Total DNA was extracted using the GenUP gDNA Extraction Kit (Biotechrabbit, Berlin, Germany) with additional mechanical lysis steps. First, 1 mL of swab suspension was transferred to a 2 mL lysis tube and centrifuged at 2000 revolutions per minute (rpm) for 5 min to pellet the bacterial cells. After removing the supernatant, the sample was added to 200 µL of lysis buffer from the extraction kit and lysed using a TissueLyser LT with a 5 mm stainless steel bead (Qiagen, Hilden, Germany) at 50 Hz for 5 min. The unlysed residue was pelleted again by centrifuging at 12 000 rpm for 2 min. The lysate was then processed following the standard protocol from the extraction kit.
Genup gdna extraction kit
Manufactured by Biotechrabbit
Sourced in Germany
The GenUP gDNA extraction kit is a laboratory tool designed for the extraction and purification of genomic DNA from a variety of sample types. The kit utilizes a spin column-based method to efficiently isolate high-quality genomic DNA, which can then be used for further downstream applications.
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Lab products found in correlation
4 protocols using genup gdna extraction kit
1
Efficient DNA Extraction from Bacterial Swabs
2
Microbiota Analysis of Mouse Fecal Samples
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Feces from nine mice (0.25 g per mouse) from different cages in each experimental group were divided into three samples per group (three mice per sample) before performing microbiota analysis. Total DNA from feces was extracted by GenUP gDNA extraction kit (Biotechrabbit, Germany) followed by 16S rDNA amplification for next-generation sequencing (NGS) with Illumina platform as previously published (42 (link)). For data analysis, the raw data were de-multiplexed by miSeq reporter software (version 2.6.2.3). Paired-end FASTQ sequences were then analyzed with QIIME2 pipeline (version 2018.8) (43 (link)). After that, joined reads were de-duplicated and clustered with 97% similarity by VSEARCH (44 (link)). Chimeric sequences were filtered out by UCHIME algorithm (45 (link)). The filtered reads were classified based on 99% operational taxonomic units (OTUs) clustered 16S Greengene database (2013.8) (46 (link)) using vsearch algorithm.
3
DNA Extraction from Tissue and Tape Strips
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Total DNA was extracted using the GenUP™ gDNA extraction kit (Biotechrabbit, Germany) with a modified protocol for the sample processing steps. For tissue (biopsy) samples, the tissue was homogenized with 400 µL LYSIS LG buffer in a 2 mL microtube with a tissue lyzer (50 Hz for 5 min). The suspension was then centrifuged for 5 min at 15,000 × g. The supernatant was treated with 25 µL proteinase K and 3 µL RNase A, and subsequently extracted according to the manufacturer’s guidelines. For the tape strip, the strip was cut into eight equal parts and directly incubated with 400 µL Buffer LYSIS LG, 25 µL Proteinase K, and 3 µL RNase A at 50 ℃ for 24 h. The supernatants were then collected and extracted according to the manufacturer’s standard protocol.
4
Roundworm Genomic DNA Extraction
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The individual large roundworm was homogenized by high-speed shaking at 50 Hz for 5 min in plastic tubes with lysis buffer and small stainless beads using TissueLyser LT (Qiagen, Germany). The genomic DNA was extracted from human feces and intestinal tissue of large roundworm samples using the GenUP gDNA extraction kit (Biotechrabbit, Germany) following the manufacturer’s recommendations after removal of small amounts of debris by centrifugation at 13,000 rpm for 5 min. The concentrations and quality of DNA were measured using a NanoPhotometer C40 (Implen, Germany).
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