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Igg isotype control antibody

Manufactured by Abcam
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The IgG isotype control antibody is a laboratory reagent used to establish appropriate background staining levels in immunoassays. It is an antibody of the same isotype as the primary antibody under investigation, but with specificity for an antigen not present in the sample being analyzed.

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IgG isotype control Isotype control antibody Control IgG antibody Isotype control Isotype IgG Control IgG

2 protocols using igg isotype control antibody

1

Intranasal anti-IFNAR1 antibody effects

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Day 3 post-infected mice were anesthetized and then injected intranasally with either 50 μg of IgG isotype control antibody (Abcam) or 50 μg of anti-IFNAR1 antibody (eBioscience). After 3 days, infiltrating cells were counted and then stained with specific Abs against with Gr1, CD11b, Ly6G, Ly6C and CCR2.
Lin S.J., Lo M., Kuo R.L., Shih S.R., Ojcius D.M., Lu J., Lee C.K., Chen H.C., Lin M.Y., Leu C.M., Lin C.N, & Tsai C.H. (2014). The pathological effects of CCR2+ inflammatory monocytes are amplified by an IFNAR1-triggered chemokine feedback loop in highly pathogenic influenza infection. Journal of Biomedical Science, 21(1), 99.
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2

Harmine Enhances Angiogenic Potential of RANKL-Treated Cells

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To test whether harmine could augment the pre-angiogenic ability of RANKL-stimulated RAW264.7 cells by promoting PDGF-BB production, HMECs were cultured in MCDB131 medium under different treatment conditions: 1) CMVehicle + IgG group (treated with CM from un-induced RAW264.7 cells and isotype IgG); 2) CMRANKL + IgG group (treated with CM from RANKL-treated RAW264.7 cells and isotype IgG); 3) CMRANKL + Harmine + IgG group (treated with CM from RANKL + harmine-treated RAW264.7 cells and isotype IgG); 4) CMRANKL + Harmine + PDGF-BB Ab group (treated with CM from RANKL + harmine-treated RAW264.7 cells and PDGF-BB-neutralizing antibody). To test the direct effect of harmine on the angiogenic activities of endothelial cells, HMECs were treated with 3 μg/mL harmine or vehicle (DMSO). The IgG isotype control antibody was obtained from Abcam (Cambridge, Cambs, Britain). PDGF-BB-neutralizing antibody was purchased from R&D system (Minneapolis, MN, USA).
Huang J., Yin H., Rao S.S., Xie P.L., Cao X., Rao T., Liu S.Y., Wang Z.X., Cao J., Hu Y., Zhang Y., Luo J., Tan Y.J., Liu Z.Z., Wu B., Hu X.K., Chen T.H., Chen C.Y, & Xie H. (2018). Harmine enhances type H vessel formation and prevents bone loss in ovariectomized mice. Theranostics, 8(9), 2435-2446.
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