All primary antibodies were targeted against human antigens. The antibodies used for analysis included CD16 (clone 3G8, PE-Cy7), CD107a (clone H4A3, fluorescein isothiocyanate [FITC]), IFN-γ (clone B27, APC), CD44 (clone 515, PE, all BD Biosciences), CD56 (clone HCD56, Brilliant Violet 510), CD62-L (clone DREG-56, PerCP/Cy5.5), CXCR4 (clone 12G5, PE), CXCR3 (clone G025H7, APC), neutralizing IL-6 (clone MQ2-13A5), neutralizing IFN-α (clone MMHA6, all Tebu-Bio, Le Perray-en-Yvelines, France), neutralizing IFN-β (PeproTech, Hamburg, Germany), Perforin (clone dG9, FITC, Biolegend, Fell, Germany), TIM-3 (clone F38-2E2, PerCP-eFluor710, eBioscience, Frankfurt, Germany), Nkp30 (clone Z25, RPE), Nkp40 (clone Z231, RPE), Nkp46 (clone BAB281, RPE), NKG2A (clone Z199), CD158b (all Beckman Coulter, Krefeld, Germany), NKG2D (clone 149810, RPE), neutralizing TGF-β1,2,3 (clone 1D11, both R&D Systems, Wiesbaden, Germany), CDKN2A/p16INK4a (rabbit IgG, Abcam, Cambridge, UK) and phospho-mTOR (rabbit IgG, Cell Signaling, Frankfurt, Germany). As secondary coupled antibodies, goat anti-rabbit Alex488 (Dianova) was used.
Cd44 clone 515
Manufactured by BD
Sourced in France
CD44 (clone 515) is a lab equipment product used for the detection and analysis of the CD44 cell surface receptor. CD44 is a transmembrane glycoprotein involved in cell-cell interactions, cell adhesion, and migration. This product can be used in various research applications, including flow cytometry, immunohistochemistry, and other immunoassays.
Automatically generated - may contain errors
2 protocols using cd44 clone 515
1
Comprehensive Immune Cell Phenotyping
2
Immunophenotyping of Mesenchymal Stem Cells
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As mentioned by Darzi S, et al30 (link), aliquots of 105 cells/100 μl were incubated separately with phycoerythrin (PE)-conjugated mouse anti human CD29 (clone 04-MAR; BD Pharmingen), CD73 (clone AD2; BD Pharmingen), CD44 (clone 515; BD Pharmingen), CD133 (clone TMP4; eBioscience), and CD105 (clone 43A3; BioLegend) for 40 min at 4°C.
To assess Octamer-binding transcription factor 4 (OCT-4) expression, the cells were washed with 0.1% saponin-permeabilized cells and treated with primary rabbit antihuman OCT-4 antibody (Abcam) for 40 min and then incubated with FITC-conjugated goat anti-rabbit Ig (Sigma-Aldrich) for 30 min. Isotype IgG was used as negative controls (clone MOPC-21; BD Pharmingen). Afterwards, all cell suspensions were washed twice with PBS-FBS, fixed in 1% formaldehyde solution, and analyzed using a flow cytometer (Partec GmbH, Munster, Germany) and appropriate isotype controls.
To assess Octamer-binding transcription factor 4 (OCT-4) expression, the cells were washed with 0.1% saponin-permeabilized cells and treated with primary rabbit antihuman OCT-4 antibody (Abcam) for 40 min and then incubated with FITC-conjugated goat anti-rabbit Ig (Sigma-Aldrich) for 30 min. Isotype IgG was used as negative controls (clone MOPC-21; BD Pharmingen). Afterwards, all cell suspensions were washed twice with PBS-FBS, fixed in 1% formaldehyde solution, and analyzed using a flow cytometer (Partec GmbH, Munster, Germany) and appropriate isotype controls.
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