Supernatant was collected from sorted CD4+ and CD8+ T-cells cultured in the presence of anti-CD3 and anti-CD28 (5 and 2 μg/ml, respectively) coated 24-well plate for 24 hours for a CBA using a ProcartaPlex Mouse Cytokine/Chemokine Panel 1A 36-Plex kit (EPX360-26012-901; Invitrogen). Data was acquired on a Luminex 200 (R&D Systems).
Procartaplex mouse cytokine chemokine panel 1a 36 plex kit
Manufactured by Thermo Fisher Scientific
The ProcartaPlex Mouse Cytokine/Chemokine Panel 1A 36-Plex kit is a multiplex assay that simultaneously measures the levels of 36 different mouse cytokines and chemokines in a single sample. The kit utilizes bead-based technology to detect and quantify the target analytes.
Automatically generated - may contain errors
Lab products found in correlation
Luminex 200, by R&D Systems (3 mentions)
Lysozyme, by Merck Group (1 mentions)
Calf thymus dna, by Merck Group (1 mentions)
Genomic dna buffer set, by Qiagen (1 mentions)
Proteinase k, by Merck Group (1 mentions)
Rnase a, by Qiagen (1 mentions)
Methylated bsa mbsa, by Merck Group (1 mentions)
Deoxyribonucleic acid, by Merck Group (1 mentions)
Incomplete freund s adjuvant, by Merck Group (1 mentions)
4 protocols using procartaplex mouse cytokine chemokine panel 1a 36 plex kit
1
Multiplex Cytokine Profiling of Activated T-Cells
2
Cytokine Profiling of Activated T-cells
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Supernatant was collected from sorted CD4+ and CD8+ T-cells cultured in the presence of aCD3/aCD28 (2 and 5 μg/ml, respectively) coated 24-well plate for 24 hours for a CBA using a ProcartaPlex Mouse Cytokine/Chemokine Panel 1A 36-Plex kit (EPX360-26012-901; Invitrogen). Data was acquired on a Luminex 200 (R&D Systems).
3
Cytokine Profile of Activated T Cells
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PBL was incubated in a 96-well round bottom plate coated with agonistic aCD3/aCD28 (100 µL solution per well of aCD3 (5 µg/mL, 145-2 C11, BD Biosciences) and aCD28 (2 µg/mL, 37.5.1, BD Biosciences)) prior to staining. Plates were incubated for 44 hours in cell culture conditions (37°C, 5% CO2, 95% humidity) for a cytokine bead array using a ProcartaPlex Mouse Cytokine/Chemokine Panel 1A 36-Plex kit (EPX360-26012-901; Invitrogen). Data were acquired on a Luminex 200 (R&D Systems).
4
Escherichia coli DNA Extraction and Analysis
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Deoxyribonucleic acid, sodium salt, from Escherichia coli strain B; calf thymus DNA; incomplete Freund's adjuvant (IFA); proteinase K; lysozyme; and methylated BSA (mBSA) were purchased from Sigma-Aldrich (St Louis, MO). Novagen pig genomic DNA was purchased from MilliporeSigma (St Louis, MO). Genomic Maxi-tips, Genomic DNA Buffer sets, and RNase A were purchased from Qiagen (Germantown, MD). TE buffer, pH 8.0, was purchased from Amresco Life Science/VWR (Radnor, PA). A 200-bp DNA ladder (New England Biolabs), 96-well Maxisorp microplates, and T-PER (Tissue Protein Extraction Reagent) were purchased from New Thermo Scientific (Waltham, MA). Goat anti-mouse IgG (H + L) conjugated to horseradish peroxidase was purchased from Novus Biologicals (Littleton, CO). TMB substrate (BD OptEIA Reagent) was purchased from BD Biosciences (San Jose, CA). A ProcartaPlex Mouse Cytokine/Chemokine Panel 1A 36plex Kit was purchased from Invitrogen (Carlsbad, CA).
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