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Cd38 apc

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CD38-APC is a fluorescent-labeled antibody used in flow cytometry applications to detect and analyze CD38-expressing cells. It is a core reagent for immunophenotyping and research purposes.

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Lab products found in correlation

Cd4 apcef780, by Thermo Fisher Scientific (2 mentions) Lsrfortessa analyzer, by BD (2 mentions) Cd45ro fitc, by Thermo Fisher Scientific (2 mentions) Cd45ra percp cy5, by BioLegend (2 mentions) Cd8 bv605, by BioLegend (2 mentions) Cd28 pe cy7, by BioLegend (2 mentions) Live dead fixable blue dead cell stain, by Thermo Fisher Scientific (2 mentions) Cd3 v500, by BD (2 mentions) Cd45 fitc, by Thermo Fisher Scientific (2 mentions) Cd34 pe cy7, by Thermo Fisher Scientific (2 mentions) Ki67 bv786, by BD (1 mentions) Cd123 apc, by Thermo Fisher Scientific (1 mentions) Cd90 percpcy5, by Thermo Fisher Scientific (1 mentions) Cd47 apc, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 conjugated goat anti rabbit antibody, by Thermo Fisher Scientific (1 mentions) Cd38 pe, by Thermo Fisher Scientific (1 mentions) Igd fitc, by Thermo Fisher Scientific (1 mentions) Pd1 bv510, by BD (1 mentions) Cd3 percp, by BD (1 mentions) Cd4 bv510, by BD (1 mentions)

6 protocols using cd38 apc

1

Phenotypic Analysis of PBMCs

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Peripheral mononuclear cells (PBMCs) were isolated through Ficoll-Paque density gradient. 2×106 PBMCs were stained for phenotypic analysis with the following fluorochrome-conjugated
antibodies: CD3-V500 (BD Biosciences), CD4-APCeF780 (eBiosciences), CD8-BV605 (Biolegend), CD28-PE-Cy7 (Biolegend), CD38-APC (eBiosciences), CD45RO-FITC (eBiosciences), HLA-DR-AF700 (eBiosciences), CD45RA-PerCP-Cy5.5 (Biolegend). Viability was determined with Live/Dead fixable blue dead cell stain (Invitrogen). Intracellular stain was performed with FoxP3-Pacific Blue (eBiosciences) and Ki-67-BV786 (BD Biosciences) following the eBiosciences Fixation/Permeabilization protocol (Invitrogen). Samples were analyzed on a BD LSRFortessa analyzer (BD Biosciences). Flow cytometry data analysis was done with FlowJo V10, LLC Software.
Castro-Rojas C.M., Godarova A., Shi T., Hummel S.A., Shields A., Tremblay S., Alloway R.R., Jordan M.B., Woodle E.S, & Hildeman D.A. (2020). mTOR Inhibitor Therapy Diminishes Circulating CD8+ CD28− Effector Memory T Cells and Improves Allograft Inflammation in Belatacept-Refractory Renal Allograft Rejection. Transplantation, 104(5), 1058-1069.
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2

Isolation and Characterization of CD34+ Cells

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The umbilical cord blood was collected from normal full-term delivery after obtaining informed consent from the mothers as a donation for banking, and only cord blood samples not appropriate for banking (< 100 ml) were used in our experiments. The procedures of isolation and characterization of CD34+ cells were performed as we earlier described in Zhao’s article [7 (link)]. The phenotypic characterization on CD34+ cells was assayed using the flow cytometer for CD34-PCY7, CD45-FITC, and CD38-APC (eBioscience, San Diego, USA). The percentages of CD34+ cells and CD34+CD38− cells were 0.952 ± 0.025 and 0.105 ± 0.070 for each cord blood unit (mean with SD, n = 4).
Li Q., Zhao D., Chen Q., Luo M., Huang J., Yang C., Wang F., Li W, & Liu T. (2019). Wharton’s jelly mesenchymal stem cell-based or umbilical vein endothelial cell-based serum-free coculture with cytokines supports the ex vivo expansion/maintenance of cord blood hematopoietic stem/progenitor cells. Stem Cell Research & Therapy, 10, 376.
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3

Quantifying SIRT1 Expression in CD34+ Cells

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CD34+ cells were labeled with CD34-PeCy7, Lin-APC-Cy7 (including CD2, CD3, CD7, CD10, CD19, and CD235a), and CD38-APC or CD38-PE, CD90-PercpCy5.5, CD123-APC, or CD47-APC (eBioscience) antibodies; fixed and permeabilized (Cytofix/Cytoperm Kit, Beckman Coulter); labeled with rabbit anti-human SIRT1 (E1104-1, Millipore) and Alexa Fluor 488-conjugated goat anti-rabbit antibodies (Invitrogen); and analyzed by flow cytometry. Data were analyzed using FlowJo software (version 8.5.2; TreeStar).
Li L., Osdal T., Ho Y., Chun S., McDonald T., Agarwal P., Lin A., Chu S., Qi J., Li L., Hsieh Y.T., Santos C.D., Yuan H., Ha T.Q., Popa M., Hovland R., Bruserud Ø., Gjertsen B.T., Kuo Y.H., Chen W., Lain S., McCormack E, & Bhatia R. (2014). SIRT1 Activation by a c-MYC Oncogenic Network Promotes the Maintenance and Drug Resistance of Human FLT3-ITD Acute Myeloid Leukemia Stem Cells. Cell stem cell, 15(4), 431-446.
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4

Comprehensive Immune Profiling of Whole Blood Samples

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To determine the NK cells and lymphocyte subsets in patients, heparin‐anticoagulated whole blood samples were collected and stained with (1) CD3‐PerCP (BD Biosciences, San Jose, CA,), CD4‐BV510 (BD Biosciences, San Jose, CA), CD8‐APC (BD Biosciences, San Jose, CA), CD45RO‐BV421 (BioLegend, San Diego, CA), and CCR7‐PE (BD Biosciences, San Jose, CA); (2) CD4‐APC‐Cy7 (eBioscience, San Diego, CA), CXCR5‐APC (eBioscience, San Diego, CA), TIM‐3‐PerCP (eBioscience, San Diego, CA), TIGIT‐FITC (eBioscience, San Diego, CA), CD226‐PE‐Cy7 (eBioscience, San Diego, CA), PD‐1‐BV510 (BD Biosciences, San Jose, CA), and ICOS‐BV421 (BD Biosciences, San Jose, CA); (3) CD19‐PE (eBioscience, San Diego, CA), CD27‐PE‐Cy7 (eBioscience, San Diego, CA), CD38‐APC (eBioscience, San Diego, CA), CD24‐APC‐Cy7 (eBioscience, San Diego, CA), IgM‐BV421 (eBioscience, San Diego, CA,), and IgD‐FITC (eBioscience, San Diego, CA); and (4) CD3‐FITC (BD Biosciences, San Jose, CA) and CD16 + CD56‐PE (BD Biosciences, San Jose, CA). Cell counting was performed with absolute counting tubes with a certain number of beads (BD Biosciences, San Jose, CA). The samples were measured using FACS Canto II (BD Biosciences, San Jose, CA). Gating analysis was performed with FlowJo V10 (BD Biosciences, San Jose, CA).
Yan L., Cai B., Li Y., Wang M., An Y., Deng R., Li D., Wang L., Xu H., Gao X, & Wang L. (2020). Dynamics of NK, CD8 and Tfh cell mediated the production of cytokines and antiviral antibodies in Chinese patients with moderate COVID‐19. Journal of Cellular and Molecular Medicine, 24(24), 14270-14279.
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5

Enumeration of Hematopoietic Stem Cells

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At day 10, we harvested the nonadherent and adherent cells from five different culture conditions, and the total nucleated cells (TNCs) counted via trypan blue staining method. Flow cytometry staining was performed with CD34-PECY7, CD45-FITC, and CD38-APC (eBioscience, San Diego, USA) antibodies, then samples were analyzed using a FACScan flow cytometer (Beckman Coulter, USA). For each sample, at least 10,000 events were recorded. The isotype antibodies were used to determine the level of nonspecific binding.
Li Q., Zhao D., Chen Q., Luo M., Huang J., Yang C., Wang F., Li W, & Liu T. (2019). Wharton’s jelly mesenchymal stem cell-based or umbilical vein endothelial cell-based serum-free coculture with cytokines supports the ex vivo expansion/maintenance of cord blood hematopoietic stem/progenitor cells. Stem Cell Research & Therapy, 10, 376.
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6

Allogeneic T cell proliferation assay

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Donor and recipient PBMCs were thawed and rested for 1 hour in media with DNase I (Roche). For stimulators, APCs were isolated from donor PBMCs with CD3 MicroBeads (Miltenyi Biotec). For responders, recipient PBMCs were stained with CellTrace™ Violet Cell proliferation dye (ThermoFisher Scientific). 3×104 recipient responder PBMCs were cultured for 5 days in serum-free AIM V media with 8×104 isolated stimulator APCs from donor PBMCs. Cells were then washed with PBS, blocked with Fc Block, stained for surface markers CD3-V500 (BD Biosciences), CD4-APCeF780 (eBiosciences), CD8-BV605 (Biolegend), CD28-PE-Cy7 (Biolegend), CD38-APC (eBiosciences), CD45RO-FITC (eBiosciences), (eBiosciences), and CD45RA-PerCP-Cy5.5 (Biolegend). Viability was determined with Live/Dead fixable blue dead cell stain (Invitrogen). Samples were analyzed on a BD LSRFortessa analyzer (BD Biosciences). Flow cytometry data analysis was done with FlowJo V10, LLC Software.
Castro-Rojas C.M., Godarova A., Shi T., Hummel S.A., Shields A., Tremblay S., Alloway R.R., Jordan M.B., Woodle E.S, & Hildeman D.A. (2020). mTOR Inhibitor Therapy Diminishes Circulating CD8+ CD28− Effector Memory T Cells and Improves Allograft Inflammation in Belatacept-Refractory Renal Allograft Rejection. Transplantation, 104(5), 1058-1069.
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