Female and male NSG (NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ) mice from the Jackson Laboratories (Bar Harbor, ME, USA) or female Balb/c mice (BALB/cJRj, Janvier labs, Le Genest-Saint-Isle, France) were used for all experiments. Human CD34+ hematopoietic stem cells were isolated from human cord blood by magnetic separation (EasySep™ Human Cord Blood CD34 Positive Selection Kit II, STEMCELL technologies, Cologne, Germany) following the manufacturer’s protocol and purity controlled by flow cytometry. Humanized NSG (huNSG) mice were generated by engraftment of 100,000 hCD34+ cells (of a donor mix) at the age of 6 – 8 weeks, similar to the procedures described in earlier publications (15 (link)–18 (link)). Briefly, mice received whole-body irradiation with a sub-lethal dose of 2 Gy and hematopoietic stem cells were administered intravenously 2 hours later. For the generation of murinized NSG mice (muNSG), mice were treated as the huNSG mice, but received 100,000 bone marrow cells from a Balb/c donor instead of human CD34+ cells. The peripheral blood of huNSG mice was analyzed for the presence and frequency of murine CD45+, as well as human CD45+, CD3+ and CD20+ cells at week 18 post engraftment by flow cytometry.
Balb cjrj
Manufactured by Janvier Labs
Sourced in France
The BALB/cJRj is a mouse strain developed for use in laboratory research. It is a common inbred strain with a well-characterized genetic background. The BALB/cJRj strain is suitable for a variety of experimental applications.
Automatically generated - may contain errors
Lab products found in correlation
C57bl 6jrj, by Janvier Labs (4 mentions)
Nod cg prkdcscid il2rgtm1wjl szj mice, by Jackson ImmunoResearch (2 mentions)
129s2 svpasorlrj, by Janvier Labs (2 mentions)
C57bl 6 mice, by Janvier Labs (2 mentions)
C57bl 6j, by Janvier Labs (2 mentions)
Easysep human cord blood cd34 positive selection kit 2, by STEMCELL (1 mentions)
C57bl 6jrj mice, by Janvier Labs (1 mentions)
Balb cannrj foxn1nu nu, by Janvier Labs (1 mentions)
C3h henrj, by Janvier Labs (1 mentions)
Vac alu 250, by InvivoGen (1 mentions)
Fvb ncrl, by Charles River Laboratories (1 mentions)
129s2 svpascrl, by Charles River Laboratories (1 mentions)
129x1 svj, by Jackson ImmunoResearch (1 mentions)
17 protocols using balb cjrj
1
Generation of Humanized and Murinized NSG Mice
2
Mice Sourcing and Housing for Immunology Studies
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BALB/cJRj and C57BL/6JRj mice were purchased from commercial vendors (Janvier, Le Genest Saint Isle, France). B6.129P2-Igh-Jtm1Cgn/J (JHT) mice were bred in the animal facility of Helmholtz Center for Infection Research, Braunschweig. The animals were housed under SPF conditions at HZI or Hebrew University in Jerusalem and handled according to good animal practice as defined by the Federation of Laboratory Animal Science Associations (FELASA). The animal experiments were approved by the Lower Saxony State Office of Consumer Protection and Food Safety and the Hebrew University Medical School Ethics committee.
3
Interferon Signaling Pathway Knockout Mice
4
Murine Model for Biomedical Research
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Male mice weighting c.a. 22 g (BALB/cJRj, 9 weeks, Janvier; see below for number of animals) were used. The animals were maintained and handled in accordance with the Guidelines for Accommodation and Care of Animals (European Convention for the Protection of Vertebrate Animals Used for Experimental and Other Scientific Purposes) and internal guidelines. All experimental procedures were approved by the Ethical Committee of CIC biomaGUNE and by local authorities (Diputación Foral de Guipúzcoa) before conducting experimental work.
5
Mouse models in immunology research
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BALB/cJRj and C57Bl/6 mice were from Janvier. Transgenic C57Bl/6 FOXP3-GFP mice that express green fluorescent protein (GFP) in FOXP3+ cells were kindly provided by Dr. Malissen of the Centre d’immunologie de Marseille Luminy (France). CD3 KO mice were from CDTA of Orléans (France). Animals were maintained in our animal facility under specific pathogen-free conditions in agreement with current European legislation on animal care, housing, and scientific experimentation. All procedures were approved by the Regional Ethics Committee on Animal Experimentation No. 5 of the Ile-de-France region (Ce5/2012/031).
6
DNA Immunization in Murine Models
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All animal experiments performed during this study were approved by an external ethics committee authorized by the North Rhine-Westphalia Ministry for Environment and Nature Protection, Agriculture and Consumer Protection with the project licenses (AZ 84-02.04.2015.A082, approved at 28.07.2015). Six- to eight-week old BALB/cJRj and C57BL/6J mice were purchased from Janvier Laboratories (Le Genest-Saint-Isle, France) while Myd88/Trif−/− mice were provided by C. Kirschning [34 (link)]. Card9−/− mice were generated by Jürgen Ruland and backcrossed at the TiHo Hannover [35 (link)]. All mice were housed in individually ventilated cages in accordance to the national law and institutional guidelines at the animal facility of the Faculty of Medicine, Ruhr-University Bochum. DNA immunizations were performed as described previously [13 (link)]. Briefly, animals were anesthetized with ketamine/xylazine and received 15 µg of plasmid DNA in 30 µL of PBS in each hind leg followed by local application of an electric pulse (TriGrid; Ichor Medical, San Diego, CA, USA). A boost immunization following the same schedule was performed four weeks after the priming.
7
Mouse Models for Immunological Research
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BALB/cJRj and BALB/cAnNRj-Foxn1nu/nu were purchased from Janvier labs (Roubaix, FR). NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ mice were originally obtained from the Jackson Laboratory and provided by Christian Münz (University of Zurich, Switzerland). Mice were kept under specific pathogen-free conditions in individually ventilated cages at the Laboratory Animal Services Center at the University of Zurich. Mice had access to food and water ad libitum and were maintained on a 12-h light/dark cycle with environmental enrichment. All experiments were performed with 8–14-weeks-old female mice in accordance with the Swiss federal and cantonal regulations on animal protection and were approved by The Cantonal Veterinary Office Zurich (156/2018).
8
Validating Pathogen-Free Ticks for Mice Studies
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Six- to 8-week-old specific-pathogen-free (SPF) female C57BL/6JRj, BALB/cJRj, and C3H/HeNRj mice (Janvier, France) were used for the experiments. The trichostrongyloid nematode H. polygyrus was maintained by serial passage in C57BL/6 mice. Specific-pathogen-free larval I. ricinus ticks were obtained from batches of eggs from two adult females after they had fed on laboratory beagle dogs (license number H0078/10, Landesamt für Gesundheit und Soziales, Berlin, Germany). The absence of tick-borne pathogens in the tick larvae used for experimentation was surveyed by published PCRs (33 (link), 34 (link)) for the detection of a 153-bp fragment of the hbb gene of Borrelia burgdorferi sensu lato , a 203-bp fragment of the gltA gene of spotted fever Rickettsia spp., a 602- to 639-bp fragment of the 18S rRNA gene of piroplasmida (33 (link)), and a 257-bp fragment of the 16S rRNA gene of the Anaplasmataceae (34 (link)) in DNA isolated (33 (link)) from subsets of 20 larvae randomly chosen from each tick batch. Borrelia afzelii -infected I. ricinus nymphs were derived from larvae that had engorged on experimentally infected mice (license number 23-2347-A-24-1-2010, Landesamt für Umwelt, Gesundheit, und Verbraucherschutz, Potsdam, Germany).
9
Murine Models for Immunological Studies
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6- to 8-week-old BALB/c JrJ and C57BL/6 JrJ female mice were acquired from Janvier Labs (France). The mice were acclimated for one week before the initiation of the experiments. The experiments were performed in accordance with relevant guidelines and regulations, conducted under license 2017-15-0201-01209 from the Danish Animal Experimentation Inspectorate in accordance with the Danish Animal Experimentation Act (BEK nr. 12 of 7/01/2016), which is compliant with the European directive (2010/63/EU).
10
BALB/c Mice Husbandry and Maintenance
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Female 6 -week-old BALB/c mice (BALB/cJRj) were purchased from Janvier and distributed randomly. All the mice were maintained in a pathogen-free animal facility under standard 12 h light/12 h dark cycle at 21 C with access to normal chow and water ad libitum.
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