SEM–EDS technique characterizes the morphology and texture of rock grains in sub-micrometer spatial resolution with semi-quantitative elemental microanalysis. Small grains from the rocks were transferred on a double carbon tape on an aluminium stub and coated with 10 nm of osmium tetroxide (OsO4) (Filgen, Japan) before imaging. Samples were examined using a Hitachi S530 SEM (HITACHI, Japan) with emission current adjusted to 60 µA and voltage to 20 kV. Quartz PCI version 8 image management system (QUARTZ, Canada) was used for image acquisition. Elemental analysis of rock grains by EDS was performed using a JEOL JSM-6610LV SEM (JEOL, Japan) and equipped with the X-Max EDS system (Oxford Instruments, High Wycombe, UK). INCA software (Oxford Instruments, High Wycombe, UK) was used for data acquisition and processing.
Jsm 6610lv sem
Manufactured by JEOL
Sourced in Japan, United States
The JEOL JSM-6610LV is a Scanning Electron Microscope (SEM) that provides high-resolution imaging of a wide range of samples. It has a large chamber capacity and can accommodate a variety of specimen sizes and types. The JSM-6610LV utilizes a Tungsten filament electron source and delivers stable, high-quality electron beams for imaging.
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Lab products found in correlation
Avance 400 mhz, by Bruker (2 mentions)
4 benzyl chloride functionalized silica gel, by Merck Group (2 mentions)
16f model, by PerkinElmer (2 mentions)
X max eds system, by Oxford Instruments (1 mentions)
Inca software, by Oxford Instruments (1 mentions)
S 530 sem, by Hitachi (1 mentions)
Ems 550x, by Electron Microscopy Sciences (1 mentions)
Glutaraldehyde, by Merck Group (1 mentions)
Atr ftir spectrometer, by PerkinElmer (1 mentions)
Uatr two, by PerkinElmer (1 mentions)
Jfc 1600 auto fine coater, by JEOL (1 mentions)
Q150r es, by Quorum Technologies (1 mentions)
16 protocols using jsm 6610lv sem
1
Characterizing Rock Grains through SEM-EDS
2
Synthesis and Characterization of Solid-Supported Palladium Complexes
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Example 1
Materials for the synthesis of the solid-supported ligand and palladium complex were purchased from Sigma-Aldrich and were used as received. All solvents used in the synthesis were distilled before their use. 4-Benzyl chloride functionalized silica gel (200-400 mesh, extent of labelling: 1.2 mmol/g loading) was purchased from Sigma-Aldrich.
Solid state NMR spectral data was recorded using CP-MAS on a Bruker Avance 400 MHz machine. IR spectra were recorded in wavenumbers (cm−1) using FT-IR spectrometer (Perkin-Elmer 16F model). Elemental analyses were performed on Perkin Elmer Series 11 (CHNS/O) Analyzer 2400. Palladium loading was estimated using inductively coupled plasma mass spectrometer, X-series 2 ICP-MS, thermos scientific. Thermal stability of the solid-supported ligands and complexes were established using thermogravimetric (TG) (Perkin-Elmer TGA 7, US) analysis at a heating rate of 10° C. min−1 through to 700° C. under nitrogen atmosphere. The morphology of the supports, solid-supported ligands and solid-supported complexes were studied using scanning electron microscope, JEOL JSM6610LV SEM.
3
Synthesis and Characterization of Solid-Supported Palladium Complexes
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Example 1
Materials for the synthesis of the solid-supported ligand and palladium complex were purchased from Sigma-Aldrich and were used as received. All solvents used in the synthesis were distilled before their use. 4-Benzyl chloride functionalized silica gel (200-400 mesh, extent of labelling: 1.2 mmol/g loading) was purchased from Sigma-Aldrich.
Solid state NMR spectral data was recorded using CP-MAS on a Bruker Avance 400 MHz machine. IR spectra were recorded in wavenumbers (cm−1) using FT-IR spectrometer (Perkin-Elmer 16F model). Elemental analyses were performed on Perkin Elmer Series 11 (CHNS/O) Analyzer 2400. Palladium loading was estimated using inductively coupled plasma mass spectrometer, X-series 2 ICP-MS, thermos scientific. Thermal stability of the solid-supported ligands and complexes were established using thermogravimetric (TG) (Perkin-Elmer TGA 7, US) analysis at a heating rate of 10° C. min−1 through to 700° C. under nitrogen atmosphere. The morphology of the supports, solid-supported ligands and solid-supported complexes were studied using scanning electron microscope, JEOL JSM6610LV SEM.
4
Characterization of 3D-Printed Microfluidic Surfaces
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The channel geometries fabricated by 3-D printing and photolithography were coated with platinum for 8 min using an Emitech K550X (Emitech Inc., Fall River, MA, USA) sputter coater to prepare surfaces. Objects were scanned by a Wyko Hi Res optical profiler (Bruker Nano, Inc., Tuscon, AZ, USA) with a step size of 30 μm. The surface profiles and surface roughness (root-mean-square height, RMS) were analyzed using MountainsMap software (v8, Digital Surf, Besançon, France). Surface roughness was obtained at two regions: a 0.4 × 1.6-mm rectangular region on the floor of the main channels and a 1.6 × 1.6-mm square region on the platform surfaces (adjacent to the channels). Scanning electron microscopy (SEM) imaging was performed using a JEOL JSM-6610LV SEM at the Shared Instrumentation Facility of Louisiana State University. SEM images were acquired at 15 kV, with a working distance ~45 mm and 60-× magnification.
5
Synthesis of Cross-linked PVA Particles
6
Fracture Surface Microstructure Analysis
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After disassembly, the microstructure of the fracture surfaces was observed through SEM. Surfaces were coated with gold using a sputter coater (EMS550X, Electron Microscopy Sciences, Hatfield, PA, USA) under a vacuum of 10−1 mbar, at 25 mA for 2 min. A high-performance JSM-6610LV SEM (JEOL Ltd., Tokyo, Japan) was employed to capture images, at an acceleration voltage of 15 kV.
7
Chitosan Microspheres for G10E Delivery
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Chitosan microspheres loaded with G10E were prepared by emulsion crosslinking method as previously described (Jose et al., 2013 (link); Pichayakorn and Boonme, 2013 (link)) with minor modifications. Briefly, chitosan solution (3% w/w) was prepared by dissolving chitosan in 2% aqueous glacial acetic acid as aqueous phase. G10E peptides were mixed in this solution. Oil phase was paraffin oil containing 5% Span80. The aqueous phase was gradually added into the oil phase and continuously mixed at 1,000 rpm for 2h to form water-in-oil (w/o) emulsion. Subsequently, genipin was added into the mixture and mixed further for 2 h. The obtained microspheres were then separated by centrifugation at 3,000 rpm and the sediment was washed thrice with petroleum ether, isopropanol and deionized water and finally dried using a freeze dryer (CHRIST ALPHA 1-4 LSC, Germany) (Figure 1 ).
G10E-CS microspheres were characterized for morphological shape by a scanning electron microscope (JSM6610LV SEM, JEOL Ltd., Japan) as previously described (Wang et al., 2005 (link)). The samples were dusted on an adhesive carbon tape and coated with a thin layer of gold. Samples were then imaged at magnification of 15,000. The particle size and zeta potential of chitosan microspheres was determined by dynamic light scattering on a Zetasizer NanoZS (Malvern, UK). Measurements were carried out in triplicate.
G10E-CS microspheres were characterized for morphological shape by a scanning electron microscope (JSM6610LV SEM, JEOL Ltd., Japan) as previously described (Wang et al., 2005 (link)). The samples were dusted on an adhesive carbon tape and coated with a thin layer of gold. Samples were then imaged at magnification of 15,000. The particle size and zeta potential of chitosan microspheres was determined by dynamic light scattering on a Zetasizer NanoZS (Malvern, UK). Measurements were carried out in triplicate.
8
Observation of Bacterial Adhesion by SEM
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The method of Kubota et al. (2008 (link)) was followed, with some modifications, to observe by scanning electron microscopy (SEM) the cells adhered to the stainless steel coupons. Briefly, the latter were rinsed twice in PBS and then fixed in 2.5% glutaraldehyde (Sigma-Aldrich) in PBS for 16 h at room temperature. The fixed bacteria were then dehydrated using a graded series of acetone solutions (50–100% v/v), and the coupons dried with CO2 using a CPD-030 critical point dryer (Bal-Tec AG, Balzers, Liechtenstein). They were then coated with gold using a SCD 004 Sputtering Coater (Bal-Tec AG, Balzers, Liechtenstein) and observed using a JSM-6610LV SEM (JEOL USA, Inc, Peabody, MA, USA).
9
Characterization of Nano-Hydroxyapatite Composites
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FTIR spectra of the nano-hydroxyapatites and composite hydrogels were obtained from a Perkin Elmer, UATR two, ATR-FTIR spectrometer (Beaconsfield, Bucks, UK) in the wavelength range of between 4000–400 cm−1. Powder X-ray diffraction profile of hydroxyapatites and composite hydrogels was studied with Rigaku diffractometer, with Cu-Kα radiation at a voltage of 40 kV, current of 40 mA, and a scan rate of 0.02° s−1. The SEM/EDS (energy dispersive spectroscopy) analysis for nHA was carried out using the JEOL 6460LV scanning electron microscope at a voltage of 10 kV. The micro-morphology of composites were observed at a voltage 5 kV with a JEOL JSM-6610LV SEM. The samples were coated with gold by a sputter coater for excellent conductivity. Further gold coated hydrogel samples were air dried. The size and shape of the nHA-CS and nHA-MS nanoparticles were calculated by using the FEI Technai G2 20S-TWIN, USA transmission electron microscopy (TEM).
10
Scanning Electron Microscopy of Avian Blood
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Samples of venous blood from the birds were collected from the wing vein in 5 ml syringe using EDTA as anticoagulant. Few drops of blood were transferred into siliconized centrifuge tubes, equal amount of 5% glutaraldehyde was added and the blood was fixed for 1 h. The mix was spun at 3000 rpm for 30 min. The plasma was decanted and buffy coat was taken out in another tube. The blood cells were washed three times with 0.1 M phosphate buffer solution (pH 7.4) by centrifuging at 2000 rpm for 2 min. These samples of fixed cells were processed in SEM laboratory facility, GBPUAT, Pantnagar. There the cells were resuspended in distilled water and repeated washings were performed. The film of the blood cells was made on a clean circular glass coverslip. The blood film was coated with gold sputter coating using JFC-1600 Auto Fine Coater and observed under JEOL JSM-6610 LV SEM.
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