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D4 cortisol

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D4-cortisol is a deuterated form of the steroid hormone cortisol. It is used as an internal standard in analytical methods for the quantification of cortisol in biological samples.

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Lab products found in correlation

Cortisone, by Merck Group (2 mentions) Estrone, by Merck Group (2 mentions) Cortisol, by Merck Group (2 mentions) Progesterone, by Merck Group (2 mentions) Dihydrotestosterone dht, by Merck Group (2 mentions) Dehydroepiandrosterone dhea, by Merck Group (2 mentions) 17β0 estradiol, by Merck Group (2 mentions) D5 estradiol, by CDN Isotopes (2 mentions) D5 testosterone, by CDN Isotopes (2 mentions) D4 dht, by CDN Isotopes (2 mentions) D9 progesterone, by CDN Isotopes (2 mentions) Hplc grade methanol, by Merck Group (2 mentions) Formic acid, by Merck Group (2 mentions) Testosterone, by Merck Group (2 mentions) 2 propanol, by Merck Group (2 mentions) 7 oxo dhea, by Steraloids (2 mentions) D3 dhea, by Steraloids (2 mentions) 2 hydrazinopyridine, by Merck Group (2 mentions) Ammonium formate, by Merck Group (2 mentions) Trifluoroacetic acid, by Merck Group (2 mentions)

4 protocols using d4 cortisol

1

Steroid Reference Materials for LC-MS/MS

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Steroid reference materials were obtained from Sigma-Aldrich (St. Louis, MO): estrone, 17β0-estradiol, testosterone, dihydrotestosterone (DHT), dehydroepiandrosterone (DHEA), progesterone, cortisol and cortisone. The deuterated internal standards, d5-estradiol, d5-testosterone, d4-DHT, d9-progesterone and d4-cortisol were obtained from CDN Isotopes (Point-Claire, Quebec, Canada). HPLC grade methanol, 2-propanol, water, and formic acid were purchased from Sigma.
Kapoor A., Lubach G., Hedman C., Ziegler T.E, & Coe C.L. (2014). Hormones in Infant Hair at Birth Provide a Window into the Fetal Environment. Pediatric research, 75(4), 476-481.
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2

Steroid Reference Materials for LC-MS/MS

Check if the same lab product or an alternative is used in the 5 most similar protocols
Steroid reference materials were obtained from Sigma-Aldrich (St. Louis, MO): estrone, 17β0-estradiol, testosterone, dihydrotestosterone (DHT), dehydroepiandrosterone (DHEA), progesterone, cortisol and cortisone. The deuterated internal standards, d5-estradiol, d5-testosterone, d4-DHT, d9-progesterone and d4-cortisol were obtained from CDN Isotopes (Point-Claire, Quebec, Canada). HPLC grade methanol, 2-propanol, water, and formic acid were purchased from Sigma.
Kapoor A., Lubach G., Hedman C., Ziegler T.E, & Coe C.L. (2014). Hormones in Infant Hair at Birth Provide a Window into the Fetal Environment. Pediatric research, 75(4), 476-481.
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3

Steroid Extraction and Quantification

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Cortisol, cortisone and dehydroepiandrosterone (DHEA) were purchased form Koch-Light Laboratories LTD (Colnbrook, Great Britain), 7α-OH-DHEA, 7β-OH-DHEA, 7-oxo-DHEA, 16α-OH-DHEA and D3-DHEA were from Steraloids (Newport, USA). D4-Cortisol was from CDN isotopes (Ponte-Claire, Canada).
2-hydrazinopyridine, ammonium formate, methyl tertbutyl ether and trifluoroacetic acid were from Sigma-Aldrich (St. Louis, USA). LC-MS grade methanol, water and diethyl ether were from Merck AG (Darmstadt, Germany). The physiological solution (0.9 % sodium chloride) was from B-Braun (Melsungen AG, Germany). [1,2,6,7-3H]Cortisol, specific radioactivity 3.04 TBq/mmol was from Amesrham Biosciences, Inc.
(Amersham, UK).
Máčová L., Sosvorová L., Vítků J., Bičíková M., Hill M., Zamrazilová H., Sedláčková B, & Stárka L. (2015). Steroid hormones related to 11beta-hydroxysteroid dehydrogenase type 1 in treated obesity. Physiological research, 64(Suppl 2).
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4

Steroid Quantification Methodology

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Cortisol, cortisone and dehydroepiandrosterone (DHEA) were purchased from Koch-Light Laboratories LTD (Colnbrook, Great Britain); 7α-hydroxy-DHEA, 7β-hydroxy-DHEA, 7-oxo-DHEA, 16α-hydroxy-DHEA and D3-DHEA were from Steraloids (Newport, USA). D4-Cortisol was from CDN isotopes (Ponte-Claire, Canada). 2-hydrazinopyridine, ammonium formate, methyl tert-butyl ether, trifluoroacetic acid, homocystine, dithiothreitol, pyridine, p-fluoro-DL-phenylalanine, ethyl chloroformate, isooctane, ethanol, chloroform, sodium chloride, hydrochloric acid and potassium oxalate monohydrate were from Sigma-Aldrich (St. Louis, USA). LC-MS grade methanol and water as well as diethyl ether were from Merck AG (Darmstadt, Germany). The physiological solution was from B-Braun (Melsungen AG, Germany).
Sosvorova L., Mohapl M., Hill M., Starka L., Bicikova M., Vitku J., Kanceva R., Bestak J, & Hampl R. (2015). Steroid hormones and homocysteine in the outcome of patients with normal pressure hydrocephalus. Physiological research, 64(Suppl 2).
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