Six replicates have been measured for each data point.
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